Method for preparing protein feedstuff through straw solid-state fermentation by using neurospora crassa
A technology of Neurospora crassa, solid-state fermentation, applied in animal feed, animal feed, application, etc., can solve the problems of limited source of fermentation substrate and high price, improve the quality and palatability of rice straw, reduce feed cost, crude protein high content
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Embodiment 1
[0038] 1. Preparation of solid-state fermentation medium
[0039] First crush the rice straw and pass through a 40-mesh sieve to obtain rice straw powder; then soak the rice straw powder in an acetic acid solution with a pH of 5.0 for acid treatment, take out the rice straw powder after 30 minutes of acid treatment, and evaporate the volatile acid , make the acidified rice straw powder of pH5.0;
[0040] Add 5g of peptone to 1L of tap water, KH 2 PO 4 2g, MgSO 4 0.8g, NH 4 Cl3g, after stirring and dissolving, a liquid medium was prepared;
[0041] Mix the above-mentioned acidified rice straw powder with the above-mentioned liquid medium according to the weight ratio of 70:30 to prepare a solid-state fermentation medium;
[0042] 2. Solid state fermentation
[0043] Inoculate Neurospora crassa in sterilized PDA solid medium for activation, and the activated strains are transferred to the above-mentioned solid fermentation medium for expanded culture;
[0044] Spread the ...
Embodiment 2
[0047] 1. Preparation of solid-state fermentation medium
[0048] First, the rice straw is crushed and passed through an 80-mesh sieve to obtain rice straw powder; then the rice straw powder is soaked in an acetic acid solution with a pH of 4.5 for acid treatment, and after 10 minutes of acid treatment, the rice straw powder is taken out and the volatile acid is evaporated. , the acidified rice straw powder of pH4.5 is obtained;
[0049] Add 3g of peptone to 1L of tap water, KH 2 PO 4 3g, MgSO 4 0.5g, NH 4 Cl1g, after stirring and dissolving, a liquid medium was prepared;
[0050] Mix the above-mentioned acidified rice straw powder with the above-mentioned liquid medium according to the weight ratio of 80:20 to prepare a solid-state fermentation medium;
[0051] 2. Solid state fermentation
[0052] Inoculate Neurospora crassa in sterilized PDA solid medium for activation, and the activated strains are transferred to the above-mentioned solid fermentation medium for expan...
Embodiment 3
[0055] 1. Preparation of solid-state fermentation medium
[0056] First crush the rice straw and pass through a 60-mesh sieve to obtain rice straw powder; then soak the rice straw powder in an acetic acid solution with a pH of 5.5 for acid treatment, take out the rice straw powder after 60 minutes of acid treatment, and evaporate the volatile acid , make the acidified rice straw powder of pH5.5;
[0057] Add 8g of peptone to 1L of tap water, KH 2 PO 4 1g, MgSO 4 1.5g, NH 4 Cl4g, after stirring and dissolving, a liquid medium is obtained;
[0058] Mix the above acidified rice straw powder with the above liquid medium according to the weight ratio of 60:40 to prepare a solid fermentation medium;
[0059] 2. Solid state fermentation
[0060] Inoculate Neurospora crassa in sterilized PDA solid medium for activation, and the activated strains are transferred to the above-mentioned solid fermentation medium for expanded culture;
[0061] Spread the solid-state fermentation me...
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