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Polydopamine-based biofunction modification method

A biofunctionalized, polydopamine technology is applied in the field of modification of the surface of biological decontamination materials, and can solve the problems that the polydopamine modified layer cannot withstand ultrasonic cleaning, and it is difficult to form a polydopamine layer.

Inactive Publication Date: 2011-04-06
CO WITH LTD LIABILITY OF MEDICAL SCI +1
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  • Abstract
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Problems solved by technology

In the US patent application 20080149566, a biomimetic modification method using polydopamine on the surface of various materials was proposed. However, the oxidation condition required to form polydopamine in this method is only to use a small amount of oxygen dissolved in the solution. It is difficult to form a firm polydopamine layer on the surface of the material, so the applicant pointed out that the polydopamine modification layer formed on the surface of inorganic or metal materials cannot withstand ultrasonic cleaning

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] A. Soak the clean NiTi alloy (Ni 50%, Ti 50%) sheet in alkaline (pH 8.5) dopamine aqueous solution (concentration 2mg / mL) for 0.1 hour, continue to feed oxygen, the oxygen flow rate is 10SCCM , ultrasonic cleaning and drying after taking out the sample to obtain a single layer of polydopamine;

[0013] B. Repeat the process of film deposition, ultrasonic cleaning and drying in step A for 3 times, and then place the sample in an air atmosphere for thermal oxidation treatment (temperature: 50°C, time 4 hours), to obtain four layers firmly bonded to the surface of the sample polydopamine layer;

[0014] C. Immerse the sample prepared in step B into a thiolated polyethylene glycol (PEG) solution with a concentration of 3 mM to react for 0.1 h, take it out, and then ultrasonically clean and dry it to obtain a surface PEG-functionalized sample.

Embodiment 2

[0016] A. Soak a clean titanium alloy (Al: 6%, V4%, the rest is titanium) sheet in alkaline (pH 7.2) dopamine aqueous solution (concentration 1 mg / mL) for 6 hours, and then continue to pass through Oxygen for 6 hours, the oxygen flow rate is 100SCCM, the sample is taken out and ultrasonically cleaned to obtain a single layer of polydopamine;

[0017] B. Repeat the process of soaking the sample, oxidation and ultrasonic cleaning in step A 4 times, and then place the sample in an air atmosphere for thermal oxidation treatment (temperature: 100°C, time: 10 hours), that is, five layers are firmly bonded to the surface of the sample polydopamine layer;

[0018] C. Immerse the sample immobilized with five layers of polydopamine in a heparin solution with a concentration of 10 mg / mL for 6 hours, take it out, and clean it ultrasonically to obtain a sample with surface heparinization.

Embodiment 3

[0020] A. Soak the clean graphite sheet in alkaline (pH 8) dopamine aqueous solution (concentration is 5mg / mL) for 24 hours, continue to feed oxygen for 24 hours, the oxygen flow rate is 20SCCM, take out the sample and then ultrasonically clean it to obtain Monolayer polydopamine.

[0021] B. Repeat the process of soaking the sample, oxidation and ultrasonic cleaning in step A 9 times, and then place the sample in an air atmosphere for thermal oxidation treatment (temperature: 120°C, time: 24 hours), that is, ten layers are firmly bonded to the surface of the sample polydopamine layer.

[0022] C. Immerse the sample immobilized with ten layers of polydopamine in a glucose oxidase solution with a concentration of 1 mg / mL for 24 hours, take it out, and clean it ultrasonically to obtain a sample immobilized with glucose oxidase.

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Abstract

The invention discloses a polydopamine-based biofunction modification method. The method comprises the following steps of: soaking clean inorganic / metal materials in dopamine alkaline aqueous solution with a pH value of 7.2 to 10, introducing oxygen to fully oxidize the clean inorganic / metal materials to obtain a monolayer polydopamine modification layer, drying, placing the material in the air atmosphere at 50 to 200 DEG C, performing thermal oxidation to obtain a multi-layer firmly-combined high-reactivity polydopamine layer, and soaking the treated material into the amino-containing and sulfydryl-containing biofunctional molecular solution to obtain a firmly combined biofunction simulate modification layer on the surface of the materials. The polydopamine modification layer obtained bythe method has excellent anti-deformation performance, stability and reactivity, can directly react with amino or the sulfydryl in the biomolecule to immobilize the bioactive molecule on the surface by covalent bonds; moreover, the process is simple, the condition is mild, and the method is easy to implement.

Description

technical field [0001] The invention relates to the surface modification technology of inorganic or metal materials, in particular to the surface modification technology of artificial organ materials, biological sensor materials, anti-corrosion materials and biological decontamination materials. Background technique [0002] The biofunctional modification method on the surface of metal or inorganic materials is still mainly a physical coating method at present, and the physical coating between the organic layer and the metal substrate is often easy to fall off due to too weak force, while the chemical modification method, such as silane Chemical methods and monolayer self-assembly methods often require a specific chemical composition of the substrate, which cannot be adapted to implant materials with complex chemical compositions. Marine mussels in nature can firmly fix themselves on rocks (mainly composed of various inorganic minerals) or ships (mainly composed of metal mat...

Claims

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Application Information

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IPC IPC(8): B05D3/10B05D5/00B05D1/18
Inventor 黄楠翁亚军杨志禄宋强罗日方冷永祥杨苹王进陈俊英孙鸿吴熹李全利
Owner CO WITH LTD LIABILITY OF MEDICAL SCI
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