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Preparation method of biological pigment by double-strain fermentation with wastewater rich in amino acids as base material

A biopigment and amino acid technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of low yield and high fermentation cost, and achieve high yield, high-efficiency expression intensity, and strong ecological acceptability.

Active Publication Date: 2019-07-19
优贝佳(青岛)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This process still has the problems of high fermentation cost and low yield.

Method used

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  • Preparation method of biological pigment by double-strain fermentation with wastewater rich in amino acids as base material
  • Preparation method of biological pigment by double-strain fermentation with wastewater rich in amino acids as base material
  • Preparation method of biological pigment by double-strain fermentation with wastewater rich in amino acids as base material

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Experimental program
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Effect test

Embodiment 1

[0032] (1) Preparation of seed medium

[0033] Neurospora crassa (Neurospora crassa ATCC10815) and black fungus (Auricularia auricula) were inoculated on PDA plates from the slant preservation tubes and cultured at 28°C for 10 days, and the colony morphology was shown in the figure ( figure 1 ). During the 10-day cultivation process, the seed medium was prepared;

[0034] Seed medium: 200 ml of milk factory waste water (after filtering to remove impurities, the total content of main components amino acid and protein 0.6%, oil 0.02%), add glucose 6g, peptone 4g, CaCl 2 0.07g, vitamin B 0.1g, vitamin C 0.1g, adjust pH 7.0-8.0. The culture medium was sterilized at 115°C for 30 min under high temperature and high pressure.

[0035] (2) Preparation of primary seed solution

[0036] On the PDA plate, 8 pieces of 0.5mm × 0.5mm bacterium blocks were picked, respectively inoculated in the seed culture medium of 200mL, and 26 ℃, 150rpm shake flask cultured for 3 days, as the first-...

Embodiment 2

[0046] (1) Preparation of seed medium

[0047] Neurospora crassa (Neurospora crassa ATCC10815) and black fungus (Auricularia auricula) were inoculated on PDA plates from slant preservation tubes and cultured at 28°C for 10 days. During the 10-day cultivation process, the seed medium was prepared;

[0048] Seed medium: 2000 ml of waste water rich in amino acids, milk factory waste water (after filtering to remove impurities, the total content of amino acids and protein in the main components is 0.6%, and oil 0.02%), add glucose 60g, peptone 30g, CaCl 2 0.6g, vitamin B 0.5g, vitamin C 0.5g, adjust the pH to 7.0-8.0. The medium was sterilized at 115°C for 30 min under high temperature and high pressure.

[0049] (2) Preparation of primary seed solution

[0050] Pick 20 pieces of 0.5mm×0.5mm bacterial pieces on the PDA plate, inoculate them in 2000mL seed culture medium respectively, and cultivate them at 26°C and 150rpm for 4 days as the primary seed solution.

[0051] (3) P...

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Abstract

The invention discloses a preparation method of a biological pigment by double-strain fermentation with wastewater rich in amino acids as a base material. In the preparation method, Neurospora crassaand Auricularia auricula are used as fermentation strains, and amino acid-rich wastewater is used to prepare a fermentation medium, oscillating fermentation culture is carried out at 25-32 DEG C, andthe obtained fermentation broth is subjected to extracting and concentrating, thereby obtaining the biological pigment. According to the invention, the wastewater rich in amino acids is utilized to prepare the fermentation medium, and then double-strain coupling fermentation is utilized to produce the pigment; meanwhile, the treatment of wastewater rich in amino acids and the application of wastewater resource are efficiently combined, reducing the cost of producing the biological pigment. The produced biological pigment has the characteristics of high yield, environmental protection and strong ecological acceptability, and has a good staining effect on proteins.

Description

technical field [0001] The invention relates to a method for preparing biological pigments by double-bacteria fermentation using waste water rich in amino acids as a base material, and belongs to the field of microbial fermentation. Background technique [0002] It is the need of current development to greatly improve the large-scale production and application level of food and feed additives such as bio-pigments and bio-flavors. In recent years, countries around the world have successively formulated regulations to eliminate most toxic chemically synthesized pigments. Those natural pigments that have coloring functions, nutrition and health effects, and can endow food with new functions will have broad market prospects. The annual trade volume of natural pigments in the world is 1.3 billion US dollars, the annual demand is about 15,000 tons, and it is increasing at an annual rate of 11%. At present, most varieties of natural pigments come from plant extraction, but their d...

Claims

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Application Information

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IPC IPC(8): C12P1/02C12N1/14G01N33/68C12R1/645
CPCC12N1/14C12P1/02C12N1/145C12R2001/645G01N33/68
Inventor 夏文杰王强宋凯凯
Owner 优贝佳(青岛)生物科技有限公司
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