Preparation method of Pueraria lobata polysaccharide and application of Pueraria lobata polysaccharide as a growth promoter

The technology of pueraria polysaccharide and ultrasound is applied in the application of pueraria polysaccharide as a growth promoting agent, and the preparation field of pueraria polysaccharide can solve the problems of complicated preparation steps, harm to human body and environment, low yield and the like.

Inactive Publication Date: 2019-12-10
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The traditional preparation method of kudzu root polysaccharide is: use the organic solvent petroleum ether to repeatedly distill and reflux, precipitate with absolute ethanol, and wash with acetone. It is nece...

Method used

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  • Preparation method of Pueraria lobata polysaccharide and application of Pueraria lobata polysaccharide as a growth promoter
  • Preparation method of Pueraria lobata polysaccharide and application of Pueraria lobata polysaccharide as a growth promoter
  • Preparation method of Pueraria lobata polysaccharide and application of Pueraria lobata polysaccharide as a growth promoter

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preparation example Construction

[0029] 本发明提供了一种葛根多糖的制备方法,包括以下步骤:

[0030] A)将葛根粉与水按照1g:2~20mL混合,在40~80℃下进行超声提取;

[0031] B)将所述超声提取后的料液进行离心,离心后的沉渣与水按照1g:2~20mL混合,在40~80℃下进行超声提取,然后,进行离心;

[0032] C)将步骤B)中两次离心后的上清液混合,进行减压浓缩,得到浓缩液;

[0033] D)将所述浓缩液与乙醇按照体积比为1:2~5混合,进行醇沉;

[0034] E)将所述醇沉后的产物溶液进行离心,得到的上清液经减压浓缩,得到的浓缩液即为葛根多糖。

[0035] 本发明先将葛根粉与水按照1g:2~20mL混合,在40~80℃下进行超声提取。

[0036] 本发明中,所述葛根粉与水的用量比为1g:2~20mL。在本发明的某些实施例中,所述葛根粉与水的用量比为1g:5mL、1g:10mL、1g:15mL或1g:20mL。

[0037] 本发明对所述葛根粉的来源并无特殊的限制,可以为一般市售。

[0038] 葛根粉与水混合后,将混合后的混合液进行超声提取。所述超声提取的温度为40~80℃。在本发明的某些实施例中,所述超声提取的温度为40℃、60℃或80℃。在本发明的某些实施例中,所述超声提取的超声功率为400~800W。在某些实施例中,所述超声提取的超声功率为400W。在本发明的某些实施例中,所述超声提取的时间为0.5~1.5h。在本发明的某些实施例中,所述超声提取的时间为0.5h、1h或1.5h。超声能促使植物组织破壁或变形,使中药有效成份提取更充分。

[0039] 超声提取完成后,将所述超声提取后的料液进行离心。在本发明的某些实施例中,所述离心的转速为2900~3100r / min。在某些实施例中,所述离心的转速为3000r / min。在本发明的某些实施例中,所述离心的时间为15~18min。在某些实施例中,所述离心的时间为15min。

[0040] 离心后,将离心后的沉渣与水按照1g:2~20mL混合,在40~80℃下进行超声提取。

[0041] 本发明中,所述离心后的沉渣与水的用量比为1g:2~20mL。在本发明的某些实施例中,所述离心后的沉渣与水的用量比为1g:5mL、1g:10mL...

Embodiment 1

[0058] 1. Weigh 2g of kudzu root powder into a 50mL conical flask, add 10mL of distilled water to it, and after ultrasonic extraction at 40°C for 0.5h, centrifuge at a speed of 3000r / min for 15min, and mix the centrifuged sediment and water according to 1g: Mix 5mL, ultrasonically extract at 40°C for 0.5h; the ultrasonic power of the two ultrasonic extractions is 400W;

[0059] 2. Mix the supernatant after two times of centrifugation in step 1, and rotary steam at 60°C for 25 minutes to obtain a concentrated solution;

[0060] 3. Mix the concentrated solution obtained in step 2 with ethanol (95% by volume) at a volume ratio of 1:4, and carry out alcohol precipitation; the alcohol precipitation time is 12 hours;

[0061] 4. After the alcohol precipitation is completed, the product solution after the alcohol precipitation is centrifuged at a speed of 3000r / min for 12min, and the obtained supernatant is concentrated under reduced pressure at 60°C for 18min, and the obtained conce...

Embodiment 2

[0063] 1. Weigh 2g of pueraria root powder into a 50mL conical flask, add 20mL of distilled water to it, and after ultrasonic extraction at 40°C for 0.5h, centrifuge at a speed of 3000r / min for 15min, and mix the centrifuged sediment and water with 1g: Mix 10mL, ultrasonically extract at 40°C for 0.5h; the ultrasonic power for both ultrasonic extractions is 400W;

[0064] 2. Mix the supernatant after two times of centrifugation in step 1, and rotary steam at 60°C for 25 minutes to obtain a concentrated solution;

[0065] 3. Mix the concentrated solution obtained in step 2 with ethanol (95% by volume) at a volume ratio of 1:4, and carry out alcohol precipitation; the alcohol precipitation time is 12 hours;

[0066] 4. After the alcohol precipitation is completed, the product solution after the alcohol precipitation is centrifuged at a speed of 3000r / min for 12min, and the obtained supernatant is concentrated under reduced pressure at 60°C for 18min, and the obtained concentrate...

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Abstract

The invention relates to the extraction field of natural active substances, in particular to a preparation method of Pueraria lobata polysaccharide and the application of Pueraria lobata polysaccharide as a growth promoter. The preparation method of the pueraria root polysaccharide comprises the following steps of: A) mixing Pueraria lobata root powder and water according to 1g: 2-20mL, and performing ultrasonic extraction at 40-80 DEG C; B) centrifuging that material liquid after ultrasonic extraction, mixing the centrifuged sediment and water according to 1g: 2-20mL, ultrasonic extraction at40-80 DEG C, and then centrifuging; C) mixing that supernatant after two centrifugation in the step B), and concentrating under reduced pressure to obtain concentrated solution; D) mixing that concentrated solution and ethanol according to the volume ratio of 1: 2-5 to carry out alcohol precipitation; E) centrifuging the product solution after alcohol precipitation, and concentrating the obtainedsupernatant under reduced pressure to obtain the concentrated solution to be Pueraria lobata polysaccharide. The preparation method is simple in operation and has high yield of Pueraria lobata polysaccharide.

Description

technical field [0001] 本发明涉及天然活性物质的提取领域,尤其涉及一种葛根多糖的制备方法及葛根多糖作为促生长剂的应用。 Background technique [0002] 我国具有丰富的中药材资源,近年来,对于中药的具有抗衰老医药价值的天然化合物的探索引起了人们的极大关注。葛根,为豆科植物野葛的干燥根,有退热,透疹,生津止渴,升阳止泻之功效。常用于表证发热,项背强痛,麻疹不透,热病口渴等疾病,具有显著的药用保健价值。现代研究表明,由于广泛的药理学特性,天然多糖已成为控制寿命和促进健康老化的有希望的候选来源。 [0003] 传统的葛根多糖的制备方法为:利用有机溶剂石油醚反复蒸馏回流,无水乙醇沉淀,丙酮洗,需要搭建回流装置,制备步骤繁琐,得率较低,石油醚本身有挥发性、刺激性也比较强,对人体、环境有一定的危害。 Contents of the invention [0004] 有鉴于此,本发明要解决的技术问题在于提供一种葛根多糖的制备方法,本发明提供的葛根多糖的制备方法简单,且葛根多糖的得率较高。 [0005] 本发明提供了一种葛根多糖的制备方法,包括以下步骤: [0006] A)将葛根粉与水按照1g:2~20mL混合,在40~80℃下进行超声提取; [0007] B)将所述超声提取后的料液进行离心,离心后的沉渣与水按照1g:2~20mL混合,在40~80℃下进行超声提取,然后,进行离心; [0008] C)将步骤B)中两次离心后的上清液混合,进行减压浓缩,得到浓缩液; [0009] D)将所述浓缩液与乙醇按照体积比为1:2~5混合,进行醇沉; [0010] E)将所述醇沉后的产物溶液进行离心,得到的上清液经减压浓缩,得到的浓缩液即为葛根多糖。 [0011] 优选的,步骤A)和步骤B)中,所述超声提取的时间为0.5~1h。 [0012] 优选的,步骤B)中,所述离心的转速为2900~3100r / min,所述离心的时间为15~18min。 [0013] 优选的,步骤C)中,所述减压浓缩的温度为60~80℃。 [0014] 优选的,步骤D)中,所述乙醇的体积分数为95%。 [0015] 优选的,步骤E)中,所述醇沉的时间为12~15h。 [0...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P43/00A61P39/00
CPCA61K31/715A61P39/00A61P43/00C08B37/0003
Inventor 童中华邵新月
Owner UNIV OF SCI & TECH OF CHINA
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