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A kind of concentrated cultivation method of Cyclotella menieli seawater strain

A technique of Cyclotella menielis and its cultivation method, which is applied in the field of concentrated culture of Cyclotella menieli seawater strains, to achieve the effects of promoting cell growth, increasing cell density, and enhancing thickness

Active Publication Date: 2021-07-06
SHENZHEN INST OF GUANGDONG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is no set of standard methods for concentrated cultivation of Cyclotella menieli

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Culture method of concentrated plum algae in indoor cement pool

[0036] Cement pool disinfection: the specification is 1.5m × 30m 2 Indoor cement pool cleaning, brush drying around and pool, splashing with potassium permanganate (0.1 ~ 0.2%) splashing around, 10min, was purified with water.

[0037] Sea water disinfection: The filtration of the sea water is about 1.2m high, and the water is disinfected with 450 g of the commercially available strong chloride. The water is disinfected with 900 g of sodium thiosulfate, and the continuous aeration is 5 to 7 hours, and the lattice can be used.

[0038] Add a nutrition salt: According to the formulation of the medium, urea 2.0 kg, 0.36 kg of hydrogen phosphate, 0.72 kg of sodium gasei, 0.36 kg of ferric chloride, 1.25 kg of sodium carbonate, 0.162 kg of ethylenediamine tetracetate; After the nutrient salt is completely dissolved with disinfection seawater, splaish into the pool, and the strong gas is evenly distributed.

[0039...

Embodiment 2

[0042] Outdoor plastic drum culture concentrated Menny small cyclrate

[0043] Plastic bucket cleaning: plastic bucket is white light-transmitted bell, volume is 2m 3 , Rinse the adhesion of the drum in the inner wall with a high pressure water gun.

[0044] Sea water disinfection: Add filtering sea water approximately 1.8M 3 The water was disinfected with water by 37.5 g of water for 6 to 8 h; then in 45 g of sodium thiosulfate, continuous aeration 5 to 7 hours, the lattice can be used.

[0045] Add a nutrition salt: According to the formulation of the culture medium, weigh the urea 110g, the potassium hydrogen phosphate is 18g, sodium chlorite 36g, iron chloride 8g, sodium carbonate, sodium ethylenediamine, sodium diethyl acetate 8.4g; nutrient salt separately with disinfection After the seawater is completely dissolved, pour into the bucket, and the strong gas is evenly distributed.

[0046] Inoculation, inoculation time is generally 8 ~ 10 in the morning, 500L growing Menny Co...

Embodiment 3

[0049] Cultivation of cultured plum algae

[0050] Cement pool disinfection: the specification is 7.5m × 30m 2 Cement pool cleaning, brush, clean and pool, splashing with potassium permanganate (0.1 ~ 0.2%), was sprinkled around, 10 min, was purified.

[0051] Sea water disinfection: adding the water filter for about 1.2m high, and the water is disinfected with 3.75 kg of water for 6 to 8 hours; then in the use of 4.5kg sodium thiosulfate, continuous aeration 5 ~ 7h, continuously aerated, the lattice can be used. use.

[0052] Add a nutrition salt: According to the culture base formulation, the urea 130mg / L, the potassium hydrogen phosphate is 8mg / L, sodium mese 22mg / L, iron chloride 2mg / L, sodium carbonate 40mg / L, ethylenediamine tetracetate Sodium 4.2mg / L; Nutritat Solidly dissolved with disinfection seawater, splash into the pool, strong charge to make the nutrient salt evenly distributed.

[0053] Inoculation culture: Inoculation time, in the morning 8 ~ 10, the 10...

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Abstract

The invention provides a concentrated cultivation method of Cyclotella menieli seawater strain, which belongs to the technical field of microalgae cultivation. Inoculate the seawater strain of Cyclotella menieli into the seawater culture medium, and culture it under ventilation at 20-35°C and 1500-3000 Lux, and collect the concentrated algae liquid after the algae settle; the seawater culture medium contains the following components per liter of seawater: urea 58 ~65mg, dipotassium hydrogen phosphate 8~12mg, sodium metasilicate 18~22mg, ferric chloride 2~4mg, sodium carbonate 32~40mg and edetate disodium 4.2~4.6mg; N:Si is 14~16:5~7:0.5~1.5, and the pH value is 7.8~9.0. The cultivation method increases the cell density of Cyclotella menieli under the condition of ensuring intact algae cell activity, increases the sedimentation speed by more than 2 times, shortens the sedimentation time, and saves the cost of large-scale cultivation of Cyclotella menieli.

Description

Technical field [0001] The present invention belongs to the technical field of microalgae culture, and in particular to a concentration culture method for seawater of Menechethocyae. Background technique [0002] Microalgae is a very important component of the shrimp farming ecosystem, absorbing solubility nitrogen for growth, improves dissolving oxygen and pH in the water, inhibits eucalyptus growth, and can be used as a hydrostatic animal's biological bait microalgae to maintain breeding The ecological balance of the system, accelerated substance circulation, and purifying a breeding environment are extremely important. By targeting useful microalgae is an important technical measures to optimize microalgae community. [0003] Menny Cycloalkah is a common kind of advantage species in the lard high-level pool in the Western Yuexi, forming a water environment suitable for shrimp growth, commonly known as "diatom" tea brown, tea brown water environment in shrimp physiological resp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 李长玲黄翔鹄王培明
Owner SHENZHEN INST OF GUANGDONG OCEAN UNIV
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