Preparation and cryopreservation method and application of human placental subchorionic large blood vessel tissue
A cryopreservation method and chorionic membrane technology, applied in the preservation, application, animal husbandry and other directions of human or animal body, can solve the problems of the influence of cell activity, loss of the function of cryopreserved tissue to protect cells, etc., and achieve the effect of improving activity
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Embodiment 1
[0038] The cryopreservation method of human placental subchorionic large blood vessel tissue of embodiment 1
[0039] Placenta collection: Select a healthy placenta without infectious diseases and obstetric complications, obtain the consent of the parturient and sign the informed consent; for normal collection, the collected placenta will be transported to the laboratory within 48 hours, and various necessary tests will be carried out, such as Detection of infectious diseases such as viruses, detection of bacterial contamination, etc.
[0040] The method of cryopreservation, the steps are as follows:
[0041] (1) Wash the placenta (to remove dirt and microbial contamination); cut off the decidua along the edge of the placenta, and remove the amniotic membrane.
[0042] (2) Separate the above-mentioned placental fetal surface chorion and large blood vessels from which the amniotic membrane has been removed (keep the subchorionic large blood vessels intact as much as possible),...
Embodiment 2
[0050] Recovery after embodiment 2 cryopreservation
[0051] According to the method of Example 1, after cryopreservation for 6 months, recovery was carried out, and mesenchymal stem cells were induced and isolated. The steps were as follows:
[0052] Take the cryopreserved placental subchorionic large blood vessel tissue out of the liquid nitrogen, place it in the gas phase for 10 minutes to equilibrate, and then quickly place the cryopreservation bag in a water bath at 37°C to 42°C; quickly transfer the cryopreservation bag to a safety cabinet after dissolving , open the cryopreservation bag, gently remove the placental subchorionic large blood vessel tissue with tweezers, put it into the resuscitation solution of three times the reference concentration at 4°C (pre-cooled to 4°C in advance), equilibrate for 1 minute, take it out, and then put it in 4°C ℃ double standard concentration resuscitation solution (pre-cooled to 4 ℃ in advance), equilibrate for 1 to 3 minutes, take ...
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