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Method for extracting natural carotenoid from neurospora crassa and spores thereof

A technology of Neurospora brassica and carotene, applied in natural dyes, chemical instruments and methods, azo dyes, etc., can solve the problems of carotenoid loss, conidia wall thickness, etc., achieve short wall breaking time, Effects in a short time and mild conditions

Inactive Publication Date: 2012-02-15
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the pigments produced by the bacteria are intracellular pigments, and the conidia have thick and hard walls. It is difficult to extract the carotenoids directly by organic solvent extraction, and the carotenoids are sensitive to factors such as light, temperature and oxygen. The complicated extraction process is easy to cause the loss of carotenoids in the extraction process, so a simple, efficient and fast extraction process is very important for the extraction of carotenoids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The solid-state culture of Neurospora stouti was dried at 45-60°C and then pulverized. The coarse powder was passed through a 40-mesh sieve. The coarse powder of the culture was broken by a 0.8mol / L hydrochloric acid solution at room temperature for 1-5min. The acid solution was added in an amount of 0.5~2L / kg culture, washed with water after wall breaking, squeezed filtration or suction filtration under reduced pressure, repeated washing and filtration twice until neutral, the obtained filter residue was leached with acetone for 5~10min, and the material-liquid ratio was 5~ The 10L / kg filter residue is leached twice, the extracts are combined, and the extracts are evaporated under reduced pressure at 45-50°C to recover the solvent to obtain the crude carotenoid extract. The extraction rate of pigment can reach more than 98%.

Embodiment 2

[0017] The spores of Neurospora stout were dried at 45-60°C and then broken by a 0.8mol / L hydrochloric acid solution at room temperature for 1-3min. The acid solution was added in an amount of 1.0-3.0L / kg of the spores. After the wall was broken, washed with water and squeezed Filtration or suction filtration under reduced pressure, repeat the water washing and filtration operations twice until neutral, the obtained filter residue is leached with acetone for 5-8min, the material-to-liquid ratio is 35-50L / kg filter residue, leached twice, combined the extract, the extract The carotenoid crude extract is obtained after the solvent is recovered by evaporation under reduced pressure at 45-50°C. The extraction rate of pigment can reach more than 96%.

Embodiment 3

[0019] After the liquid culture of Neurospora stouti was washed with water and filtered, the obtained mycelium was broken by a 1.0mol / L hydrochloric acid solution at room temperature for 5-10min, and the acid solution was added in an amount of 1.0-2.0L / kg of mycelium, and the wall was broken. After finishing, washing with water, extrusion filtration or vacuum suction filtration, repeating the washing and filtration operations twice until neutral, the obtained filter residue is leached with ethyl acetate for 8-10min, the material-to-liquid ratio is 15-25L / kg filter residue, leaching two Second, the extracts were combined, and the extracts were evaporated under reduced pressure at 45-50°C to recover the solvent to obtain the crude carotenoid extract. The extraction rate of pigment can reach more than 95%.

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Abstract

The invention relates to a method for extracting natural carotenoid from neurospora crassa and spores thereof, which comprises the following steps of: drying the neurospora crassa and spores thereof at 30-60 DEG C, pulverizing the culture into coarse powder, breaking walls for 30 s to 30 min by using a 0.1-3 mol / L hydrochloric acid solution through the normal temperature acid method, filtering the solution, leaching the filter residue for 1-60 min by using an organic solvent after water washing, evaporating the extracting solution at reduced pressure, and recovering the solvent to obtain coarse extract. Compared with the traditional carotenoid extraction method, the method provided by the invention has the advantages of simple process, high pigment extraction rate, low production cost, high carotenoid purity and the like.

Description

[0001] technical field [0002] The present invention is a kind of from Neurospora sturdy ( Neurospora.crassa ) and a method for extracting natural carotenoids from spores thereof, belonging to the field of production and processing of natural pigment additives. Background technique [0003] Natural carotenoids are mainly used as natural pigments and nutritional fortifiers, which can prevent human vitamin A deficiency and prevent eye diseases. In recent years, studies have shown that carotenoids play a significant role in preventing cancer, preventing cardiovascular disease and delaying aging. They have been widely used in food and feed additives, medicine and health care and cosmetics industries. At the same time, natural pigments have synthetic pigments. The incomparable physiological function and safety are very suitable for the current trend of green and environmental protection. It is a popular product in the international market and has a very broad market prospect. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00
Inventor 邓泽元刘志刚李静
Owner NANCHANG UNIV
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