Method for treating osteoarthritis by stem cells and composition

A technology for cell therapy and osteoarthritis, applied in the field of pharmacy, can solve the problems of cell number and expansion, decreased differentiation ability, unsuitable for batch preparation, etc., and achieves the effect of simple and mild preparation method and improved survival rate.

Active Publication Date: 2016-07-27
BOYALIFE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bone marrow-derived mesenchymal stem cells are not suitable for batch production due to the hidden danger of virus contamination, and as the age of the donor increases, the number of cells and the ability to expand and differentiate will decline significantly.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] Embodiment 1, the composition of placenta MSC

[0091] The proportion of ingredients for the final product:

[0092] Mesenchymal stem cells, 4×10 7 a / mL;

[0093] Sodium Hyaluronate, 4%;

[0094] Sodium chloride, 0.9%;

[0095] water for injection, the rest.

[0096] Preparation method:

[0097] Obtain mesenchymal stem cells from placenta with reference to the method described in CN102586184A (2012100446386).

[0098] Weigh sodium hyaluronate and sodium chloride, add an appropriate amount of water for injection to dissolve, autoclave at 121°C for 15 minutes, and cool for later use; mix the mesenchymal stem cell suspension with the aqueous solution of sodium hyaluronate and sodium chloride, add water To the full amount, mix evenly, dispense into prefilled syringes, and store in a -80°C refrigerator for frozen storage.

Embodiment 2

[0099] Embodiment 2, the composition of placenta MSC

[0100] The proportion of ingredients for the final product:

[0101] Mesenchymal stem cells, 2×10 7 a / mL;

[0102] Sodium hyaluronate (molecular weight is 400000), 5%;

[0103] Sodium chloride, 0.8%;

[0104] water for injection, the rest.

[0105] Preparation method:

[0106] Obtain mesenchymal stem cells from placenta with reference to the method described in CN102586184A (2012100446386).

[0107] Weigh sodium hyaluronate and sodium chloride, add an appropriate amount of water for injection to dissolve, autoclave at 121°C for 15 minutes, and cool for later use; mix the mesenchymal stem cell suspension with the aqueous solution of sodium hyaluronate and sodium chloride, add water To the full amount, mix evenly, dispense into prefilled syringes, and store in a -80°C refrigerator for frozen storage.

Embodiment 3

[0108] Embodiment 3, the composition of placenta MSC

[0109] The proportion of ingredients for the final product:

[0110] Mesenchymal stem cells, 5×10 7 a / mL;

[0111] Sodium hyaluronate (molecular weight is 1000000), 3%;

[0112] Sodium chloride, 1.0%;

[0113] water for injection, the rest.

[0114] Preparation method:

[0115] Obtain mesenchymal stem cells from placenta with reference to the method described in CN102586184A (2012100446386).

[0116] Weigh sodium hyaluronate and sodium chloride, add an appropriate amount of water for injection to dissolve, autoclave at 121°C for 15 minutes, and cool for later use; mix the mesenchymal stem cell suspension with the aqueous solution of sodium hyaluronate and sodium chloride, add water To the full amount, mix evenly, dispense into prefilled syringes, and store in a -80°C refrigerator for frozen storage.

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Abstract

The invention relates to a method for treating osteoarthritis by stem cells and a composition. Particularly, the invention relates to a cell treatment composition for treating osteoarthritis and cartilage defects. The composition includes mesenchyma stem cells, hyaluronic acid or pharmaceutical salt, sodium chloride and water. The invention also relates to a method for preparing the composition. The cell treatment composition has the excellent properties.

Description

technical field [0001] The present invention belongs to the field of pharmacy, which relates to a pharmaceutical composition for treating and / or preventing acute and / or chronic osteoarthritis. In particular, it relates to methods and compositions for treating osteoarthritis using stem cells. Background technique [0002] Osteoarthritis, the most common form of arthritis, is characterized by a slow degenerative process of articular cartilage, subchondral bone, with marginal osteophyte formation and low-grade inflammation. The chronic form of osteoarthritis is believed to affect 15% of the population. Of these, a quarter were severely disabled. The cause of most cases of osteoarthritis is unknown and is referred to as "primary osteoarthritis". When the cause of osteoarthritis is known, it is called "secondary osteoarthritis". Secondary osteoarthritis is caused by another disease or condition. Conditions that can lead to secondary osteoarthritis include repeated injury or ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K47/36A61P29/00A61P19/02A61P19/04
CPCA61K35/28A61K47/36
Inventor 周丹刘冰尚聪汤乐李晶
Owner BOYALIFE
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