68results about How to "The preparation method is simple and gentle" patented technology

The invention discloses a method for preparing a porous polymer micro needle by using a template method and an application thereof. The method comprises the following steps of: (1) dissolving a polymer and a template agent in a solvent to prepare a polymer solution; (2) filling the polymer solution into a micro needle mould; (3) removing the solvent in the obtained micro needle mould to obtain a solid micro needle; (4) removing the template agent in the obtained solid micro needle to obtain the porous polymer micro needle. By improving the overall process flow design of the preparation method,the method can solve the technical problems of a complex preparation process, harsh conditions, a complicated process, high price, difficult control of the pore structure and size, difficult large-scale production and application and the like of the porous polymer micro needle, the obtained porous polymer micro needle can be used for tissue fluid extraction and transdermal administration, such asskin tissue fluid and blood extraction and transdermal administration of proteins, polypeptides and small molecular drugs in cosmetic, hair growth, immunity, treatment and other applications.

The invention relates to a method for treating osteoarthritis by stem cells and a composition. Particularly, the invention relates to a cell treatment composition for treating osteoarthritis and cartilage defects. The composition includes mesenchyma stem cells, hyaluronic acid or pharmaceutical salt, sodium chloride and water. The invention also relates to a method for preparing the composition. The cell treatment composition has the excellent properties.

The invention provides a method for preparing a titanium dioxide photocatalysis multi-layer film with an interface defect, which belongs to the technical field of titanium dioxide catalysts. On a low-temperature plasma film, a defect is introduced into a TiO2 double-layer film which is already prepared by a technique; by repeating the step for many times, the photocatalysis multi-layer film has the very good photoinduced hydrophilicity of the prepared TiO2 and very good photocatalysis activity and can carry out a photocatalysis reaction effectively. Furthermore, the multi-layer film has wide application range, low cost, convenient use and easy separation and recovery.

The invention relates to a boron nitride nanotube-nano-cellulose fiber composite material. The composite material is prepared from the following materials in percentage by mass: 5 to 40 percent of boron nitride nanotube, and 60 to 95 percent of nano-cellulose fiber. The invention further relates to a preparation method of the boron nitride nanotube-nano-cellulose fiber composite material. The preparation method comprises the following steps: mixing the boron nitride nanotube with a nano-cellulose fiber aqueous solution; performing ultrasonic treatment; performing solid-liquid separation to obtain the boron nitride nanotube-nano-cellulose fiber composite material. By adopting the composite material, the interface thermal resistance and phonon scattering action are reduced effectively, the heat-conducting property of the composite material is improved, and high dimension stability is achieved; the composite material is biodegradable. The preparation method is simple and mild, and can be applied to industrial production.

The invention relates to the field of biology and medicines, and especially relates to an adipose-derived stem cell preparation and a preparation method thereof. The adipose-derived stem cell preparation comprises adipose-derived stem cells, hyaluronic acid, alhumin and normal saline. The adipose-derived stem cells can maintain good survival rate and activity, and do not differentiate under the protection of hyaluronic acid, alhumin and normal saline in the adipose-derived stem cell preparation. The preparation method of the adipose-derived stem cell preparation provided by the invention can avoid damages of the adipose-derived stem cells, and improves the survival rate of the adipose-derived stem cells in the preparation. Experiments show that the adipose-derived stem cell preparation can maintain the survival rate of the adipose-derived stem cells above 99%, and allows the cells to have uniform size and shuttle-shaped form. After the adipose-derived stem cells are preserved at 0-4DEG C for 72h, the cells still maintain good form and have no volume enlargement or other abnormal phenomena, the survival rate still maintains above 95%, and the adipose-derived stem cells still maintain the characteristics of stem cells, and do not differentiate.

The invention provides solid perfume based on a liquid marble and a preparation method thereof and belongs to the field of colloid and interface science and cosmetics. The solid perfume is provided with a structure shown as follows. The method for preparing the solid perfume comprises the steps of taking hydrophobic powder as a stabilizing agent, adding liquid drops above the stabilizing agent dropwise, and preparing the liquid marble, namely the solid perfume, through a rolling dripping method. The preparation process of the long-acting slow-release solid perfume based on the liquid marble is simple and mild, the solid perfume has excellent stability, slow-release performance and gas permeability, can keep the air fresh effectively for a long time and have wide application prospects.

The invention relates to a tumor antigen loaded polydopamine nanoparticle, and a preparation method and an application thereof. The tumor antigen loaded polydopamine nanoparticle is prepared by covalently connecting a tumor antigen to the surface of a polydopamine nanoparticle through Michael addition and Schiff base reaction, wherein the tumor antigen comprises, but is not limited to, one or moreselected from the group consisting of a specific tumor antigen peptide fragment, a tumor cell lysate, a specific tumor antigen DNA fragment and an adjuvant CpG. The tumor antigen loaded polydopaminenanoparticle prepared by using the method provided by the invention has good biocompatibility, can effectively activate in-vivo anti-tumor immunity, significantly inhibits the growth of tumor cells, can improve the tumor immunosuppressive microenvironment, further enhances the anti-tumor activity of the human body, can be used for immune treatment of tumors, and is simple and pollution-free in preparation method.

The invention discloses a method for preparing a porous polymer microneedle based on a phase separation technology and application of the method. The method comprises the following steps that 1, a polymer is dissolved into a solvent A to obtain a polymer solution; 2, the polymer solution is put into a microneedle mould; 3, the microneedle mould is placed in a poor solvent B, so that phase separation of the polymer in the microneedle mould is facilitated; 4, the poor solvent B is removed, and then the porous polymer microneedle can be obtained. Through the simple and mild phase separation technology, the porous polymer microneedles can be prepared on a large scale, and therefore, the technical problems can be solved that the preparation process of the porous polymer microneedle is complicated, the conditions are harsh, the technology is tedious, the price is high, the pore structure, the size and the distribution are difficult to control, and it is difficult for large-scale production and application. The prepared porous polymer microneedle can be used for interstitial fluid extraction and transdermal drug delivery.

The invention relates to a method for a preparing porous titanium dioxide ball. The method comprises the following steps that 1), a titanium dioxide precursor is added into dispersion liquid of an m-aminophenol formaldehyde resin ball, after the mixture is stirred for several hours, centrifugal separation is conducted, and a compound of the m-aminophenol formaldehyde resin ball and the titanium dioxide precursor is obtained; 2), the obtained compound of the m-aminophenol formaldehyde resin ball and the titanium dioxide precursor in the step one is placed in an oven for standing for some time,and thermal treatment is conducted; 3) after thermal treatment, the compound of the m-aminophenol formaldehyde resin ball and the titanium dioxide precursor is placed in a nitrogen atmosphere for hightemperature carbonization and further roasted to remove carbon, and the porous titanium dioxide ball is obtained; compared with the prior art, the preparation method is simple and mild, the size of titanium dioxide holes can be adjusted by adjusting the concentration of the titanium dioxide precursor, the cost is low, the period is shorter, and the yield is higher.

The invention discloses a preparation method of quercetin-loaded nano-selenium. The quercetin-loaded nano-selenium comprises the following raw materials: a reducing agent, a reaction solution and a selenium source, wherein quercetin is used as the reducing agent, sodium selenite (Na2SeO3) is used as the selenium source, the reaction solution contains a stabilizer and a mixed solvent of methanol and water, and the stabilizer is gum arabic (As). The preparation method comprises the following specific steps: preparing nano selenium particles for loading quercetin, firstly preparing the reaction solution containing the stabilizer, adding quercetin and sodium selenite substance into the reaction solution, stirring and reacting for a period of time at room temperature, then adding a proper amount of tween 80, continuously stirring, removing methanol by rotary evaporation, and freeze-drying to obtain the quercetin-loaded nano-selenium. The invention further discloses application of the quercetin-loaded nano-selenium in treating Alzheimer's disease. The invention aims to provide a preparation method and application of a medicament for treating Alzheimer's disease.

The invention discloses a palladium-copper gold nanometer spinous oxygen reduction catalyst and a preparation method thereof. The preparation method comprises the following steps: respectively preparing a sodium tetrachloropalladate solution, a copper chloride solution and a chloroauric acid solution which have the concentration of 1 to 30 mM, a hydrochloric acid solution with the concentration of1 to 10 M and an ascorbic acid solution with the concentration of 0.01 to 0.5 M; mixing the sodium tetrachloropalladate solution, the copper chloride solution and the chloroauric acid solution of which the total volume is 4.5 mL, then adding 0.1 to 0.5 mL of the prepared hydrochloric acid solution, adding 0.1 to 0.5 g of potassium bromide and F127, and mixing uniformly; finally adding 1 to 10 mLof the ascorbic acid solution; after the solutions are fully mixed, placing the mixture in an oil bath pan, heating to 70 to 130 DEG C, reacting for 0.1 to 3 hours, and performing washing, centrifuging and drying to obtain the palladium-copper gold nanometer spinous electrocatalyst. The preparation method has the advantages of simple preparation process, and short reaction time. The material prepared at normal temperature and under normal pressure has superior electrochemical oxidation reduction performance.

The invention aims to solve the problem about the anticancer specificity of a PEGylated dendrimer drug delivery system and provides a preparation method of a GFLG-based PEGylated peptide dendrimer drug delivery system. The preparation method is characterized in that the drug delivery system is a conjugate of the PEGylated peptide dendrimer, a targeted functionality factor GFLG and an anticancer therapeutic factor; GFLG is adopted to connect the therapeutic factor and the PEGylated peptide dendrimer to form a functionality dendrimer, so that excellent biocompatibility is obtained, and the remarkable anticancer curative effect is achieved by using medicine of the same or lower dosage.

A boron-doped silver nano spongy catalyst for electrochemical ammonia synthesis is prepared by the following method: preparing an N,N-dimethylformamide (DMF) solution and aqueous solution of silver nitrate with a concentration of 0.01-1.0 M, and preparing a DMF solution and aqueous solution of sodium borohydride with a concentration of 0.01-1.0 M; adding 1.0 mL of the DMF solution and aqueous solution of silver nitrate with the concentration of 0.01-1.0 M into 5.0 mL of the DMF solution and aqueous solution of sodium borohydride with the concentration of 0.01-1.0 M, separately carrying out stirring for reacting for 1.5-2.5 hours under an ice-water bath condition and under a normal temperature condition, and carrying out washing, centrifuging and drying to obtain the boron-doped silver nanospongy catalyst. The invention also provides a preparation method of the boron-doped silver nano spongy for electrochemical ammonia synthesis. The preparation process is simple, the reaction time isshort, and the prepared material has excellent electrochemical nitrogen reduction performance at low temperature and normal pressure.

The invention relates to a gold-copper-boron porous network structure electrocatalyst. The gold-copper-boron porous network structure electrocatalyst is produced through a method which comprises the following steps: (1) producing N,N-dimethylformamide solutions, with concentrations of 1-40 mM, of copper chloride and chloroauric acid respectively; (2) taking the N,N-dimethylformamide solutions of the copper chloride and the chloroauric acid respectively according to the total volume of 4 mL for mixing, and then under conditions of room temperature and intense stirring, adding 20 mL of a fresh N,N-dimethylformamide solution, with a concentration of 1-40 mM, of sodium borohydride; and (3) after a reaction is carried out for 1-60 min, conducting washing, centrifugation and drying to obtain thegold-copper-boron porous network structure electrocatalyst. The production method of the gold-copper-boron porous network structure electrocatalyst is further provided. The production method is simple, the reaction time is short, the reaction conditions are mild, and the produced material has excellent performance of electrochemical ammonia synthesis through nitrogen reduction.