Edible fungus strain preparation method capable of preventing damp cotton plugs in strain bottles or culture bags

Abstract

Disclosed is an edible fungus strain preparation method capable of preventing damp cotton plugs in strain bottles or culture bags. The method is characterized that during the production of the stock strain or culture strain, the process comprises preparing raw materials, which comprises preparing wheat grains, sawdust and cottonseed shell as culture medium for stock strain, or preparing wheat grains, sawdust and cottonseed shell as culture medium for culture strain, and preparing moisture-proof covers and plenty of cotton plugs for later use; feeding the culture medium into strain bottles or culture bags with conventional methods, inserting cotton plugs into bottle mouths or opening of the culture bags, and covering the moisture-proof covers at the bottle mouths or bag openings to form moisture-proof cover layers for preventing cotton plugs from being moistened, sterilizing the culture medium by atmospheric pressure steam sterilization or high pressure steam sterilization; and inoculating and culturing to obtain stock strain or culture strain. The method of the invention can completely prevent moisture absorption phenomenon, the operation is simple, the moisture-proof covers can be repeatedly used, the use cost is low, the problem of moisture absorption of the cotton plugs can be well solved, Neurospora growth on the cotton plugs after inoculation can be prevented, and contamination of the fungus strains is avoided.

Description

(1) Technical field [0001] The invention relates to a production technology of edible fungus strains, in particular to a production method of edible fungus strains (referring to original species or cultivated species) which can prevent the cotton plugs in the strain bottles or culture bags from getting wet. (two), background technology [0002] As we all know, the key to the success of artificial cultivation of edible fungus is the strain. Only excellent strains can achieve high quality and high yield. species; the second stage is to produce the original species, that is, to use the mycelium of the mother species to insert into the original seed medium, and to expand and reproduce the original species (also known as the secondary species); the third stage is the production and cultivation It is not enough to have the original species. In the third stage, it will be expanded to cultivated species, that is, "third-level species". strains put into production). Because in the ...

AI Technical Summary

Problems solved by technology

Because in the process of making large-scale strains, as when the culture medium of the original species or cultivars is packed into the strain bottle, or the culture medium of the cultivars is packed into the culture bag for strain production, And in the operation process of inoculation (sowing) etc., it is difficult to achieve a real aseptic operation state. In order to reduce the contamination rate of strains and improve the yield of strains, in each stage of strain production, people have adopted many methods that can reduce the contamination rate of strains. Measures or methods of strain contamination rate, such as currently in the production process of strains (referring to original species or cultivated species), people also usually adopt the method of steam sterilizing the culture medium, that is, in the strain bottle (such as the original Seed bottle) or the medium is loaded into the culture bag used for the production of strains, and a cotton plug is inserted into the mouth of the bottle or the mouth of the culture bag. When using normal pressure or high-pressure steam sterilization, it often occurs Wet phenomenon of cotton plugs (the specific reason is that after high-temperature steam sterilization and cooling, condensed water will be generated at the mouth of the bottle (or at the mouth of the culture bag), making the cotton plugs wet), in order to solve this problem, generally use Use waste newspapers and other materials to wrap cotton plugs to prevent the cotton from getting wet and collect water. This method can achieve a certain moisture-proof effect, but because of the incomplete moisture-proof effect, it often produces bacteria pollution (grown on cotton plugs) Neurospora rubrum), seriously restricts and affects the survival rate and yield of strain production, reaching 50% pollution, even up to more than 100%

It also seriously restricts and affects the production efficiency

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