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Use of icariin in inducting dry cell body in-vitro directional differentiation

A technology of icariin, stem cells, applied in the field of cell biology and pharmacology

Inactive Publication Date: 2006-11-29
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] But so far, there is no research report on the effect of icariin on the directed differentiation of stem cells into nerve cells at home and abroad

Method used

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  • Use of icariin in inducting dry cell body in-vitro directional differentiation
  • Use of icariin in inducting dry cell body in-vitro directional differentiation
  • Use of icariin in inducting dry cell body in-vitro directional differentiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Icaritin-induced differentiation of embryonic stem cells (ES cells) into neural cells in vitro

[0022] 1. ES cell support culture:

[0023] ES cell D3 cell line (ES-D3 cell) in the basal medium (containing high glucose DMEM, non-essential amino acids, 10% fetal bovine serum, leukemia inhibitory factor), press 1 × 10 6 Cells / ml were inoculated on feeder cells (mouse fibroblasts), and subcultured for 2 to 3 days.

[0024] 2. Culture of icariin-induced ES cells to differentiate into neurons:

[0025] ES-D3 cells were digested with trypsin, and made into a single-cell suspension with differentiation medium (containing high-sugar DMEM, non-essential amino acids, 10% fetal bovine serum, and no leukemia inhibitory factor), at about 600 cells / 30 μL Inoculate on the inner surface of the lid of the petri dish, turn the lid over to form a hanging drop, and culture it in an incubator for 3 days. Thereafter, the hanging drops that had formed embryoid bodies were transferred to a...

Embodiment 2

[0026] Example 2 Use of icariin to induce directed differentiation of ES cells into nerve cells in drug efficacy evaluation If the embryoid body obtained in Example 1 can appear nerve-like cells (the length of the cell process is more than 5 times the diameter of the cell body) ), then use it as an indicator of directed differentiation into nerve cells, calculate the ratio of embryoid bodies that differentiated into neural-like cells to the total number of embryoid bodies, and make the dose-effect and sum of the drug icariin's induced differentiation of ES-D3 cells Aging curve (see figure 1 ), which is used as the evaluation index of drug efficacy. It can be used for drug development and screening of active ingredients of traditional Chinese medicine. see figure 1 , the effect of icariin on inducing ES cells to differentiate into nerve cells is very obvious, and has a good dose-effect relationship. When it is co-incubated with ES cell embryoid bodies for 8-10 days, more than...

Embodiment 3

[0028] Example 3: The use of icariin in inducing ES cells to differentiate into nerve cells in other aspects

[0029] Using icariin to induce directed differentiation of ES cells into nerve cells, if it can be successfully introduced into the damaged nervous system and work together with host cells, this pathway can become a good way for transplantation to treat neurodegenerative diseases and repair and rebuild damaged nerve tissue. An important source of tissue cells, a large number of nerve cell clones can be cultivated by this method, providing tissue engineering and other research.

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Abstract

The invention provides icariin application in inducing stem cell in vitro orientation differentiates to several single type cells. The stem cell said includes embryonic stem cell, nerve stem cell and marrow mesenchyme stem cell. The single type cell includes nerve cell, bone cell, islet cells and endothelial cell. This invention also relates to application of the single type cell in preparing medicine of stem cell transplant curing nerve degenerative diseases, and its application in preparing cell differentiation agent that used to repair recovery injured nerve tissue, and its application in high efficiency drug effect screening model rebuilding and initial screening and estimating by using the model. The new applications of the icariin is extended in this invention, the fact of icariin contains pharmacy activity clarified, so it provides material basis for Chinese traditional medicine prevention and cure effect. The clarifying of drug effect mechanism provides reference for new medicine Chinese metical modern development with self-owned intellectual property right.

Description

technical field [0001] The invention belongs to the field of cell biology and pharmacology, and relates to the use of a traditional Chinese medicine monomer compound, in particular to the use of icariin in inducing the directional differentiation of stem cells in vitro, which can be used for stem cell transplantation to treat neurodegenerative diseases and repair and rebuild damage Stimulator of cell differentiation in nervous tissue. Background technique [0002] Herba Epimedii (Herba Epimedii) is a plant of the genus Epimedium in the family Berberidaceae, which has the functions of tonifying kidney and strengthening yang, expelling wind and dampness. Modern pharmacological research has proved that Epimedium contains special chemical components and significant biological activities, and is a traditional Chinese medicinal material with a wide range of pharmacological activities. Flavonols in Epimedium have pharmacological activities such as treating cardiovascular and cereb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06C12N5/08A61K35/12A61P25/00A61K35/30A61K35/32A61K35/39A61K35/44C12N5/0735C12N5/0775C12N5/0797
Inventor 朱丹雁王志强楼宜嘉
Owner ZHEJIANG UNIV
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