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Preparation of quercetin-loaded nano-selenium and application of quercetin-loaded nano-selenium in treating Alzheimer's disease

A technology of quercetin and nano-selenium, which is applied in the field of biomedicine, can solve the problems of high utilization rate of organic selenium, fast absorption of inorganic selenium, poor safety, etc., and achieve improved bioavailability, simple and gentle preparation method, and uniform size Effect

Inactive Publication Date: 2020-01-10
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Selenium exists in nature and can be roughly divided into organic selenium and inorganic selenium according to its combination form. Both of these two types can be absorbed and utilized by organisms, but organic selenium has high utilization rate and slow absorption, while inorganic selenium has fast absorption and poor safety. Toxicity

Method used

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  • Preparation of quercetin-loaded nano-selenium and application of quercetin-loaded nano-selenium in treating Alzheimer's disease
  • Preparation of quercetin-loaded nano-selenium and application of quercetin-loaded nano-selenium in treating Alzheimer's disease
  • Preparation of quercetin-loaded nano-selenium and application of quercetin-loaded nano-selenium in treating Alzheimer's disease

Examples

Experimental program
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Effect test

Embodiment 1

[0031] The present embodiment provides a quercetin-loaded nano-selenium, the quercetin-loaded nano-selenium includes the following raw materials in proportion according to the specified parts: reducing agent, reaction solution and selenium source, and the quercetin is used as A reducing agent; the sodium selenite is used as a selenium source, and the reaction solution is a reaction solution containing a stabilizer, wherein the stabilizer is gum arabic, and the reaction solution is a mixed solvent of methanol and water.

[0032]Further, the selenium source can also be one or more of selenium oxide, selenium trioxide, and selenous acid.

[0033] Further, the overall size of the quercetin-loaded nano-selenium is 60nm-120nm, and the potential is -10mv.

[0034] Further, the application of the quercetin-loaded nano-selenium in the treatment of Alzheimer's disease.

[0035] This embodiment also discloses a preparation method of nano selenium loaded with quercetin, which comprises t...

Embodiment 2

[0047] A kind of product disclosed in the present embodiment is the product in the prior art, and as the contrast sample of embodiment 1, its preparation method comprises the steps:

[0048] Dissolve 0.004g of gum arabic in 0.4ml of secondary water, add 0.1ml of 20mM sodium selenite solution prepared with secondary water, stir at room temperature for 20min; add 1.2ml of 10mM citric acid solution prepared with secondary water , add 0.3ml of secondary water and stir at room temperature for 5h, then add 3.6mg of Tween 80 and incubate at room temperature for 1h, freeze-dry to obtain the final product.

[0049] The prepared selenium nanoparticles loaded with citric acid were subjected to a potential test, and the potential of the obtained selenium nanoparticles loaded with citric acid was -10mv.

[0050] The resulting product of this example is photographed with a scanning electron microscope, and the results are shown in figure 2 , the average diameter of nanoparticles is 120nm....

Embodiment 3

[0052] Then Example 1 and Example 2 are compared, and the experimental method is as follows: a kind of selenium nanoparticles loaded with quercetin disclosed in Example 1 is used for the research of treating Alzheimer's disease, based on Alzheimer's disease Among them, the pathogenesis of β-amyloid protein, the material created and prepared by the present invention is used to inhibit Abeta 1-42 For the test of monomer aggregation, the experimental method is as follows:

[0053] 1) In Abeta 1-42 Add hexafluoroisopropanol to the protein to form a 1mg / ml solution, seal and sonicate for 30min, let stand for 24h, then centrifuge at 13000r / min for 30min, take the supernatant and freeze-dry to obtain Abeta 1-42 Dissolve the membrane with 5mM sodium hydroxide, centrifuge at 13000r / min for 30min, take the supernatant to test Abeta 1-42 Concentration and secondary structure, get Abeta 1-42 stock solution;

[0054] 2) Take a certain amount of Abeta 1-42 The stock solution was neutra...

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Abstract

The invention discloses a preparation method of quercetin-loaded nano-selenium. The quercetin-loaded nano-selenium comprises the following raw materials: a reducing agent, a reaction solution and a selenium source, wherein quercetin is used as the reducing agent, sodium selenite (Na2SeO3) is used as the selenium source, the reaction solution contains a stabilizer and a mixed solvent of methanol and water, and the stabilizer is gum arabic (As). The preparation method comprises the following specific steps: preparing nano selenium particles for loading quercetin, firstly preparing the reaction solution containing the stabilizer, adding quercetin and sodium selenite substance into the reaction solution, stirring and reacting for a period of time at room temperature, then adding a proper amount of tween 80, continuously stirring, removing methanol by rotary evaporation, and freeze-drying to obtain the quercetin-loaded nano-selenium. The invention further discloses application of the quercetin-loaded nano-selenium in treating Alzheimer's disease. The invention aims to provide a preparation method and application of a medicament for treating Alzheimer's disease.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to nano selenium loaded with quercetin, a preparation method thereof and an application thereof in treating Alzheimer's disease. Background technique [0002] Alzheimer's disease (AD), also known as senile dementia, is an insidious progressive neurodegenerative disease, and is the most common type of senile dementia. The main manifestations of AD are neuropsychiatric symptoms such as progressive memory impairment, cognitive dysfunction, personality changes, and language barriers, which seriously affect social, occupational, and life functions. The characteristic pathological changes of AD are extracellular senile plaques formed by the deposition of β-amyloid protein, neurofibrillary tangles formed by hyperphosphorylation of tau protein, and neuron loss with glial cell proliferation. At present, with the increase of the aging population, the number of people suffering from the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/04A61K31/352A61K47/36A61K47/02A61P25/28A61P39/06
CPCA61K31/352A61K33/04A61K47/02A61K47/36A61P25/28A61P39/06A61K2300/00
Inventor 张翼齐钰洁彭伟军胡先哲李强宋显温郑俊
Owner CENT SOUTH UNIV
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