Method for fermentation preparation of beta-renieratene by neurospora crassa

A carotene and production method technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of low yield and no economic value, and achieve high yield, low production cost and good product quality Effect

Inactive Publication Date: 2009-11-11
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them: Clark's hair mold: low yield, no economic value

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Mix bean dregs and rice bran evenly at a ratio of 2:1, moisten and steam the clinker, cool the clinker to room temperature and crush it quickly, inoculate Neurostria fortis, and culture it at 20°C-50°C for 1d-5d. Dry the culture medium (or collect spores) at 65°C to 80°C, add an appropriate amount of acetone to the dried culture medium, and use an ultrasonic breaker to break up the mature spores of the bacteria, and then use ethyl acetate to extract β- For carotene, combine the extracts and place them in a separatory funnel, wash with water twice, 8mL to 10mL each time, and discard the water layer. The organic layer was poured into a dry small Erlenmeyer flask from the top of the separatory funnel, and an appropriate amount of anhydrous magnesium sulfate or anhydrous sodium sulfate was added to dry overnight. Filter and concentrate by vacuum distillation to 1mL~2mL. The resulting concentrate is the β-carotene sample. Finally, the yield was determined by spectrophotome...

Embodiment 2

[0013] Mix bean dregs and rice bran evenly at a ratio of 3:1, then moisten and steam the material, cool the clinker to room temperature and quickly crush it, inoculate Neurostria fortis, and cultivate it at 40°C-50°C for 3d-5d, after the bacteria mature Dry the culture medium (or collect spores) at 65°C to 80°C, add an appropriate amount of acetone to the dried culture medium, and use an ultrasonic breaker to break up the mature spores of the bacteria, and then use ethyl acetate to extract β- Carotene, combined extracts and placed in a separatory funnel, washed twice with 15mL each time, discarded the water layer. The organic layer was poured into a dry small Erlenmeyer flask from the top of the separatory funnel, and an appropriate amount of anhydrous magnesium sulfate or anhydrous sodium sulfate was added to dry overnight. Filter and concentrate by vacuum distillation to 1mL~2mL. The resulting concentrate is the β-carotene sample. Finally, the yield was determined by spect...

Embodiment 3

[0015] Mix bean dregs and rice bran evenly at a ratio of 5:1, then moisten and steam the material, cool the clinker to about 37°C and crush it quickly, inoculate Neurostria fortis, and cultivate it at 20°C to 45°C for 3d to 5d. After maturity, dry the medium (or collect spores) at 65°C-80°C, add an appropriate amount of acetone to the dry medium, and use an ultrasonic breaker to break up the mature spores of the bacteria, and then use ethyl acetate to extract twice in the same way For β-carotene, combine the extracts and place them in a separatory funnel, wash with water twice, 20 mL each time, and discard the water layer. The organic layer was poured into a dry small Erlenmeyer flask from the top of the separatory funnel, and an appropriate amount of anhydrous magnesium sulfate or anhydrous sodium sulfate was added to dry overnight. Filter and concentrate by vacuum distillation to 1mL~2mL. The resulting concentrate is the β-carotene sample. Finally, the yield determined by ...

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Abstract

The invention relates to a method for the fermentation preparation of beta-renieratene by neurospora crassa, and is characterized in that the preparation method is as follows: (1) bean dregs and rice bran of which the mass ratio is 1-8:1 are weighed; after being uniformly mixed with each other, the bean dregs and the rice bran are moistened by water and then are steamed; (2) after steaming, the mixture is cooled to room temperature and is inoculated with the neurospora crassa, and the formed compound is cultured to be 1 to 5 days under the culture condition of 20 to 50 DEG C; (3) after fermentation is accomplished, fermented culture media is dried or spore is collected at the temperature from 65 to 80 DEG C, an ultrasonic cracking instrument is used for cracking mature thalli spore, and an organic solvent is used for extracting the beta-renieratene; and (4) the finished product of beta-renieratene can be obtained after the beta-renieratene is separated, purified and dried. The method has the advantages of: 1. high product quality and yield; 2. adoption of bean dregs and rice bran used as the culture media, available raw materials and low preparation cost; and 3. short fermentation period of neurospora crassa, high preparation efficiency, easy process control and high operability.

Description

technical field [0001] The invention relates to a method for fermenting and producing β-carotene by Neurostria crassa. Background technique [0002] β-carotene is mainly used as a natural pigment and a nutritional enhancer, which can prevent and treat vitamin A deficiency and dry eye disease in the human body. Studies in recent years have also shown that natural β-carotene has obvious effects in anti-cancer, anti-cancer and cardiovascular disease prevention. At present, the strains used to produce β-carotene at home and abroad mainly include Phycomyoces blakeskeanus, Blakesleatrispora and Rhodotorula. Among them: Clark's mustard mold: low yield, no economic value. Blakeslea trispora: It grows very rapidly and has high biomass. More than 50g of dry strains can be obtained per liter of fermentation broth after 48 hours of culture. It has a strong ability to produce β-carotene. The total carotene output is 1g / L after 5 days to 6 days of culture. Above, 80%-90% of which is β-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12R1/645
Inventor 邓泽元刘蓉贾才华李红艳范亚苇李静
Owner NANCHANG UNIV
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