Method for preparing biochrome by double-bacteria fermentation using waste water rich in amino acid as base material

A technology of biological pigments and amino acids, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of high fermentation cost and low yield, and achieve the effects of high expression intensity, high yield, and increased yield

Inactive Publication Date: 2020-07-14
优贝佳(青岛)生物科技有限公司
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This process still has the problems of high fermentation cost and low yield.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing biochrome by double-bacteria fermentation using waste water rich in amino acid as base material
  • Method for preparing biochrome by double-bacteria fermentation using waste water rich in amino acid as base material
  • Method for preparing biochrome by double-bacteria fermentation using waste water rich in amino acid as base material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Preparation of seed medium

[0033] Neurospora crassa (Neurospora crassa ATCC10815) and black fungus (Auricularia auricula) were inoculated on PDA plates from the slant preservation tubes and cultured at 28°C for 10 days, and the colony morphology was shown in the figure ( figure 1 ). During the 10-day cultivation process, the seed medium was prepared;

[0034] Seed medium: 200 ml of milk factory waste water (after filtering to remove impurities, the total content of main components amino acid and protein 0.6%, oil 0.02%), add glucose 6g, peptone 4g, CaCl 2 0.07g, vitamin B 0.1g, vitamin C 0.1g, adjust pH 7.0-8.0. The culture medium was sterilized at 115°C for 30 min under high temperature and high pressure.

[0035] (2) Preparation of primary seed solution

[0036] On the PDA plate, 8 pieces of 0.5mm × 0.5mm bacterium blocks were picked, respectively inoculated in the seed culture medium of 200mL, and 26 ℃, 150rpm shake flask cultured for 3 days, as the first-...

Embodiment 2

[0046] (1) Preparation of seed medium

[0047] Neurospora crassa (Neurospora crassa ATCC10815) and black fungus (Auricularia auricula) were inoculated on PDA plates from slant preservation tubes and cultured at 28°C for 10 days. During the 10-day cultivation process, the seed medium was prepared;

[0048] Seed medium: 2000 ml of waste water rich in amino acids, milk factory waste water (after filtering to remove impurities, the total content of amino acids and protein in the main components is 0.6%, and oil 0.02%), add glucose 60g, peptone 30g, CaCl 2 0.6g, vitamin B 0.5g, vitamin C 0.5g, adjust the pH to 7.0-8.0. The medium was sterilized at 115°C for 30 min under high temperature and high pressure.

[0049] (2) Preparation of primary seed solution

[0050] Pick 20 pieces of 0.5mm×0.5mm bacterial pieces on the PDA plate, inoculate them in 2000mL seed culture medium respectively, and cultivate them at 26°C and 150rpm for 4 days as the primary seed solution.

[0051] (3) P...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for preparing biological pigments by dual-bacteria fermentation using waste water rich in amino acids as a base material. The method uses Neurospora crassa and Auricularia auricula as fermentation strains, prepares a fermentation medium with waste water rich in amino acids, vibrates and ferments at 25-32°C, and extracts and concentrates the obtained fermentation liquid to obtain Biochrome. The invention utilizes amino acid-rich waste water to prepare a fermentation medium, and then utilizes dual-species coupled fermentation to produce pigments, effectively combines the treatment of amino acid-rich waste water and waste water resource utilization, and reduces the cost of producing biological pigments. The bio-pigment produced has the characteristics of high yield, environmental protection, strong ecological acceptability, etc., and has a good dyeing effect on protein.

Description

technical field [0001] The invention relates to a method for preparing biological pigments by double-bacteria fermentation using waste water rich in amino acids as a base material, and belongs to the field of microbial fermentation. Background technique [0002] It is the need of current development to greatly improve the large-scale production and application level of food and feed additives such as bio-pigments and bio-flavors. In recent years, countries around the world have successively formulated regulations to eliminate most toxic chemically synthesized pigments. Those natural pigments that have coloring functions, nutrition and health effects, and can endow food with new functions will have broad market prospects. The annual trade volume of natural pigments in the world is 1.3 billion US dollars, the annual demand is about 15,000 tons, and it is increasing at an annual rate of 11%. At present, most varieties of natural pigments come from plant extraction, but their d...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P1/02G01N33/68C12R1/645
CPCC12N1/14C12P1/02C12N1/145C12R2001/645G01N33/68
Inventor 夏文杰王强宋凯凯
Owner 优贝佳(青岛)生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap