Human placenta, umbilical cord mesenchyma stemcell stock and its construction method

Abstract

The invention discloses a human placenta, umbilical cord mesenchyme stem cell bank and its making method. The method includes the following steps: the first is taking human placenta, umbilical cord to test; smashing after cleaning by phosphate buffer; adding phosphate buffer to dilute; the second is adding collagenase to digest; the third is adding phosphate buffer; the fourth is adding trypsinization; the fifth is mixing the cell from the second and fourth, centrifuging, removing supernatant, cleaning by the phosphate buffer, centrifuging, and removing supernatant; the mesenchyme stem cell are gained; the sixth is freezing them in liquid nitrogen; saving by ABO / Rh subtype and HLA subtype; setting recallable cell information file. The invention is newborn storage placenta and umbilical cord mesenchyme stem cell. It can offer mesenchyme stem cell to treat the disease for personal, family and others.

Description

technical field

[0001] The invention relates to a stem cell bank and a construction method thereof, in particular to a human placenta and umbilical cord mesenchymal stem cell bank and a construction method thereof. technical background:

[0002] Mesenchymal stem cells (MSCs) are a type of stem cells with two important characteristics of stem cells: strong self-proliferation ability and multi-differentiation potential. MSCs originated from mesoderm, and theoretically, it can differentiate to other mesoderm tissues. Recent studies have shown that under suitable in vivo or in vitro conditions, MSCs can not only differentiate into mesoderm mesenchymal tissue, but also maintain the differentiation potential of endoderm, and can differentiate into nerve cells, epithelial cells, cardiomyocytes, and osteoblasts. MSCs are co-cultured with fetal mouse midbrain or striatal cells, which can differentiate into neurons and glial cells, and MSCs are transplanted around the myocardial isch...

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