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Method for preparing protein feed by solid-state fermentation of rice straw with Neurospora crassa

A technology of Neurospora crassa and solid-state fermentation, applied in animal feed, animal feed, application, etc., can solve the problems of limited source of fermentation substrate and high price, improve rice straw quality and palatability, reduce feed cost, and have good activity Effect

Active Publication Date: 2014-10-01
SHAANXI SHIYANG AGRI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the sources of fermentation substrates such as tea cake cakes and brewer's grains are limited and relatively expensive

Method used

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  • Method for preparing protein feed by solid-state fermentation of rice straw with Neurospora crassa

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Preparation of solid-state fermentation medium

[0039] First crush the rice straw and pass through a 40-mesh sieve to obtain rice straw powder; then soak the rice straw powder in an acetic acid solution with a pH of 5.0 for acid treatment, take out the rice straw powder after 30 minutes of acid treatment, and evaporate the volatile acid , make the acidified rice straw powder of pH5.0;

[0040] Add 5g of peptone to 1L of tap water, KH 2 PO 4 2g, MgSO 4 0.8g, NH 4 Cl3g, after stirring and dissolving, a liquid medium was prepared;

[0041] Mix the above-mentioned acidified rice straw powder with the above-mentioned liquid medium according to the weight ratio of 70:30 to prepare a solid-state fermentation medium;

[0042] 2. Solid state fermentation

[0043] Inoculate Neurospora crassa in sterilized PDA solid medium for activation, and the activated strains are transferred to the above-mentioned solid fermentation medium for expanded culture;

[0044] Spread the ...

Embodiment 2

[0047] 1. Preparation of solid-state fermentation medium

[0048] First, the rice straw is crushed and passed through an 80-mesh sieve to obtain rice straw powder; then the rice straw powder is soaked in an acetic acid solution with a pH of 4.5 for acid treatment, and after 10 minutes of acid treatment, the rice straw powder is taken out and the volatile acid is evaporated. , the acidified rice straw powder of pH4.5 is obtained;

[0049] Add 3g of peptone to 1L of tap water, KH 2 PO 4 3g, MgSO 4 0.5g, NH 4 Cl1g, after stirring and dissolving, a liquid medium was prepared;

[0050] Mix the above-mentioned acidified rice straw powder with the above-mentioned liquid medium according to the weight ratio of 80:20 to prepare a solid-state fermentation medium;

[0051] 2. Solid state fermentation

[0052] Inoculate Neurospora crassa in sterilized PDA solid medium for activation, and the activated strains are transferred to the above-mentioned solid fermentation medium for expan...

Embodiment 3

[0055] 1. Preparation of solid-state fermentation medium

[0056] First crush the rice straw and pass through a 60-mesh sieve to obtain rice straw powder; then soak the rice straw powder in an acetic acid solution with a pH of 5.5 for acid treatment, take out the rice straw powder after 60 minutes of acid treatment, and evaporate the volatile acid , make the acidified rice straw powder of pH5.5;

[0057] Add 8g of peptone to 1L of tap water, KH 2 PO 4 1g, MgSO 4 1.5g, NH 4 Cl4g, after stirring and dissolving, a liquid medium is obtained;

[0058] Mix the above acidified rice straw powder with the above liquid medium according to the weight ratio of 60:40 to prepare a solid fermentation medium;

[0059] 2. Solid state fermentation

[0060] Inoculate Neurospora crassa in sterilized PDA solid medium for activation, and the activated strains are transferred to the above-mentioned solid fermentation medium for expanded culture;

[0061] Spread the solid-state fermentation me...

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Abstract

The invention discloses a method for preparing a protein feedstuff through straw solid-state fermentation by using neurospora crassa. According to the invention, straw is mixed with a liquid culture medium, such that a solid-state fermentation medium is prepared; and neurospora crassa is inoculated into the solid-state fermentation medium, and solid-state fermentation is carried out. The method provided by the invention has the advantages of single strain, simple process, short period, no three-waste production, and easy-to-control production process. Agricultural waste straw is adopted as the fermentation raw material, such that resource is saved, environment protection is promoted, and great economic benefit is provided. The protein feedstuff provided by the invention has high crude protein content, and is rich in lysine, threonine and vitamin B12. The feedstuff product is safe, and has no toxic or side effect. When the feedstuff is used in cooperation with other feedstuffs, a nutrition complementation effect is provided, feedstuff digestion and absorption are promoted, and feedstuff conversion rate is improved. The feedstuff can be used for partially replacing soybean meal or fishmeal in traditional feedstuffs, such that feedstuff cost can be reduced.

Description

technical field [0001] The invention relates to the technical field of feed, in particular to a method for preparing protein feed by solid-state fermentation of rice straw by Neurospora crassa, the prepared protein feed and applications thereof. Background technique [0002] Protein feed is a kind of feed with a crude protein content of more than 20% and a crude fiber content of less than 18% in dry matter. , methionine and tryptophan) and vitamins (such as vitamin B2 and B12), especially beneficial to the absorption and utilization of breeding animals. In addition, this type of feed also contains a special nutrient (growth factor), which can promote the utilization of nutrients by animals and stimulate the growth and reproduction of animals to varying degrees, which cannot be replaced by other nutrients. Commonly used vegetable protein feeds include soybean meal, cotton meal, rapeseed meal, peanut meal, etc. Commonly used animal protein feed includes fish meal, dairy produ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/00A23K1/14
CPCY02P60/87
Inventor 祝发明韩春元彭勇翟亚玲贾武利白晓婷同文江
Owner SHAANXI SHIYANG AGRI TECH
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