Umbilical cord preserving fluid and preparation method thereof

A technology of preservation solution and umbilical cord, which is applied in the field of umbilical cord preservation solution, can solve the problems that the basal medium is not up to the clinical use level, the activity of stem cells is affected, and the storage time is short, so as to achieve low pollution rate, reduce accumulation, and reduce time The effect of restriction
CN102334472AActive Publication Date: 2012-02-01江苏省北科生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
江苏省北科生物科技有限公司
Publication Date
2012-02-01

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Abstract

The invention discloses an umbilical cord preserving fluid for temporary preservation of umbilical cord after collection before separation. The umbilical cord preserving fluid comprises the following main components: sodium chloride, glucose, human serum albumin, potassium chloride and anhydrous calcium chloride, as well as auxiliary components: cefoperazone sodium and phenol red, wherein the main components have the effects on maintaining osmotic equilibrium of inner cells of the umbilical cord tissue, keeping the umbilical cord in a wet state and providing nutrient content for a short period of time; and the auxiliary components have the effects on preventing probable pollution and prompting happened pollution. The preserving fluid can be used for preserving the activity of mesenchyme stem cells in the umbilical cord, the umbilical cord can be effectively preserved at 2-10 DEG C for at least 72 hours, the activity of the separated mesenchyme stem cells is minimally influenced by time, the time limit from collection to preparation of the umbilical cord is greatly reduced, and all the used components meet a clinical use standard and are capable of effectively reducing the pollution probability.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a preservation solution for short-term preservation of umbilical cords from collection to separation. Background technique

[0002] The umbilical cord is a tubular structure that connects the fetus and the placenta. In addition to two arteries and one vein and three blood vessels, the loose jelly-like substance (we call it Wharton's jelly) between the blood vessels and the amniotic membrane also contains rich umbilical cord mesenchyme. stem cells. Mesenchymal stem cells are an important member of the stem cell family. They have the characteristics of multi-directional differentiation potential, hematopoietic support and promotion of stem cell implantation, immune regulation and self-replication. They are ideal seed cells for repairing tissue and organ damage caused by aging and disease. Umbilical cord mesenchymal stem cells are getting more and more attention because of thei...

Claims

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