30results about How to "Gentle process" patented technology

This invention belongs to the field of cosmetic technology, specifically relating to a method for preparing a lemon fruit extract containing sennaol and its application in soothing. This invention provides a method for preparing a lemon fruit extract containing sennaol, utilizing the strong penetration and dissolving power of a green solvent and the high selectivity of enzymatic reactions to achieve not only efficient extraction and conversion of sennaol but also naturally endowing the final product with excellent water solubility and chemical stability. The prepared lemon fruit extract can be directly applied to water-based formulations without complex modification or the addition of large amounts of co-solvents, maintaining potent skin-soothing activity. The entire process is gentle and safe, with no irritating organic solvents or strong acid residues, making it particularly suitable for cosmetic development targeting sensitive skin.

This invention discloses a method for processing high-transparency and high-colorfastness wool cellulose fiber segment-dyed and spray-dyed yarn using multi-enzyme synergistic pretreatment and its application. The processing method includes: yarn pretreatment, keratinase pretreatment, synergistic treatment of keratinase and protease, cellulase treatment, synergistic inactivation of multiple enzymes and pH control, preparation of anionic pigment paste, segment-dyed spray-dyeing, and drying and curing fixation. The synergistic pretreatment of keratinase, keratinase, protease, and cellulase forms a technical chain of barrier breaking, loosening, chain reduction, and coarsening, resulting in a dye penetration index of >80% for the pigment spraying. The pH-controlled wool fibers carry a strong positive charge, which strongly electrostatically adsorbs with the anionic pigment paste, achieving a dry rubbing colorfastness of 4-5 and a wet rubbing colorfastness of 4. The fiber strength loss is small, and the method is environmentally friendly. It is suitable for segment-dyed and spray-dyed yarns of pure wool and blended yarns of wool with cellulose fibers such as cotton, viscose, Tencel, and Modal, and can be widely used in home textiles, clothing, and other fields.

This invention relates to the field of electronic packaging composite materials technology, and particularly to a thermally conductive epoxy encapsulation material, its preparation method, and its application. The invention uses Fe3O4 nanospheres and sodium polyacrylate solution as raw materials to prepare polyacrylic acid-modified Fe3O4 nanospheres; these are then blended with adipic acid and epoxy resin, and a thermally conductive epoxy encapsulation material is prepared through a curing reaction. The thermally conductive epoxy encapsulation material prepared by the method of this invention exhibits uniform dispersion of Fe3O4 nanospheres, good interfacial compatibility, and high thermal conductivity, demonstrating excellent thermal conductivity, interfacial reliability, and mechanical properties, making it well-suited for electronic chip packaging applications.

The invention discloses a construction method of a colorectal tumor model based on 3D printing, which comprises the following steps: dissolving methacrylated gelatin and phenyl (2, 4, 6-trimethylbenzoyl) lithium phosphate together to obtain a mixed solution, adding colorectal cancer cells HCT-116 and human skin fibroblasts BJ into the mixed solution, and uniformly mixing to obtain bio-ink; and loading the bio-ink into a charging barrel of an extrusion type biological 3D printer for 3D printing to obtain the tumor cell-carrying gel microspheres, and culturing in vitro to obtain the tumor cell-carrying gel microspheres. According to the method, the upper limit of cell density of liquid drop biological 3D printing is broken through, stable printing of biological ink with the cell density as high as 1 * 10 < 8 > cells / mL is successfully achieved through optimization of a rheological window, the density is close to the physiological level of solid tumors, the printed tumor model can truly simulate the high compactness of the tumors and the nutrition / oxygen gradient effect caused by the high compactness of the tumors, and the tumor model has good application prospects. And an experimental platform closer to the in vivo is provided for researching the microenvironment heterogeneity in the tumor.

The invention discloses a direct high-temperature regeneration method of a waste lithium iron phosphate positive plate in a water vapor atmosphere. The method comprises the following steps: directly placing the positive plate obtained by discharging and disassembling the waste lithium iron phosphate battery into a reaction container, directly regenerating at 600-650 DEG C by taking superheated steam as a gasification medium, and removing aluminum foil after the regeneration process is completed, thereby obtaining the regenerated lithium iron phosphate positive plate. According to the method provided by the invention, the structure of lithium iron phosphate can be completely repaired at a relatively low temperature, and aluminum foil fusion is avoided. Compared with a traditional recycling process, the method has the advantages that complex pretreatment steps such as smashing, screening and stripping are omitted, material loss is reduced, the whole process is mild and environmentally friendly, and the method is short in process and easy and convenient to operate.

The invention discloses an external nursing cream containing white muscardine silkworm and gentiana macrophylla and a preparation method thereof, the nursing cream takes white muscardine silkworm and gentiana macrophylla as main raw materials, the main raw materials are extracted by ethanol with the concentration of 20-99%, and the ethanol can be edible wine, health care medicinal liquor or medical ethanol; and the finished product is mild for external use after being blended by a matrix, and the irritation is controllable. Auxiliary materials such as artificial musk, ground beeltle, mirabilite, myrrh and angelica sinensis can be selectively added, and the ointment is prepared by extracting, concentrating and adding an external matrix. The invention has the advantages of simple and mild formula, flexible and simple process, easily available raw materials and wide application range, and is suitable for daily external skin care.

This invention belongs to the field of biological engineering technology, specifically relating to an MXene-based photothermal coupling composite film for bone repair and its preparation method; comprising MXene, type I collagen, and gelatin solution, obtained by sequential bridging and cross-linking with FeCl3 solution; wherein the mass ratio of MXene to type I collagen is (3-9):(1-7), and gelatin accounts for 3.6%-10.4% of the final composite material mass; the preparation method includes: ultrasonically dispersing MXene and mixing it with gelatin solution, then compounding it with type I collagen solution, vacuum filtering and drying to form a matrix film, and finally cross-linking it by impregnation with FeCl3 solution; this film has mechanical properties matching natural bone tissue, good biocompatibility, and high-efficiency photothermal conversion capability under near-infrared light irradiation, and is suitable for bone defect repair and photothermal synergistic therapy.

The invention relates to the technical field of chemical synthesis, in particular to a preparation method of a key intermediate of trifluorobenzene pyrimidine. The key intermediate is N-(5-pyrimidinylmethyl)-2-aminopyridine, and the preparation method comprises the following steps: mixing 2-fluoropyridine and ammonia water, heating to react, after the reaction is finished, naturally cooling to room temperature, and slowly releasing residual ammonia gas in a reaction container; and adding acetonitrile and 5-bromomethyl pyrimidine, heating, stirring to react, cooling to normal temperature, keeping the temperature to react for 1 hour, cooling to-5 DEG C, stirring for 2 hours, filtering and drying to obtain the N-(5-pyrimidinylmethyl)-2-aminopyridine. The target product is obtained by taking 2-fluoropyridine as a starting raw material through one-pot substitution, and the route has the advantages of easily available raw materials, controllable cost, simple and easy operation, easily available raw materials, low cost, mild conditions, mature process, high yield and purity, and is suitable for industrial mass production.

The invention provides an acellular matrix fiber woven scaffold as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The acellular matrix fiber woven scaffold comprises a woven structure unit, wherein the woven structure unit comprises warp yarns and a plurality of acellular matrix fiber weft yarns; the acellular matrix fiber is prepared by cutting an acellular matrix paper-based material at equal intervals; the plurality of acellular matrix fiber wefts are arranged in parallel according to a bionic trend and are interwoven and fixed through the warps, so that a bionic pattern simulating natural tissue fiber arrangement is formed in the weaving structure unit. The acellular matrix fiber scaffold is prepared by innovatively applying a weaving technology, the scaffold can improve the cell loading efficiency, is beneficial to self-repair and reconstruction of damaged tissues, provides suitable survival and growth conditions for cells, and has good application prospects in the field of biomedical engineering, especially in the aspects of tissue repair and regenerative medicine. And huge application potential is shown.

This invention provides a method for preparing a vanadium-titanium catalyst repair agent and a method for repairing vanadium-titanium catalysts using the agent. The preparation method of the repair agent is as follows: A seed mother liquor from the Bayer process for alumina production is added to lime slurry and stirred to obtain a filter cake and filtrate; the filter cake is added to a mixed solution of organic acid and ammonium salt and stirred to obtain an active component replenishment solution; aluminum hydroxide slurry from the Bayer process for alumina production is added to a citric acid solution and stirred to obtain an aluminum sol strength repair solution; the active component replenishment solution and the aluminum sol strength repair solution are then mixed to obtain the final product. The repair method is as follows: the vanadium-titanium catalyst to be repaired is immersed in the repair agent, dried, and calcined to obtain the final product. This invention cleverly incorporates the Bayer process to obtain the key materials required for the repair process, thereby achieving the repair of vanadium-titanium catalysts; after repair, the radial and axial compressive strength, wear resistance, and catalytic activity of the catalyst are all significantly improved.

This invention discloses a mica nanosheet and its preparation method, belonging to the field of two-dimensional nanofiller technology. The preparation method disclosed in this invention involves heat-treating bulk mica, followed by purification in an aqueous nitric acid solution to obtain pretreated mica powder; dispersing the pretreated mica powder in a solvent to obtain a mixed slurry; adding an oxidant to the mixed slurry and stirring the mixture, then adding a small-molecule lithium salt to further react, obtaining a pretreated slurry; adding grinding beads to the pretreated slurry for sand milling to achieve exfoliation; subsequently releasing the exfoliated slurry and performing post-processing to obtain mica nanosheets. This method solves the technical problem of simultaneously achieving efficient, green, and controllable exfoliation, and possesses scalability potential, which is difficult to achieve with existing methods.

The invention discloses high-specific-surface-area porous carbon based on coal gasification carbon residues and a preparation method and application thereof.The method comprises the steps that coal gasification residues serve as raw materials and are treated to obtain a carbon-rich precursor, and the carbon-rich precursor is carbonized to obtain a coal gasification residue-based hard carbon matrix; then, the hard carbon matrix is in contact with a modified solution containing hydrogen peroxide, synergistic pore-forming agents such as an inorganic gas generating type pore-forming agent, an organic small molecule pore-forming agent and a soft and hard template pore-forming agent can be further added into the modified solution, and selective oxidation etching and gas-phase pore-forming are carried out under the conditions of controlled temperature, time and pH; a multi-scale micropore and mesopore structure is formed; and washing, drying and re-carbonizing or thermally annealing to obtain the coal gasification slag-based hard carbon material with high specific surface area. Compared with unmodified hard carbon, the method has the advantages that the specific surface area can be obviously increased, micropores and mesopores are synergistically developed, electrolyte infiltration and an ion transmission channel are obviously improved, and controllable construction of a multistage pore channel can be realized on the premise of ensuring relatively high yield and structural stability.

This invention relates to the field of medical materials technology, specifically to recombinant humanized collagen composite microspheres, their preparation method, and applications. This invention provides a preparation method that requires no chemical reagents and involves extremely simple steps. The method employs a purely physical process of "ultrasonic dispersion + liquid nitrogen rapid freezing + freeze-drying," which simplifies the operation process, lowers equipment requirements, and reduces production error-tolerant costs, thereby achieving efficient preparation and stable mass production of composite microspheres. These composite microspheres have broad application prospects in soft tissue repair, especially in minimally invasive cosmetic procedures and complex wound filling.

The invention discloses a method for preparing ethanol through detoxification-free fermentation of furfural residues and application. The method comprises the following steps: S1, carrying out high-solid-content enzymolysis on acidic furfural residues which are not subjected to any detoxification pretreatment to obtain furfural residue enzymatic hydrolysate; s2, liquefying and saccharifying the waste starch to obtain a starch saccharification liquid; and S3, mixing the furfural residue enzymatic hydrolysate and the starch saccharification liquid in proportion, inoculating zymomonas mobilis, and carrying out co-fermentation to produce ethanol. According to the method, key technologies such as detoxification-free treatment, high-solid-content enzymolysis and co-fermentation with starch saccharification liquid are combined, and the steps of neutralization, water washing and deep detoxification which are necessary in a traditional process are thoroughly abandoned, so that the process is greatly simplified, water resources are saved, the treatment cost is reduced, and the problem of toxicity of inhibitors in raw materials is effectively solved. The method provides an efficient, economical and environment-friendly brand-new way for realizing high-valued resource utilization of the furfural residues and the waste starch.

The application belongs to the technical field of oilfield exploitation, and particularly relates to a foam stabilizer based on graded modification of Enteromorpha, and a preparation method and application thereof. The foam stabilizer comprises a foam stabilizer or a chelated foam stabilizer. The preparation method of the foam stabilizer comprises the following steps: step one, Enteromorpha pretreatment: washing and drying Enteromorpha, and then crushing; step two, hot water leaching separation: adding deionized water according to a solid-liquid ratio of 1:25-35, stirring and leaching at 85-95 DEG C for 4 h, and filtering to obtain filtrate and residue; and step three, preparation of the foam stabilizer or the chelated foam stabilizer: drying the residue at 80 DEG C for 24 h, and sieving through a 200-mesh sieve to obtain the foam stabilizer; and alkali-activating the foam stabilizer and then performing a carboxylation reaction to obtain the chelated foam stabilizer. The application realizes high-value utilization of waste, and has the advantages of mild process, low cost, excellent foam stabilization under high temperature and high salinity, and effectively makes up for the defects of the prior art.

The application belongs to the technical field of high-pressure hydrogen storage cylinders and interface engineering, and discloses a self-repairing anti-fatigue hydrogen barrier coating and application thereof in a type IV hydrogen storage cylinder. The self-repairing anti-fatigue hydrogen barrier coating is formed by curing a water-based polyurethane adhesive. The water-based polyurethane adhesive comprises water, a water-based polyurethane dispersion, a dihydrazine crosslinking agent, a flexible polyol, and a low-surface-energy filler. The application is as follows: first, the outer surface of a thermoplastic polymer liner is activated to introduce polar groups; then, the self-repairing anti-fatigue hydrogen barrier coating is formed on the activated surface by spraying; and finally, the outer surface of the self-repairing anti-fatigue hydrogen barrier coating is wrapped with a carbon fiber reinforced resin and cured to obtain the type IV hydrogen storage cylinder. The application improves the interface fatigue life from the structural level without significantly increasing the weight, wall thickness, and manufacturing complexity of the type IV high-pressure hydrogen storage cylinder, and avoids the problem that it is difficult to simultaneously consider hydrogen barrier, toughness, and self-healing.

The application discloses a preparation method of a drug sustained-release hydrogel support and a three-dimensional osteogenic induction application of hiPSCs. Firstly, a CHIR99021@BSA-DEX@PLGA time-sequential double sustained-release composite microsphere of a core-shell structure is prepared, then the freeze-dried GelMA powder is dissolved in a PBS solution containing a photoinitiator LAP, the CHIR99021@BSA-DEX@PLGA time-sequential double sustained-release composite microsphere is added after stirring and cooling, and a drug-loaded hydrogel precursor is obtained; the precursor is injected into a mold and irradiated by a specific wavelength light source to initiate free radical polymerization of a methacryl group into glue, and a drug-loaded GelMA porous hydrogel support is obtained after demolding, freezing and drying; the drug-loaded GelMA porous support is covalently grafted with VN polypeptide through EDC / NHS chemical coupling to obtain a VN-GelMA drug-loaded porous hydrogel support. The application simulates the signal time sequence regulation law in the embryonic bone formation process and significantly improves the osteogenic differentiation efficiency and mineralization quality by constructing a biomimetic three-dimensional osteogenic microenvironment. The VN polypeptide enhances the specific recognition of stem cells and significantly improves the three-dimensional cell compatibility. The application can reduce the dependence on exogenous induction factors and is safe, low in cost and stable in induction.

The invention discloses an application of an ointment taking a banana peel extract as an effective component in prevention and treatment of beriberi. The banana peel extract is prepared by extracting fresh banana peel, has a remarkable inhibition effect on trichophyton rubrum which is a main pathogenic bacterium of beriberi, and can achieve the beneficial effects of sterilizing, relieving itching and preventing keratosis and desquamation after being applied. The banana peel extract is creatively applied to beriberi prevention and treatment, has the characteristics of quick action, remarkable effect, mild process and no toxic or side effect, and provides an effective way for beriberi prevention and treatment.

The application discloses a multifunctional polyvinyl alcohol-based hydrogel and a preparation method and application thereof, and belongs to the technical field of high polymer hydrogel material preparation.The hydrogel takes polyvinyl alcohol (PVA) as a base material, takes polydopamine-coated sea squirt nanocellulose (PDA@TCNCs), beta-glycerophosphate sodium (GP) and phytic acid (PA) as functional components; the mass-volume ratio of PVA, PDA@TCNCs, GP and PA aqueous solutions is 2.62g:0.05g-0.25g:1.0g:4mL-12mL.The hydrogel has excellent mechanical properties, anti-swelling properties and antibacterial properties, and has good biocompatibility, and has wide application prospects in wearable strain sensors, underwater motion monitoring and triboelectric nanogenerators and other fields.

The invention relates to the technical field of preparation of camellia oil, and particularly discloses health-care direct-drinking camellia oil and a preparation method thereof, and the health-care direct-drinking camellia oil comprises the following components in parts by weight: 100-200 parts of camellia oil fruits; 3-6 parts of carclazyte; 5-10 parts of bamboo charcoal activated carbon; and 9-18 parts of water. According to the health-care direct-drinking camellia oil and the preparation method thereof, an advanced biotechnology is assisted, just harvested mature camellia fruits are subjected to direct ultrasonic treatment, drying and low-temperature squeezing to obtain squeezed camellia oil, then two low-temperature decoloring and three physical degumming procedures are performed, 92.3% of pigments and 96.8% of colloid components in the camellia oil are removed, and the health-care direct-drinking camellia oil is obtained. The almost colorless and transparent direct-drinking camellia oil product is obtained, the viscosity and the greasy feeling are remarkably reduced, active substances such as triterpenoid saponin and squalene are reserved to the maximum extent in the product, and the prepared direct-drinking oil is almost aqueous, low in greasy feeling, light and refreshing in taste, rich in throat-clearing and intestine-moistening functional substances and suitable for being eaten by people. The effects of diminishing inflammation of throats of middle-aged and elderly people, relieving constipation and digestive system development of infants are particularly good.

The invention belongs to the technical field of polymer solid-state batteries, and particularly relates to a preparation method and application of a flame-retardant N and S-containing in-situ polymerization gel electrolyte. The gel electrolyte is composed of a diolefin chain extender, a disulfydryl chain extender, a trifunctional isocyanurate-containing cross-linking agent, a catalyst, an organic electrolyte and a lithium salt, and in-situ polymerization is carried out in the battery through a sulfydryl-alkene click chemical reaction to form a three-dimensional network structure. Wherein the isocyanurate unit can improve the interfacial compatibility of the electrolyte and the electrode and the cycling stability of the battery. The electrolyte has the advantages of high ionic conductivity, low interface impedance, excellent thermal stability, incombustibility and the like, is suitable for lithium primary batteries and lithium secondary batteries, and is particularly suitable for in-situ polymerization lithium metal batteries with high requirements on safety. The method is simple and convenient in process, mild in reaction condition, highly compatible with an existing liquid battery manufacturing process, and beneficial to realizing large-scale industrial application.

The application provides a preparation method of a high-stability hydrogenation catalyst of an anti-carbon-deposition organic liquid hydrogen storage material, in which active metal Ni and rare earth metal oxide additives are loaded on a carrier by a coprecipitation method to obtain the hydrogenation catalyst. The hydrogenation catalyst prepared by the method has high specific surface area carriers, which can enhance the adsorption capacity of the catalyst and the dispersity of the active metal components, effectively improve the catalytic performance, and simultaneously, the introduction of the rare earth metal oxide modifier can optimize the reaction path, form oxygen vacancies and improve the migration rate of lattice oxygen, effectively inhibit the formation of carbon deposition, and significantly enhance the anti-carbon-deposition performance and cycle stability of the catalyst. The preparation method of the hydrogenation catalyst is mild in process, simple in required equipment, and can be used for large-scale production. In addition, the hydrogenation catalyst has high activity, stability and cycle performance without using noble metals, has good economic benefits, and has a wide popularization and application prospect.

This disclosure relates to the field of pharmaceutical excipients and additives, specifically a method for preparing a pharmaceutical sweetener. The method includes the following steps: preparing modified microalgae polysaccharides; adding the modified microalgae polysaccharides, a first sweetener, a second sweetener, dietary fiber, and an acidity regulator to water, mixing thoroughly, and then spray-drying to obtain the pharmaceutical sweetener. This disclosure solves the problem of poor palatability of microalgae polysaccharides; enabling microalgae polysaccharides, as a filler sweetener, to be compounded with high-intensity sweeteners, other filler sweeteners, dietary fiber, and acidity regulators, thereby possessing both good tableting performance and antioxidant activity.

The invention provides microorganism mineralized andesite powder as well as a preparation method and application thereof. The preparation method comprises the following steps: crushing, screening and grinding blocky andesite to prepare andesite powder; the method comprises the following steps: culturing bacillus pasteurii to obtain thalli and preparing the thalli into a bacterial solution; mixing the andesite powder, the bacterial liquid and urea to construct a microorganism-induced calcium carbonate deposition reaction system; carrying out an oscillating reaction on the reaction system, carrying out solid-liquid separation after the reaction is finished, and collecting a solid precipitate; drying and dispersing the solid precipitate to obtain microorganism mineralized andesite powder; according to the invention, the surface chemical activity and interfacial compatibility of andesite powder particles can be improved, the compactness and mechanical properties of the slurry are enhanced, and the process is green and controllable.

This disclosure relates to a method for oxidizing graphite and its uses. The method includes: mixing graphite, concentrated acid, and potassium permanganate, heating and reacting to obtain a mixture; performing solid-liquid separation on the mixture to obtain graphite oxide and filtrate waste liquid; and refluxing the mixture of filtrate waste liquid and hydrogen peroxide in an open system to separate and extract the solid phase material to obtain manganese oxide. The mass ratio of hydrogen peroxide, potassium permanganate, and graphite is (50~500):(1~5):1, and the mass concentration of hydrogen peroxide is 0.01~2%. This method achieves the simultaneous production of manganese oxide during graphite oxidation without the need for an additional manganese source, simplifying the process. Furthermore, the reflux treatment in an open system provides mild reaction conditions and a simple process flow, effectively reducing the preparation cost of manganese oxide. Simultaneously, this method can significantly reduce the content of manganese ions and other pollutants in wastewater, solving the problem of environmental pollution caused by wastewater discharge during graphite oxidation.

The application discloses a kind of LLZTO / PVDF composite solid electrolyte membrane modified by surface organic layer and preparation method and application thereof, the LLZTO / PVDF composite solid electrolyte membrane is made of LLZTO, PVDF and lithium salt, the lithium salt includes LiTFSI and LiDFOB;The surface of the LLZTO is formed with organic modification layer;The organic modification layer at least includes the passivation layer formed by the reaction of GA and LLZTO surface residual basic component, and the polyether type flexible conductive layer formed by DOL at interface.This application uses LLZTO as inorganic filler, PVDF as polymer matrix, uses LiTFSI and LiDFOB as lithium salt, by introducing GA and DOL to the surface of LLZTO in situ organic layer modification, to inhibit its basicity induced decomposition to PVDF, improve inorganic / organic interface compatibility, and improve the mechanical properties and ionic conduction performance of composite membrane.

The invention discloses a carbon cloth-based composite material, a preparation method and application in a supercapacitor, and belongs to the technical field of supercapacitor electrode materials, and the method comprises the following steps: dissolving nickel nitrate hexahydrate, copper nitrate pentahydrate and an organic ligand containing carboxyl and amino in N-methyl pyrrolidone, adjusting the pH value to 6.07.0, and stirring to form a uniform mixed solution; the preparation method comprises the following steps: carrying out ultrasonic and microwave synergistic activation on pretreated carbon cloth, immersing the carbon cloth in a mixed solution for gradient heating solvothermal reaction to obtain a flexible Ni-Cu bimetal MOF / carbon cloth composite material, immersing the flexible Ni-Cu bimetal MOF / carbon cloth composite material in a composite vulcanizing agent solution prepared from thioacetamide and potassium sulfide, and sequentially carrying out segmented water bath vulcanization and gradient calcination treatment to obtain the flexible Ni-Cu bimetal MOF / carbon cloth composite material. The carbon cloth-based composite material is obtained. The flexible core-shell porous sulfide / carbon cloth composite material with high specific surface area, high specific capacitance, excellent cycle stability and low internal resistance is prepared, the process is mild, the solvent can be recycled, and the composite material has good industrial economy and environmental friendliness.

The invention provides a preparation method of a large-area ultrathin perovskite single crystal. The preparation method comprises the following steps: S1, obtaining a perovskite seed crystal; s2, putting the perovskite seed crystal in the step S1 into a first solvent, and heating to carry out heat treatment on the perovskite seed crystal to change a surface microstructure; s3, placing the treated perovskite seed crystal in a glass container, sequentially adding a second solvent and a perovskite precursor growth solution, and placing the glass container on a heating stage to obtain a perovskite single crystal with the thickness of 1-2cm; s4, fixing the perovskite single crystal on a glass sheet by using paraffin, cutting off seed crystals in the perovskite single crystal by using a blade, and thinning the perovskite single crystal to obtain a large-area ultrathin perovskite single crystal wafer; wherein the second solvent in the step S3 is immiscible with the perovskite precursor growth solution, and the density of the second solvent is smaller than that of the perovskite precursor growth solution. The method has the advantages of inhibiting the growth of the crystal in the vertical direction, reducing the carrier recombination rate and reducing the crack and breakage probability.

This invention belongs to the field of microbial cell lysis and extraction technology, and discloses a highly permeable cationic surfactant microbial lysis buffer and its preparation method. The lysis buffer employs a novel branched alkyl quaternary ammonium salt ester structure, introducing an α-hydroxy ester bond specifically recognized by extracellular lipase at the β-position of the quaternary ammonium nitrogen and the alkyl chain, achieving biodegradability after lysis. The preparation method employs a two-stage sequential lysis process: the first stage utilizes chelating agent microcapsules for slow release in a specific pH environment to pretreat the bacterial cells; subsequently, a stepwise surfactant addition strategy is used to activate and enhance cell membrane permeability; finally, efficient lysis is completed through lipase-catalyzed hydrolysis. This lysis buffer exhibits rapid penetration, high efficiency, targeted and controllable processing, and is environmentally friendly, suitable for the efficient extraction and downstream purification of bacterial proteins, enzymes, polysaccharides, and other intracellular products.

The invention discloses a low-resistance metal semiconductor contact structure for a germanium-based device, a preparation method of the low-resistance metal semiconductor contact structure and the germanium-based device. The low-resistance metal semiconductor contact structure comprises a germanium-doped layer, a graphene layer and a metal electrode which are sequentially stacked, the germanium doping concentration in the germanium doping layer is 1 * 10 < 19 > cm <-3 > to 5 * 10 < 19 > cm <-3 >; the graphene layer at least comprises a layer of graphene crystals; the upper surface of the germanium-doped layer is a stable hydrogen terminal surface, and the graphene layer is in contact with the hydrogen terminal surface through Van der Waals' force; the low-resistance metal semiconductor contact structure comprises an electrode region and a non-electrode region, and the metal electrode is arranged in the electrode region. Graphene is introduced as an atomic-scale flat Van der Waals contact interface layer, so that low-resistance contact between metal and a semiconductor of a germanium-based device is realized, and the overall performance of the device is further optimized.