The invention discloses a feed for procambarus clarkii and a preparation method and application thereof. The feed comprises grains, cake meal, aquatic plants, snail and clam meat, soybean protein, beer yeast, an attractant, vitamins, table salt, fish meal, rice bran, a shelling element, a binding material, bacillus subtilis, dextrin, zinc sulfate heptahydrate, a licorice root extract and small peptides. The feed is not liable to mildew and deteriorate, free from caking and peculiar smell, good in stability in water, and free from collapse within 3-5h. After the feed for the procambarus clarkiiis used, the ecdysis rate of the procambarus clarkii is increased by 35-45%, the survival rate is increased by 15-25%, the body length is increased by 15-35%, the procambarus clarkii is high in growth speed, the propagation capacity is improved, and the feed is good in palatability, easy to industrialize and broad in application prospects.