Multipotential stem cell-derived mesoderm pedigree mesenchymal stem cell and preparation method thereof

A technology of pluripotent stem cells and mesenchymal stem cells, applied in the field of mesenchymal stem cells of mesoderm lineage and its preparation, can solve the problems of poor treatment effect, cell heterogeneity, uncontrolled differentiation path of cells, etc., and achieve strong proliferation and immune regulation capacity, resolution of source constraints, enhanced cytological or therapeutic effect
CN107937338AActive Publication Date: 2018-04-20SUN YAT SEN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SUN YAT SEN UNIV
Publication Date
2018-04-20

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Abstract

The invention discloses a multipotential stem cell-derived mesoderm pedigree mesenchymal stem cell and an induced differentiation method thereof. The multipotential stem cell-derived mesoderm pedigreemesenchymal stem cell is prepared by the following steps: performing in-vitro adherent culture on multipotential stem cells and maintaining the undifferentiation state of the multipotential stem cells; preparing unicellular or cell mass suspension from the cells, inoculating to a culture dish coated with matrix glue, and performing cultivation by using the multipotential stem cell culture liquid;after the cells adhere to the wall, adding GSK-3 pathway inhibitor combination into the culture liquid; after growing for 2 to 10 days, obtaining a mesoderm progenitor population; and after subculturing continuously for 2 to 6 times by using mesenchymal stem cell culture liquid, detecting the cell phenotype of mesenchyme. The defects that the human-derived mesenchymal stem cells and the mesenchymal stem cells derived from other non-finite induced way multipotential stem cells have heterogeneity and hybridity are overcome, and the obtained mesoderm pedigree mesenchymal stem cells have higher proliferation capability and immunoregulation capability; and the standardized induced differentiation process can guarantee that the cell populations obtained from different batches have high consistency.
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Description

technical field

[0001] The invention belongs to the field of biology and medicine, and more specifically, the invention relates to a mesoderm lineage mesenchymal stem cell derived from pluripotent stem cells and a preparation method thereof, including using special culture conditions and selection techniques. The present invention also relates to a therapeutic method using this population of mesenchymal stem cells of mesoderm lineage or its clinical application. Background technique

[0002] Mesenchymal stem cells (MSCs) are a kind of adult stem cells. They are a large class of pluripotent cells that exist in many tissues in the adult body, such as bone marrow, umbilical cord blood, fat, and peripheral blood. They are in a quiescent state under normal conditions. When the tissue or organ where they are located is damaged, they can quickly enter a proliferative state and maintain a great proliferative potential, and differentiate into different cell types to repair the damage...

Claims

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