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Method for fast testing human ABO/Rh/MN blood type and kit

A technology for detecting people and kits, which is applied in the field of rapid detection of human ABO/Rh/MN blood types, can solve the problems of long test time, difficult to judge results, and low sensitivity, and achieve the effect of simple operation and easy operation.

Active Publication Date: 2009-12-16
INTEC PROD INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method requires corresponding matching reagents, and the reagents need to be stored at low temperature, and the results are difficult to judge
[0006] Other blood group detection methods also have their own shortcomings, such as the time-consuming neutralization test method, and the sensitivity is relatively low
The dissociation experiment and the mixed agglutination reaction method have high operating requirements, and each experiment needs to repeatedly explore the conditions, and its sensitivity is not high; the synergistic agglutination reaction method is to sensitize Staphylococcus aureus protein A (SPA) with an IgG antibody, and observe the grape Whether the cocci agglutinated to determine the blood type of the tested sample, but this method has high requirements for reagent preservation; although radioimmunoassay and enzyme-linked immunoassay methods have high sensitivity, they are time-consuming, not suitable for single-person testing, and cannot be used for on-site testing
[0007] In short, the currently commonly used clinical blood typing methods have problems such as long testing time, high manual labor intensity, and the results are greatly affected by subjective bias and the possibility of contamination.

Method used

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  • Method for fast testing human ABO/Rh/MN blood type and kit
  • Method for fast testing human ABO/Rh/MN blood type and kit
  • Method for fast testing human ABO/Rh/MN blood type and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: Preparation and detection steps of the detection reagent strip in the human ABO blood group kit

[0046] (1) Immobilize blood type antibodies (anti-A, anti-B antibodies) on the sample pad:

[0047] Take a high-titer blood type monoclonal anti-A anti-B antibody (produced by Changchun Broad Biotechnology Co., Ltd.), and dilute it to 1 ml with 5 mM phosphate buffer solution, and the final titer is 64. Add antibody immobilization protectant (composed of 5% bovine serum albumin, 5% sucrose, 5% PVP, 5% calf serum phosphate buffer) to the diluted antibody solution to promote the immobilization of the antibody on the glass fiber mat and save. Spread the antibody on the pre-activated glass fiber mat according to the ratio of spreading 5cm×5cm glass fiber mat for every 1.5ml of diluted antibody, then dry it at 37°C and seal it for storage.

[0048] (2) Assembly of rapid detection reagent strips:

[0049] Paste a 20mm wide flushing glass fiber pad in the center of...

Embodiment 2

[0052] Embodiment 2: Preparation and detection steps of the detection reagent strip in the human Rh blood type detection kit

[0053] (1) Immobilize blood group antibody (anti-D antibody) on the sample pad:

[0054] Take a high-titer blood type monoclonal anti-D antibody (produced by MILLIPORE, USA) and dilute it to 1 ml with 5 mM phosphate buffer solution, and the final titer is 32. Add antibody immobilization protection agent (composed of the PBS solution of 5% albumin, 5% sucrose, 5% PVP, 5% calf serum) in the diluted antibody solution so as to promote the fixation and preservation of the antibody on the glass fiber mat, and A reaction enhancer (phosphate buffer containing 2% Tween20 and 2% Triton X-100) was added to promote the subsequent immune response. Spread the antibody on the pre-activated glass fiber mat according to the ratio of spreading 5cm×5cm fiber mat per 1.5ml of diluted antibody, then dry it at 37°C, and then seal it for storage (see image 3 ).

[0055] ...

Embodiment 3

[0056] Embodiment 3: Preparation and detection steps of the detection reagent strip in the human MN blood type detection kit

[0057] The preparation and detection steps of the rapid detection reagent strip of the human MN blood group detection kit are similar to the preparation and detection steps of the rapid detection reagent strip of the human Rh blood type detection kit (see Figure 4 ).

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Abstract

The invention relates to a method for fast testing human ABO / Rh / MN blood type and a kit used in the method. The method and the testing kit follow the principle of immunochromatography and realize the blood type test by observing whether residual red substances resulted from the agglutination of erythrocytes exist or not after the antigen antibody reaction of blood type substances. The method has simple operation and can avoid various defects in present ordinary blood type testing methods. In the method, the loading amount of tested blood sample is no more than 10 microliter, and the whole test process lasts no more than 2 minutes.

Description

field of invention [0001] The invention relates to a method for rapidly detecting human ABO / Rh / MN blood type and a corresponding kit. Background of the invention [0002] The blood typing method is a routine method for dividing human red blood cells into four different types of A, B, AB, and O, as well as the Rh blood group system and the MN blood group system. The general method of identifying blood type is to mix the tested red blood cells with a reagent containing blood type antibodies, and after the two react, the blood type of the tested red blood cells can be judged by observing whether the red blood cells undergo cell agglutination. In such methods, red blood cells are first isolated from the patient's serum. Mix an appropriate amount of red blood cells with the test solution (containing anti-A serum, anti-B serum or anti-D serum or anti-M serum, anti-N serum) introduced into the test tube or on the glass slide, and other reagents that facilitate the immune reaction ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/80
CPCG01N33/80
Inventor 汪大明杨文肖江群王保丹
Owner INTEC PROD INC
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