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Method for producing animal feed from oil-tea-cake dregs fermented by neurospora crassa

A technology of Neurospora robusta and Camellia oleifera cake, applied in animal feed, animal feed, additional food elements, etc., can solve the problems of unfavorable digestion and absorption of nutrients, improve the quality of tea meal, increase utilization and digestibility , reduce the effect of crude fiber

Active Publication Date: 2009-11-18
浙江播恩生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Someone utilizes Aspergillus niger, Aspergillus oryzae, Trichoderma viride and Candida utilis to make feed through compound synergistic fermentation of camellia oleifera cake, and its crude fiber degradation rate is only about 10%, and the crude protein improvement rate is about 30%, which is still low Facilitate the animal body to digest and absorb nutrients well

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The oil-tea camellia cake after extracting the tea saponin is pulverized and passed through a 40-100-mesh sieve. Weigh the cake 7.5g, bean dregs 1.5g, (NH 4 ) 2 SO 4 1.0 g is used as a production fermentation medium, and the ratio of material to liquid is adjusted to 1:1 with tap water of pH 7.0, sterilized by high-temperature steam for 3 minutes to 10 minutes, and then cooled to be inoculated. At the same time, the fermented cells were activated in PDA liquid medium, enriched and cultured for 42h-60h. Afterwards, the enriched Neurospora brassica was inserted into the actual production solid-state fermentation medium to ferment for 98 hours according to the inoculum amount of 8%, and the temperature was adjusted to 25°C-45°C. After the fermentation is finished and dried, the tea dregs fermented feed is obtained. It was determined that the increase rate of crude protein of tea meal fermented by Neurospora crassa was 15%-40%, the degradation rate of crude fiber was 3...

Embodiment 2

[0015] The oil-tea camellia cake after extracting the tea saponin is pulverized and passed through a 40-100-mesh sieve. Take by weighing this cake 65g, bean dregs 27g, (NH 4 ) 2 SO 4 8g is used as the production fermentation medium, and the tap water with pH 7.0 is used to adjust the ratio of material to liquid to 1:1, after being sterilized by high-temperature steam for 3 minutes to 10 minutes, it is cooled to be inoculated. . At the same time, the fermented cells were activated in PDA liquid medium, enriched and cultivated for 48h-72h. Afterwards, the enriched Neurospora brassica was inserted into the actual production solid-state fermentation medium to ferment for 48 hours according to the inoculum amount of 10%, and the temperature was adjusted to 20°C-45°C. After the fermentation is finished and dried, the tea dregs fermented feed is obtained. The increase rate of crude protein of tea meal fermented by Neurospora crassa was determined to be 15%-40%, the degradation ...

Embodiment 3

[0017] The camellia oleifera cake is dried and pulverized, and passed through a 40-100-mesh sieve to extract tea saponin. Take residue 7.0Kg after extraction, bean dregs 1.9Kg, (NH 4 ) 2 SO 4 1.1Kg is used as the production fermentation medium, and the tap water with pH 7.0 is used to adjust the ratio of material to liquid to 1:1. After being sterilized by high-temperature steam for 3 minutes to 10 minutes, it is cooled to be inoculated. At the same time, the fermented cells were activated in PDA liquid medium, enriched and cultivated for 48h-60h. Afterwards, the enriched Neurospora brassica was inserted into the actual production solid-state fermentation medium to ferment for 60 hours according to the inoculum amount of 5% to 10%, and the temperature was adjusted to 20°C to 50°C. After the fermentation is finished and dried, the tea dregs fermented feed is obtained. It was determined that the increase rate of crude protein of tea meal fermented by Neurospora crassa was 1...

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Abstract

The invention provides a method for producing animal feed from oil-tea-cake dregs fermented by neurospora crassa, which is characterized in that the preparation method is used for producing novel animal feed by the fermentation of neurospora crassa. The method comprises the following specific steps of: crushing and screening the tea-cake dregs with tea saponin extracted by oil; mixing the tea-cake dregs with soybean dregs or rice bran and little inorganic salt so as to be taken as a fermentation medium for production; adjusting the material-liquid ratio by water with the pH value of 7.0; subsequently carrying out heating and sterilizing treatment; subsequently carrying out cooling treatment, subsequently inoculating the activated and enriched zymocyte stains on the solid fermentation medium for production; and controlling various fermentation conditions. The method has the advantages of: 1. greatly reducing crude fiber, increasing the content of Beta-carotenes, improving the quality of tea dregs and increasing the utilization ratio and digestibility of the feed; 2. small investment for raw material and production equipment and large benefits by fermenting the tea dreg feed by microorganism; and 3. changing the waste into treasure and reducing the environmental pollution by utilizing the microorganism to ferment the tea dregs.

Description

technical field [0001] The invention relates to a method of using tea meal as main material and bean dregs as auxiliary material to be fermented by Neurospora fortae, in particular to a method for producing animal feed by fermenting tea-oleifera cake by Neurospora fortae. Background technique [0002] Camellia oleifera in my country has a large cultivation area and abundant resources, and its output ranks first in the world. After the oil is pressed every year, there is a large amount of cake residue, which is equivalent to three times that of tea oil. However, due to the bitter taste and toxicity of camellia oleifera cake, it is basically discarded or directly used as fuel at present, resulting in a great waste of resources. [0003] According to research, tea meal is rich in nutrition, and the content of total sugar, crude protein and nitrogen-free extract is very high, especially the total sugar and nitrogen-free extract, the content is more than 50%, which is a good raw...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/14A23K1/16A23K10/12A23K10/37A23K20/20
CPCY02P60/87
Inventor 邓泽元贾才华肖玉娟范亚苇李静
Owner 浙江播恩生物技术有限公司
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