Process for separation and culture of marrow mesenchyme stem cell

Abstract

The invention discloses a process for separation and culture of marrow mesenchyme stem cell, compared with the conventional methods, the invention realizes easy operation, shortened time of cell culture, high purity of mesenchyme stem cells obtained through separation, and rapid propagation. The obtained cells have homogeneous shapes of elongated shuttles. After passaging, the wall adhesion capacity of the cells is strong in 8 generation.

Description

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AI Technical Summary

Benefits of technology

This patented technology allows for easy separation and cultivative expansion of human blood platelets (HSC). It uses specialized media called BMMSCs instead of normal HSC, which means they cannot be grown into different types of cells like other adult organs. By doing it under specific environmental factors such as temperature, pH levels, nutrients, etc., these cells become more matured over longer periods compared to traditional methods. Additionally, there're no risk involved during their isolate process due to lack of receptors on them. Overall, this technique simplifies the procedure and makes it easier to study how pluripotential properties work together within an organism.

Problems solved by technology

This patented describes three ways how we could collect specific type of stem/progenitor cells called MSC' s which were discovered during embryonic development. These include Adhesion Screening Method, Dense Gradient Centrifuge, Flow Cytometer, Immunomechannelectromagnetism Beads, etc., each having its own advantages and disadvantages over existing techniques like those mentioned earlier. However, these new technologies may still require further improvements due to factors including lack of suitable source material and difficulty obtaining consistently pure samples containing many rare populations of MCSs.

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