Feeding microecologic preparation based on beer grains and rice bran meal
A technology of micro-ecological preparations and brewer's grains, applied in the application, animal feed, animal feed and other directions, can solve the problems of poor feeding effect, low preservation rate of probiotic bacteria enzymes, poor effect and so on
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Embodiment 1
[0053] The first phase of biological pretreatment fermentation
[0054] (1) Neurospora seeds and production medium.
[0055] Slant medium and plate medium: 200 g potato, 20 g sucrose, 20 g agar, in a constant volume of 1 L, pH 5.0, sterilized at 110°C for 30 min. Inoculate at 30°C and culture for 7-10 days.
[0056] Seed liquid medium: KH 2 PO 4 2g, MgSO 4 0.3g, CaCl 2 0.3g, peptone 5g, yeast extract 3g, wort 3g, FeSO 4 ·7H 2 O 0.005g, ZnSO 4 0.0014g, MnSO 4 .4H 2 O 0.0016g, CoCl 2 0.002 g, dilute to 1 L, pH 5.0. Add 100 ml culture medium to a 500 ml Erlenmeyer flask, and sterilize at 110°C for 30 minutes.
[0057] Solid-state fermentation production seed medium and production medium: crushed wet brewer's grains accounted for 67% of the total mass of the medium, rice bran meal accounted for 28%, ammonium nitrate accounted for 2%, urea accounted for 1%, calcium carbonate accounted for 1%, diphosphate Potassium hydrogen 1%, magnesium sulfate 0.1%, the final water...
Embodiment 2
[0061] The second stage of fermentation
[0062] (1) Bacillus culture medium
[0063]Bacillus slant and plate culture medium: peptone 10g, beef extract powder 5g, sodium chloride 5g, agar 15g, glucose 20g, distilled water 1000ml, final pH7.0±0.2. Sterilize at 110°C for 30min.
[0064] Bacillus shake flask seed medium and seed tank medium: beef extract 5.0g / L, peptone 20.0g / L, glucose 5.0g / L, FeCl 2 6H2O 0.07g / L, MnC1 2 ·7H 2 O 0.01g / L, MgSO 4 ·7H 2 O 0.15g / L, pH 7.0, sterilized at 110°C for 30min.
[0065] (2) Yeast medium
[0066] Yeast slant medium and plate culture medium (100 ml): 2 g glucose, 1 g yeast extract, 2 g peptone, 2 g agar, pH value about 6.0.
[0067] Shake flask seed medium and seed tank medium: the composition is the same as that of the slant medium, without agar.
[0068] (3) The second-stage fermentation medium is composed of the solid bacterial agent pretreated by the first-stage fermentation plus 15% rice bran and 5% soybean meal, with a water con...
Embodiment 3
[0076] The third stage of fermentation
[0077] (1) Lactic acid bacteria medium
[0078] MRS slant medium (g / L): peptone 10, yeast powder 5, beef extract 5, glucose 20, diammonium citrate 2, Tween 80 1.0 ml, sodium acetate 25, K 2 HPO 4 2. MgSO 4 7H 2 O 0.58, MnSO 4 4H 2 O 0.25, Agar 20, pH 7.0.
[0079] Shake flask and seed tank lactic acid bacteria seed medium (g / L): 2.25% soybean oligosaccharides, 2.00% glucose, 1.25% peptone, 1.25% yeast powder, 6.50% tomato juice, 80.10% Tween, 0.20 dipotassium hydrogen phosphate %. pH 6.5, 200 ml in a 1L Erlenmeyer flask.
[0080] After the above medium was prepared, all of them were autoclaved at 115°C for 30 minutes.
[0081] (2) Bifidobacteria ( Bifidobacterium bifidum , CGMCC 1.2477) medium
[0082] MRS slant medium (g / L): peptone 10, yeast powder 5, beef extract 5, glucose 20, diammonium citrate 2, Tween 80 1.0 ml, sodium acetate 25, K 2 HPO 4 2. MgSO 4 7H 2 O 0.58, MnSO 4 4H 2 O 0.25, Agar 20, pH 7.0.
[0083] ...
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