Feeding microecologic preparation based on beer grains and rice bran meal

A technology of micro-ecological preparations and brewer's grains, applied in the application, animal feed, animal feed and other directions, can solve the problems of poor feeding effect, low preservation rate of probiotic bacteria enzymes, poor effect and so on

Active Publication Date: 2012-09-26
JIANGSU UNIV
View PDF12 Cites 24 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, after rice bran is properly treated, the feeding effect may be better. The published patent CN102334611A is inserted into Bacillus natto and yeast, and the disclosed patent CN102356815A is inserted into enzyme bacteria, Bacillus subtilis, yeast and Lactobacillus. The lignocellulose degradation ability of the latter is limited, a...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The first phase of biological pretreatment fermentation

[0054] (1) Neurospora seeds and production medium.

[0055] Slant medium and plate medium: 200 g potato, 20 g sucrose, 20 g agar, in a constant volume of 1 L, pH 5.0, sterilized at 110°C for 30 min. Inoculate at 30°C and culture for 7-10 days.

[0056] Seed liquid medium: KH 2 PO 4 2g, MgSO 4 0.3g, CaCl 2 0.3g, peptone 5g, yeast extract 3g, wort 3g, FeSO 4 ·7H 2 O 0.005g, ZnSO 4 0.0014g, MnSO 4 .4H 2 O 0.0016g, CoCl 2 0.002 g, dilute to 1 L, pH 5.0. Add 100 ml culture medium to a 500 ml Erlenmeyer flask, and sterilize at 110°C for 30 minutes.

[0057] Solid-state fermentation production seed medium and production medium: crushed wet brewer's grains accounted for 67% of the total mass of the medium, rice bran meal accounted for 28%, ammonium nitrate accounted for 2%, urea accounted for 1%, calcium carbonate accounted for 1%, diphosphate Potassium hydrogen 1%, magnesium sulfate 0.1%, the final water...

Embodiment 2

[0061] The second stage of fermentation

[0062] (1) Bacillus culture medium

[0063]Bacillus slant and plate culture medium: peptone 10g, beef extract powder 5g, sodium chloride 5g, agar 15g, glucose 20g, distilled water 1000ml, final pH7.0±0.2. Sterilize at 110°C for 30min.

[0064] Bacillus shake flask seed medium and seed tank medium: beef extract 5.0g / L, peptone 20.0g / L, glucose 5.0g / L, FeCl 2 6H2O 0.07g / L, MnC1 2 ·7H 2 O 0.01g / L, MgSO 4 ·7H 2 O 0.15g / L, pH 7.0, sterilized at 110°C for 30min.

[0065] (2) Yeast medium

[0066] Yeast slant medium and plate culture medium (100 ml): 2 g glucose, 1 g yeast extract, 2 g peptone, 2 g agar, pH value about 6.0.

[0067] Shake flask seed medium and seed tank medium: the composition is the same as that of the slant medium, without agar.

[0068] (3) The second-stage fermentation medium is composed of the solid bacterial agent pretreated by the first-stage fermentation plus 15% rice bran and 5% soybean meal, with a water con...

Embodiment 3

[0076] The third stage of fermentation

[0077] (1) Lactic acid bacteria medium

[0078] MRS slant medium (g / L): peptone 10, yeast powder 5, beef extract 5, glucose 20, diammonium citrate 2, Tween 80 1.0 ml, sodium acetate 25, K 2 HPO 4 2. MgSO 4 7H 2 O 0.58, MnSO 4 4H 2 O 0.25, Agar 20, pH 7.0.

[0079] Shake flask and seed tank lactic acid bacteria seed medium (g / L): 2.25% soybean oligosaccharides, 2.00% glucose, 1.25% peptone, 1.25% yeast powder, 6.50% tomato juice, 80.10% Tween, 0.20 dipotassium hydrogen phosphate %. pH 6.5, 200 ml in a 1L Erlenmeyer flask.

[0080] After the above medium was prepared, all of them were autoclaved at 115°C for 30 minutes.

[0081] (2) Bifidobacteria ( Bifidobacterium bifidum , CGMCC 1.2477) medium

[0082] MRS slant medium (g / L): peptone 10, yeast powder 5, beef extract 5, glucose 20, diammonium citrate 2, Tween 80 1.0 ml, sodium acetate 25, K 2 HPO 4 2. MgSO 4 7H 2 O 0.58, MnSO 4 4H 2 O 0.25, Agar 20, pH 7.0.

[0083] ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a feeding microecologic preparation which has high efficiency, is very low in cost, and is formed by performing three stages of probiotics fermentation to beer grains and rice bran meal, and is also called microbial forage additive. The feeding microecologic preparation is characterized in that after beer grains and rice bran meal are subjected to the first stage of fermentation preprocessing through neurospora, lignin, cellulose and the like are sufficiently degraded; part of rice bran meal and a small amount of soya bean meal are added to be subjected to the second stage of fermentation through bacillus and microzyme; and nutrient substances generated when waste is degraded by mould and nutrient substances generated when mould is dismembered are absorbed; and then part of rice bran meal and a small amount of soya bean meal are added to be finally subjected to the third stage of anaerobic fermentation through lactobacillus, bifidobacterium and the like in a one way membrane anaerobic bag, so that finished products are obtained. The feeding microecologic preparation has the advantages that solid material is not required to be subjected to high temperature sterilization, the finished products are not required to be subjected to drying process, the quality guarantee period is more than one year, the amount of additive reaches 5 to 30 percent, the amount of active probiotics is high, the amount of enzyme is abundant, the amount of bacterials is very little, the feeding effect is obvious, and the feeding microecologic preparation has obvious ecological and social benefits.

Description

technical field [0001] The invention belongs to the field of microecological preparations for feeding, in particular to a novel microbial feed additive with high efficiency and low cost based on fibrous brewing waste. Background technique [0002] China is already the world's largest beer country, with an annual output of more than 10 million tons of beer's grains, which are rich in protein (25-35%), cellulose (14-25%), lipids (6-10%), vitamins and other nutrients, but its high moisture content makes it difficult to store, and once unsalable, it is easy to rot and rot and affect the environment. At present, some domestic beer production enterprises directly dry the brewer's grains and sell them as feed, which not only consumes a lot of energy, but also the non-protein nitrogen and inorganic nitrogen in this feed cannot be effectively used by animals, and the cellulose also hinders the absorption of nutrients. . Therefore, the reasonable development and utilization of brewe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A23K1/00A23K1/16A23K1/175
Inventor 陈华友杨胜利李萍萍谢永明施海峰倪忠谭小力崔凤杰崔恒林齐向辉王浩森
Owner JIANGSU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products