Method for controlling mycelial morphology of Neurospora crassa and increasing output of cellulase

A technology of Neurospora crassa and cellulase, which is applied in the field of enzyme fermentation, can solve the problem that the shape of mycelium is not ideal, the cellulase yield of Neurospora crassa is low, and the high efficiency of Neurospora crassa cannot be achieved. Production of specific products and other issues, to achieve the effect of low cost, simple method, and increased secretion

Inactive Publication Date: 2017-11-24
NORTHWEST A & F UNIV
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  • Abstract
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Problems solved by technology

[0007] In order to solve the problem that the existing methods for changing the mycelial morphology of Neurospora crassa are not ideal for changing the mycelial morphology of Neurospora crassa, it is difficult to artificially and accurately control the mycelial morphology of Neurospora crassa fermentation, and it is impossible to efficiently produce specific products using Neurospora crassa demand, and the problem of low cellulase production of Neurospora crassa, the invention provides a method for controlling the mycelium morphology of Neurospora crassa fermentation and improving the production of cellulase

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  • Method for controlling mycelial morphology of Neurospora crassa and increasing output of cellulase
  • Method for controlling mycelial morphology of Neurospora crassa and increasing output of cellulase
  • Method for controlling mycelial morphology of Neurospora crassa and increasing output of cellulase

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Embodiment 1

[0033] The method for controlling the mycelium morphology of Neurospora crassa and improving the production of cellulase of the present embodiment comprises the following steps:

[0034] 1. Generation of conidia

[0035] Inoculate the conidia of Neurospora crassa FGSC1686 preserved on silica gel particles on the slant of sucrose solid medium, culture at 30°C for 3 days in the dark and light, and then continuously culture at 25°C for 5 days in the light, so that Produce mature conidia.

[0036] 2. Preparation of Seed Solution

[0037] Add PBS buffer solution (containing 0.1% Tween-80) to the slant of the above-mentioned sucrose solid medium, gently wash the mature conidia, and pour the washed conidia suspension into an Erlenmeyer flask containing an appropriate amount of glass beads ( Diameter is 2mm), vibrate 30min under the condition of 200rpm, remove remaining mycelium by 4 layers of gauze filtration, thereby can obtain dispersed, uniform spore suspension, utilize the bloo...

Embodiment 2

[0042] The difference between embodiment 2 and embodiment 1 is only that the addition of talcum powder is 0.3%, that is, the culture medium in the step 3 shake flask culture is 50mL microcrystalline fiber talcum powder liquid medium containing 0.3% talcum powder, and all the other are the same as those in the implementation Example 1 is the same.

Embodiment 3

[0044] The difference between embodiment 3 and embodiment 1 is that the final concentration of spores in the culture solution in the shake flask culture in step 3 reaches 8.9 × 10 6 / mL, the rest are the same as in Example 1.

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Abstract

The invention specifically relates to a method for controlling the mycelial morphology of Neurospora crassa and increasing the output of cellulase, belonging to the technical field of enzyme fermentation. The mycelial morphology of Neurospora crassa in fermentation is controlled by controlling the inoculation amount of the spores of Neurospora crassa and adding talcum powder, wherein the inoculation amount of the spores of Neurospora crassa is controlled to be 2.4 * 10<6>/ml to 8.9 * 10<6>/ml, and the addition amount of the talcum powder is 0 to 0.3%. The mycelial morphology of Neurospora crassa during fermentation is controlled by controlling the inoculation amount of the spores and the addition amount of the talcum powder, so the purpose of high-efficiency production of different desired products can be achieved; by controlling the inoculation amount of the spores of Neurospora crassa and the addition amount of the talcum powder, mycelia are allowed to be in a free and disperse state, and the output of cellulase is maximized; and the method provided by the invention has the advantages of simplicity, remarkable effect, low cost, good environmental compatibility and no subsequent recovery difficulties, and can be used for large-scale production.

Description

technical field [0001] The invention belongs to the technical field of enzyme fermentation, and in particular relates to a method for controlling the mycelium morphology of Neurospora crassa and improving the yield of cellulase. Background technique [0002] With the depletion of fossil fuels, looking for new substitutes for fossil fuels has far-reaching practical and historical significance, so it is also a hot research topic at home and abroad. Among many new energy researches, lignocellulosic energy leads the direction of biomass energy research due to its advantages of economy, environmental protection and sustainable development. The bottleneck problems of biomass energy development mainly include low yield of cellulase, high cost and low efficiency of enzymatic hydrolysis. How to screen and construct excellent lignocellulose-degrading microorganisms, improve the secretion efficiency of cellulase, and improve the enzymatic hydrolysis efficiency of cellulase are the pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/22C12N1/14C12N9/42C12R1/645
CPCC12N1/22C12N1/14C12N9/2437
Inventor 熊本涛韦林芳杨艳王一帆刘逸松郭仕婕张朵朵陈少林
Owner NORTHWEST A & F UNIV
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