Process for separation and culture of marrow mesenchyme stem cell

A technology of bone marrow mesenchyme and culture method, applied in the biological field, can solve the problems of low cell viability and large cell damage, and achieve the effect of good proliferation, fast growth speed and good growth condition

Inactive Publication Date: 2005-11-23
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for easy separation and cultivative expansion of human blood platelets (HSC). It uses specialized media called BMMSCs instead of normal HSC, which means they cannot be grown into different types of cells like other adult organs. By doing it under specific environmental factors such as temperature, pH levels, nutrients, etc., these cells become more matured over longer periods compared to traditional methods. Additionally, there're no risk involved during their isolate process due to lack of receptors on them. Overall, this technique simplifies the procedure and makes it easier to study how pluripotential properties work together within an organism.

Problems solved by technology

This patented describes three ways how we could collect specific type of stem/progenitor cells called MSC' s which were discovered during embryonic development. These include Adhesion Screening Method, Dense Gradient Centrifuge, Flow Cytometer, Immunomechannelectromagnetism Beads, etc., each having its own advantages and disadvantages over existing techniques like those mentioned earlier. However, these new technologies may still require further improvements due to factors including lack of suitable source material and difficulty obtaining consistently pure samples containing many rare populations of MCSs.

Method used

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  • Process for separation and culture of marrow mesenchyme stem cell
  • Process for separation and culture of marrow mesenchyme stem cell

Examples

Experimental program
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Effect test

Embodiment 1

[0037] (1) Primary culture:

[0038] Femur tissue from four-month-old fetuses who died after induction of labor by the water bag method was collected, cells in the bone marrow cavity were washed with D-Hank's solution, and centrifuged at 1200 rpm for 8 minutes after collection; the supernatant was discarded after centrifugation, and the cell pellet was blown with mixed culture medium I to make Form a cell suspension and perform cell counting; take the mixed culture solution I containing cells, and press the cell culture density of 1×10 6 Inoculate each cell / bottle into a 100ml cell culture bottle, make up to 8-10ml of the total culture medium with mixed culture solution I, culture for two days according to routine, discard half of the original medium, and then add 5ml of fresh mixed culture Solution I was placed in a carbon dioxide incubator to continue culturing.

[0039] (2) Subculture:

[0040] After the cells entered the logarithmic growth phase, remove the cell culture ...

Embodiment 2

[0048] (1) Primary culture:

[0049] Femur tissue from four-month-old fetuses who died after induction of labor by the water bag method was collected, cells in the bone marrow cavity were washed with D-Hank's solution, and centrifuged at 1200 rpm for 7 minutes after collection; the supernatant was discarded after centrifugation, and the cell pellet was blown with mixed culture medium I to make Form a cell suspension and perform cell counting; take the mixed culture solution I containing cells, and press the cell culture density of 1.5×10 6 1 cell / bottle, inoculated in a 100ml cell culture bottle, supplemented with mixed culture solution I until the total amount of culture solution was 8-10ml, cultured for two days as usual, discarded half of the original medium, and then added 4ml of fresh mixed Culture solution I was placed in a carbon dioxide incubator to continue culturing.

[0050] (2) Subculture:

[0051] After the cells entered the logarithmic growth phase, remove the ...

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Abstract

The invention discloses a process for separation and culture of marrow mesenchyme stem cell, compared with the conventional methods, the invention realizes easy operation, shortened time of cell culture, high purity of mesenchyme stem cells obtained through separation, and rapid propagation. The obtained cells have homogeneous shapes of elongated shuttles. After passaging, the wall adhesion capacity of the cells is strong in 8 generation.

Description

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Claims

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Application Information

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Owner NANKAI UNIV
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