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A strain of Neurospora crassa and its application in the development of feed protein

A technology of Neurospora crassa and strains is applied in the field of Neurospora crassa and its mixed application, which can solve the problems of decreased utilization rate of feed protein raw materials, interference with the digestion and absorption of dietary nutrients, and high animal morbidity.

Active Publication Date: 2022-06-21
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the content of crude fiber in feed protein resources is high, and there are anti-nutritional factors such as xylan, cellulose, and β-glucan, which interfere with the digestion and absorption of dietary nutrients, reduce the nutritional value of feed, and lead to the utilization of feed protein raw materials. Declining rates, high disease rates in animals, etc.
And the amino acid composition of meal feed protein is not as ideal as that of animal source protein feed

Method used

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  • A strain of Neurospora crassa and its application in the development of feed protein
  • A strain of Neurospora crassa and its application in the development of feed protein
  • A strain of Neurospora crassa and its application in the development of feed protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: Acquisition of Neurospora crassa W3

[0016] 1. Obtainment of wild-type Neurospora crassa

[0017] In May 2021, it was isolated from rot wood microorganisms collected in Tangshan City, Hebei Province.

[0018] Separation process: scrape the decayed wood microorganisms, put them into a triangular flask containing 95 mL of sterile water and 10 glass beads, and shake at 30 °C and 180 rpm for 30 min. Take 1 mL of bacterial suspension for 10 -1 -10 -7 Serial concentration gradient dilution, then take 10 -5 , 10 -6 , 10 -7 Three dilutions were spread on plates of synthetic PDA medium and incubated upside down at 28°C for 4 d.

[0019] Purification: After the colony is formed on a medium plate with lignin as the only carbon source, select the strain with the fastest growth rate, pick the mycelium at the edge of the single colony on the PDA medium plate, and continue to cultivate at a constant temperature of 28°C. Until a pure colony of Neurospora crassa was...

Embodiment 2

[0055] Example 2: Growth or fermentation characteristics of N. crassa W3

[0056] When insoluble lignin / soluble lignin was used as the only carbon source, N. crassa W3 could grow normally, indicating that the strain has strong lignin degradation ability (such as figure 2 plate shown);

[0057] Fermentation medium: microcrystalline cellulose 33g / L, corn steep liquor dry powder 17g / L, KH 2 PO 4 1.60~1.72 g / L, (NH 4 ) 2 SO 4 2.6~3.0 g / L and MgSO 4 0.4~0.8 g / L. 24℃~28℃, pH 4.8~5.2, rotating speed 250~300 rpm, cultured for 5 days.

[0058] When N. crassa was fermented in a liquid shake flask for 5 days, its cellulase activity could reach 6.5 IU / ml, and its xylanase activity could reach 186.4 IU / ml; compared with the initial N. crassa wild strain, N. crassa The cellulase and xylanase activities of strain W3 were increased by 3.5 times and 12.4 times, respectively, indicating that this mutant strain has strong degrading ability of cellulose and hemicellulose;

[0059] ...

Embodiment 3

[0060] Example 3: Application of Fermented Meal Feed Protein

[0061] Seed medium: YPD medium: 1% glucose, 2% peptone, 1% yeast powder.

[0062] Wash the spore suspension concentration 10 from the plates of N. crassa W3 and A. niger 60B-3DW, respectively 7 1 / mL, add seed medium, culture temperature is 26 ℃ ~ 28 ℃, speed 180 ~ 200 rpm, culture for 24h.

[0063] Soybean meal, peanut meal, and cotton meal were mixed with bran at a ratio of 93:7 as fermentation substrates, and the inoculation amount of Aspergillus oryzae and Neurospora crassa was 10% (the proportion of Neurospora crassa W3 and Aspergillus niger 60B-3DW) 1:1), the water content was 60%, the culture temperature was 30 °C, and the fermentation was performed for 72 h.

[0064] The crude protein content and amino acid content of the meal materials before and after fermentation were determined respectively.

[0065] Crude protein GB / T 6432-1994 "Determination of crude protein in feed". Amino acid content was determi...

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Abstract

The invention belongs to the technical field of fermented meal feed and the application of single-cell protein from low-value raw material sources, and relates to a strain of Neurospora crassa and its mixed application. In the present invention, Neurospora crassa is a wild strain isolated from decaying wood microorganisms, and through ARTP mutagenesis, its ability to degrade biomass materials is improved, and a strain of Neurospora crassa W3 is obtained. Neurospora crassa W3 is shaken in a liquid state. After bottle fermentation for 5 days, the cellulase activity can reach 9.5IU / ml, and the xylanase activity can reach 186.4IU / ml. The invention also provides a method for producing single-cell protein by mixed solid-state fermentation of Neurospora crassa W3 and Aspergillus niger 60B‑3DW, which greatly improves the quality and nutritional value of meal feed. Therefore, the present invention can be applied industrially and has good industrialization prospect.

Description

technical field [0001] The invention belongs to the technical field of application of fermented meal feed and single-cell protein sources for producing low-value raw materials, and relates to a strain of Neurospora crassa and its mixed application. Background technique [0002] Insufficient supply of protein feed raw materials in my country coexists with the low utilization rate and large waste of traditional feed raw materials. On the one hand, my country's protein feed self-sufficiency rate is less than 50%, the dependence on foreign imports exceeds 80%, and the situation of "human and animal competition for food" and "subject to people" is becoming more and more serious. Very urgent; use biosynthesis technology to convert agricultural waste into unconventional protein raw materials, subverting the traditional agricultural protein production model. Feed protein derived from low-value raw materials can compete with soybean meal feed protein in many aspects such as protein u...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A23K10/12A23K10/37C12R1/645C12R1/685
CPCC12N1/14A23K10/12A23K10/37Y02P60/87
Inventor 吴信高乐
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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