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A method for identifying pig body length and/or body height and its special primer pair

A body length and body height technology, applied in the direction of DNA / RNA fragments, recombinant DNA technology, etc., can solve the problems of lengthening generation intervals, increasing breeding costs, and slow genetic progress, so as to reduce breeding costs, increase body size, and operate simple effect

Inactive Publication Date: 2017-01-11
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the body length and body height can only be measured after the animal grows up, on the one hand, the cost of breeding is increased, and on the other hand, the generation interval is lengthened, and the genetic progress is slow.

Method used

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  • A method for identifying pig body length and/or body height and its special primer pair
  • A method for identifying pig body length and/or body height and its special primer pair
  • A method for identifying pig body length and/or body height and its special primer pair

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Embodiment 1

[0029] Embodiment 1, the method for identifying pig body length and / or body height

[0030] 1. Determination of g.14694A>G polymorphism site

[0031] (1) Two Damin hybrid pigs were used as experimental materials, and the genomic DNA of the ear margin tissues were extracted respectively.

[0032] (2) Design and synthesis of primers

[0033] According to the genome DNA sequence of porcine PPARD gene (GenBank Accession Number is GU565976.1), the following primers were designed and synthesized:

[0034] U (upstream primer): 5'-CAGACACCACACCAGAACTACC-3' (SEQ ID No.1);

[0035] D (downstream primer): 5'-CGTGGCTGATGTGTTAGAGGAA-3' (SEQ ID No. 2).

[0036] (3) PCR amplification

[0037] The genomic DNA of Damin hybrid pig obtained in step (1) was used as a template, and U and D were used as primers to carry out PCR amplification to obtain PCR amplification products, which were named product 1 and product 2, respectively.

[0038] PCR amplification system: Genomic DNA 200ng, 10×PCR...

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Abstract

The invention discloses a method or identifying the length and / or height of a pig body and a special primer pair for the method. The invention discloses a primer pair for amplifying a DNA fragment containing g.14694A>G polymorphic site; the g.14694A>G polymorphic site is the 14694-th from the 5' terminal of a sequence of which the GenBank accession number is GU565976.1 and the updated date of the sequence of which the GenBank accession number is GU565976.1 is April 26, 2010. The method disclosed by the invention can be used in pig breeding and can be used for screening the to-be-selected pigs at early stage, the problem that the selection of the well-bred pig takes a long time in the actual production is effectively alleviated, the breeding cost is reduced and the size of the pig body in the actual production can be effectively reduced or improved. The method disclosed by the invention has the advantages of simple process, low cost and high accuracy; by virtue of the method, the automatic direct detection can be achieved; and the method plays a significant role in breeding pigs.

Description

technical field [0001] The invention relates to a method for identifying pig body length and / or body height and a pair of special primers, belonging to the field of biotechnology. Background technique [0002] Pig body size has always been an important part of pig breeding goals. Body size is not only closely related to growth rate and meat production, but also has a greater impact on mating, so its breeding value is very high. However, since the body length and body height can only be measured after the animal grows up, on the one hand, the breeding cost is increased, and on the other hand, the generation interval is lengthened, and the genetic progress is slow. The rapid development of molecular biotechnology and the construction of pig genetic linkage maps have provided a research basis for breeding around quantitative traits such as body length and body height. On the other hand, since pigs are an important model animal for human research, finding genes that affect pig ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 王立刚王立贤张龙超颜华刘欣
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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