Method for preparing probiotics microcapsule and its application

A technology of probiotics and microcapsules, which is applied in the field of preparation of probiotic microcapsules, can solve the problems of lactic acid bacteria cell damage, instability between batches, and slow formation of microcapsules, so as to improve survival ability, biological activity, and professional The effect of low quality requirements

Inactive Publication Date: 2011-11-02
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In comparison, the spray drying temperature is too high, the microcapsule formation speed is slow during the extrusion method, and the batches are unstable, whi

Method used

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  • Method for preparing probiotics microcapsule and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Lactobacillus casei and its application

[0038] Lactobacillus casei (Lactobacillus casei) CCTCC NO: M 2010163, this strain has a high removal efficiency of algae toxins, and can effectively remove cholesterol. The strain is used in the field of health food, and has the effects of inhibiting the growth of harmful microorganisms, improving human immunity, and assisting nutrient digestion. See patent application 201010238658.8 for details.

[0039] Taking CCTCC NO: M 2010163 as the starting strain, it was cultured in the seed medium to the logarithmic growth phase, centrifuged and washed to obtain the pure strain, and the prepared sterile sodium alginate (2%, w / v) solution was combined with Modified starch (2%, w / v) is mixed thoroughly in equal volume, and the probiotic concentration after mixing is 10 9 cfu / mL. The mixed liquid is sprayed into CaCl through the spray nozzle 2 (3%, w / v) in solution, Ca 2+ Cross-linking with sodium alginate to form microcapsules. Af...

Embodiment 2

[0047] Take CCTCC NO: M 2010163 as the starting strain, cultivate it in the seed medium to the logarithmic growth phase, centrifuge and wash to obtain the pure strain, and prepare the sterile sodium alginate (1%, w / v) solution with Modified starch (1%, w / v) is fully mixed in equal volume, and the probiotic concentration after mixing is 10 8 cfu / mL. The mixed liquid is sprayed into CaCl through the spray nozzle 2 (1%, w / v) in solution, Ca 2+ Cross-linking with sodium alginate to form microcapsules. After static solidification for 120 min, physiological saline was washed three times, filtered, and dried to obtain microcapsules.

[0048] Detection Indicator

[0049] a) Appearance characterization: Observed by scanning electron microscope, it is found that the microcapsules are intact, and the bacteria are alive and inside the microcapsules;

[0050] b) Measurement of the embedding rate: The embedding rate is determined according to the method for measuring the embedding rate describe...

Embodiment 3

[0055] Taking CCTCC NO: M 2010163 as the starting strain, it was cultured in the seed medium to the logarithmic growth phase, centrifuged and washed to obtain the pure strain, and the prepared sterile sodium alginate (5%, w / v) solution was combined with Modified starch (5%, w / v) is fully mixed in equal volume, and the probiotic concentration after mixing is 10 10 cfu / mL. The mixed liquid is sprayed into CaCl through the spray nozzle 2 (5%, w / v) in solution, Ca 2+ Cross-linking with sodium alginate to form microcapsules. After static solidification for 120 min, physiological saline was washed three times, filtered, and dried to obtain microcapsules.

[0056] Detection Indicator

[0057] a) Appearance characterization: Observed by scanning electron microscope, it is found that the microcapsules are intact, and the bacteria are alive and inside the microcapsules;

[0058] b) Determination of the embedding rate: The embedding rate is determined according to the method for determining ...

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Abstract

The invention discloses a method for preparing probiotics microcapsule and its application, which belongs to the biological technical field of foodstuffs. According to the invention, the method comprises the following steps: isometrically mixing a sodium alginate solution and a modified starch solution, then mixing with probiotics, spraying into a CaCl2 solution through a nozzle of a sprayer, standing and solidifying, washing by normal saline, filtering, and drying to obtain the microcapsule. The invention has the advantage of substantially raising capability of the probiotics for opposing extremity environment of the human body, and plays health care effect finally, thereby the method provided in the invention provides novel technical ideas for probiotics micro encapsulation.

Description

Technical field [0001] The invention relates to a preparation method and application of probiotic microcapsules, and belongs to the technical field of food biology. Background technique [0002] Probiotics can significantly improve the host's intestinal microecological balance, prevent diarrhea, relieve lactose intolerance, enhance immunity, reduce serum cholesterol, and inhibit the formation of tumor cell precursors. However, since live bacteria preparations enter the digestive tract of animals, it is difficult to withstand the effects of gastric acid, bile salts, digestive enzymes, etc., so it is difficult to ensure a sufficient number of live bacteria to reach the intestine or colonize the intestine to play a role. [0003] After the probiotics prepared by microcapsule technology are used in microecological preparations, they can better maintain the cell activity and greatly improve the targeted colonization of active bacteria when the probiotics are taken orally, and the probio...

Claims

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Application Information

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IPC IPC(8): A23L1/29A23L1/30A23L33/00
Inventor 陈坚刘茜张娟堵国成
Owner JIANGNAN UNIV
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