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Pretreatment method of straw biological feed raw material

A straw biological feed and pretreatment technology, which is applied to the pretreatment of straw biological feed raw materials, the preparation of biological feed raw materials that produce true protein by fermentation, straw alkali treatment, and the field of biological detoxification, which can solve the problem that the extraction is not necessarily complete and the cost is high. , flammability and other issues, to achieve the effect of increasing nutritional value, low price, and reducing concentration

Active Publication Date: 2018-11-16
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extraction is not necessarily thorough, and some commonly used organic solvents are flammable and toxic, and the cost of large-scale extraction is relatively high
Chemical methods include precipitating toxic substances, or ionizing certain inhibitors under certain pH conditions, thereby changing the toxicity of compounds, but there are certain limitations to the removal of toxic compounds, and the cost is generally high

Method used

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  • Pretreatment method of straw biological feed raw material
  • Pretreatment method of straw biological feed raw material
  • Pretreatment method of straw biological feed raw material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A pretreatment method for straw biological feed raw materials, the method comprises the following steps:

[0034] (1) Crush: Crush the corn stalks into 0.1mm particles;

[0035] (2) Add Ca(OH) according to the mass ratio of straw to calcium hydroxide 10:1 (g:g) 2 , with a water content of 80%, stir well, and treat at 80°C for 2 hours. Weighed part of the semi-solid straw and dissolved it in water, extracted with dichloromethane to determine the phenolic substances in it, see the chromatographic peak diagram in the attached figure 2 , it can be seen from the figure that vanillin, p-hydroxybenzaldehyde and ferulic acid were released to a large extent after the straw was treated with alkali at 80°C.

[0036] (3) Adjust the pH of the straw mixture to 5.0;

[0037] (4) Cu in straw mixture 2+ The final concentration is 30mg / L, Mn 2+ The final concentration is 30mg / L, the amount of laccase added is 0.1%, the activity is 10000U / mL, the detoxification temperature of laccase ...

Embodiment 2

[0042] Using the same conditions as in Example 1, the detoxification time of Phanerochaete chrysosporium was extended to 36 hours during the detoxification process. After detoxification, the content of phenolic inhibitors was determined by high performance liquid chromatography. The results showed that the degradation rates of vanillin, p-hydroxybenzaldehyde, ferulic acid, 2-methoxy-4-vinylphenol and salicylic acid were respectively 90.12%, 88.73%, 98.85%, 86.37%, 78.33%. The peak diagram of phenolic substances in the liquid components is shown in the appendix Figure 4 . It can be seen from the figure that vanillin, p-hydroxybenzaldehyde and ferulic acid have all been converted to a large extent, indicating that further extending the detoxification time of Phanerochaete chrysosporium can achieve a better detoxification effect.

[0043] The fungal fermentation conditions were the same as Example 1, and the true protein content increased by 2.64 times after Neurospora ferment...

Embodiment 3

[0045] The same conditions as in Example 1 were adopted, but the sequential detoxification of laccase and Phanerochaete chrysosporium was changed to simultaneous detoxification for 24 hours. After detoxification, the content of phenolic inhibitors was determined by high performance liquid chromatography. The results showed that the degradation rates of vanillin, p-hydroxybenzaldehyde, ferulic acid, 2-methoxy-4-vinylphenol and salicylic acid were respectively 58.37%, 64.61%, 83.67%, 30.70%, 20.42%. The peak diagram of phenolic substances in the liquid components is shown in the appendix Figure 5 . It can be seen from the figure that the three main phenolic substances have been transformed to a greater extent; the detoxification of laccase and Phanerochaete chrysosporium has a certain synergistic effect.

[0046] The fungal fermentation conditions were the same as Example 1, and the true protein content increased by 1.93 times after Neurospora fermentation.

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Abstract

The present invention relates to a pretreatment method of a straw biological feed raw material and belongs to the technical field of biological feeds. Straw is used as a main raw material; firstly, alkali liquid is used to pretreat crushed corn straw; then a laccase and white rot fungus combined detoxification method is used to remove various phenolic compounds inhibiting fermentation micro-organism flora generated during the pretreatment process; and then neurospora is inoculated in the detoxified corn straw for fermentation to obtain the straw feed raw material which is reduced in lignin andsignificantly increased in true proteins. The straw biological feed raw material is rich in nutrients and easy to digest, and provides a new way for the high-efficient feed application of the straw.

Description

technical field [0001] The invention relates to a pretreatment method of straw biological feed raw materials, in particular to a preparation method of biological feed raw materials including straw alkali treatment, biological method detoxification and fermentation to produce true protein, belonging to the technical field of biological feed. Background technique [0002] The cell wall of corn stover is composed of lignin, hemicellulose, and cellulose tightly connected, and this tight network structure can resist biomass saccharification. In order to change this complex structure and facilitate enzymatic hydrolysis and subsequent fermentation steps, pretreatment becomes necessary. Pretreatment is the basic process for decomposing refractory biomass. Appropriate pretreatment methods can increase the porosity of straw, expand the interaction area between enzymes and substrates, and finally improve the degradation rate of corn straw. Among them, lignin is the main component of b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/37A23K20/24A23K20/105A23K20/20A23K20/163A23K10/12C12P1/02C12R1/645
CPCA23K10/12A23K10/37A23K20/105A23K20/163A23K20/20A23K20/24C12P1/02C12N1/145C12R2001/645Y02P60/87
Inventor 陈华友吴邦国王洪成李文跃崔凤杰齐向辉倪忠
Owner JIANGSU UNIV
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