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Blood serum or blood plasma diluting solution applied to detection method of trace gelatin particle agglutinated HIV-1 (Human Immunodeficiency Virus-1) recent infection

A technology for HIV-1 and infection detection, applied in the biological field, can solve the problems of high price, limited wide application, and inability to provide alone

Inactive Publication Date: 2012-07-25
李洪
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since the diluent of the serum or plasma sample used in this method is the Dilsim of the Vironostika HIV antibody special detection reagent produced by BioMerieux (Raleigh, NC) TM ll diluent, so the price is expensive, and the effective shelf life is very short. Generally, it cannot be provided separately, and it cannot be purchased in China
Therefore, the wide application of this method in our country is greatly limited.

Method used

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  • Blood serum or blood plasma diluting solution applied to detection method of trace gelatin particle agglutinated HIV-1 (Human Immunodeficiency Virus-1) recent infection
  • Blood serum or blood plasma diluting solution applied to detection method of trace gelatin particle agglutinated HIV-1 (Human Immunodeficiency Virus-1) recent infection

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Experimental program
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Embodiment 1

[0014] Embodiment 1: a kind of serum or blood plasma sample diluent that is applied to the detection method that trace gelatin particle agglutination HIV-1 recently infects, and this diluent is made up of 1.27 gram Na 2 HPO 4 , 0.3 g NaH 2 PO 4 10 milliliters of 30% Triton X 100, 200 milligrams of preservative NaN3, and 10 grams of antibody protective agent bovine serum albumin were added to the buffer prepared by dissolving 0.85 gram of NaCl in 1000 milliliters of deionized water, Mix thoroughly to obtain a homogeneity.

[0015] In order to determine the diluent of the present invention and Dilsim TM ll Consistency of dilutions, using 450 confirmed HIV-positive serum specimens of unknown infection time, for recent infection detection assays. Each specimen is used the diluent of the present invention and Dilsim according to 6 gradients of 1:10, 1:100, 1:1000, 1:10000, 1:20000, 1:40000 TM ll diluents were diluted, for 3 consecutive days, once a day by two different peopl...

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Abstract

The invention provides a blood serum or blood plasma sample diluting solution applied to a method for detecting trace gelatin particle agglutinated HIV-1 (Human Immunodeficiency Virus-1) recent infection, belonging to the field of a biological technology. The invention particularly provides a reagent applied to detecting the HIV-1 recent infection. The diluting solution is prepared from a buffering solution, TritonX100 with the concentration of 30%, NaN3 and bovine serum albumin by mixing according to a ratio. The diluting solution disclosed by the invention can completely replace a DilsimTM11 diluting solution and can be used for a detection method of the trace gelatin particle agglutinated HIV-1 recent infection.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a reagent used in detecting HIV-1 recent infection. Background technique [0002] AIDS is an infectious disease, and testing for HIV (HIV) is an important way to find out who is infected. In order to grasp its epidemic trend and evaluate the effect of prevention and control, all countries in the world want to use HIV new infection rate to evaluate, and the calculation of new infection rate needs to have recent HIV infection data. [0003] HIV recent infection data can be obtained through a variety of laboratory testing HIV recent infection methods. Laboratory methods for detecting recent HIV infection can be classified according to whether the specimen used is pre-seroconversion or post-seroconversion. The method used for the specimen before seroconversion is to detect the P24 antigen or HIV-1 RNA of HIV-1 in the specimen; the method used for the specimen after seroconversion inclu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
Inventor 李洪
Owner 李洪
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