The invention provides an induction medium for cultivating and breeding Allium fistulosum. The formula of the culture medium comprises KNO3, CaCl2. 2H2O, (NH4) 2SO4, NaH2PO4.H2O, MgSO4. 7H2O, MnSO4. 4H2O, ZnSO4. 7H2O, H3BO3, KI, Na2MoO4. 2H2O, CuSO4. 5H2O, CoCl2. 6H2O, Fe-EDDHA, a corn stigma extracting solution, casein hydrolysate, inositol, sodium pyruvate, fumaric acid, aspartic acid, glycine,sodium humate, riboflavin, vitamin B1, vitamin B6, D-biotin, choline, salicylic acid, sodium butyrate, paclobutrazol, KT, 6-BA, p-hydroxybenzoic acid, sucrose, maltose and phytagel. The invention further provides an induction culture method of the allium fistulosum medicine, the allium fistulosum medicine is inoculated to the induction culture medium for culture after being uniform magnetic fieldtreatment, the callus induction efficiency of the allium fistulosum medicine can be remarkably improved, and establishment of an allium fistulosum medicine cultivating and breeding system is promoted.