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Use of a bacillus pumilus as a growth regulator for lepidopteran insects

A technology of Bacillus pumilus and growth regulator, applied in the field of microorganisms, can solve the problem of no Bacillus pumilus, etc., and achieve the effect of broad application prospects and market value

Active Publication Date: 2020-06-12
HUAIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no technical revelation of Bacillus pumilus as a growth regulator of Lepidoptera insects.

Method used

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  • Use of a bacillus pumilus as a growth regulator for lepidopteran insects
  • Use of a bacillus pumilus as a growth regulator for lepidopteran insects

Examples

Experimental program
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Effect test

Embodiment 1

[0036] 1. Bacillus pumilus strain QHS-X8 is an endophyte isolated and screened from Artemisia annua plants on the campus of Huaihua College in Huaihua City, Hunan Province and in the suburbs. The isolation and screening process is as follows:

[0037] ① Collect samples of Artemisia annua plants: collect Artemisia annua plants, take small pieces of roots, stems and leaves, about 0.15cm×0.15cm in size, rinse with 75% alcohol (20-30s) → rinse with sterile water → 0.2% mercury chloride Rinse (10-20s) → rinse with sterile water several times or directly take the inner layer without contamination;

[0038] ② Coarse sieving of beef extract peptone solid plates: place them on PDA, beef extract peptone, and Gaoshi No. 1 lipid medium plates, culture at 25-30°C in the dark, and culture for 5-20 days. Colonies grow on the plates respectively. Separation methods such as streaking, dilution, and direct use of sterile small scissors to take the tip hyphae, etc., and according to the color re...

Embodiment 2

[0047] The activated Bacillus pumilus strain was inoculated into LB medium, and cultured at 160 rpm for 48 hours at 37°C to obtain a fermentation broth; 10 After soaking in cfu / mL fermentation broth for 3s, the 2nd instar silkworms were fed, and then cultured at 25°C for 21 days.

Embodiment 3

[0049] The activated Bacillus pumilus strain was inoculated into LB medium, and cultured at 160 rpm for 48 hours at 37°C to obtain a fermentation broth; 9 After soaking in cfu / mL fermentation broth for 3s, the 2nd instar silkworms were fed, and then cultured at 25°C for 21 days.

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Abstract

The invention provides a use of Bacillus pumilus as a growth regulator of Lepidoptera insects. The Bacillus pumilus was preserved in the China Center for Type Culture Collection (CCTCC) on August 12, 2010, and the preservation number is CCTCC M2010202. The invention opens up a new field of bacillus pumilus as a high-quality microbial resource, provides beneficial ideas for the cultivation and management of lepidopteran insects, and provides strong support for the prevention and control of agricultural and forestry crops. The growth regulator is green, safe and pollution-free, and has broad application prospects and market value in the agricultural field.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to the use of bacillus pumilus as a growth regulator for lepidopteran insects. Background technique [0002] Bacillus pumilus belongs to the order Bacillus, the family Bacillus, and the genus Bacillus pumilus. It is a non-pathogenic bacterium that widely exists in nature. It has the characteristics of fast reproduction, strong metabolic ability, and rich physiological characteristics. As a dominant population in the soil environment and plant microecology, Bacillus pumilus can produce abundant antibacterial substances to prevent plant diseases, and is an important member of plant disease biocontrol microorganisms. At the same time, Bacillus pumilus has a strong ability to decompose organic matter, and can release decomposing enzymes while multiplying to synthesize a variety of organic acids, enzymes and physiologically active substances to promote crop growth. [0003] Lepidoptera i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033A01K67/04A01G13/00A01N63/22A01P15/00C12N1/20C12R1/07
CPCA01K67/033A01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 李昌灵巫旺胜曾丽芳尹淑婷孙凌云罗灿黄汉青
Owner HUAIHUA UNIV
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