Microbial fermenting agent

A microbial starter, starter technology, applied in the directions of microorganisms, microorganisms, biochemical equipment and methods, etc., can solve problems such as low efficiency, and achieve the effect of improving degradation efficiency and promoting crop growth

Inactive Publication Date: 2006-12-20
NANJING NORMAL UNIVERSITY
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Problems solved by technology

[0007] In order to overcome the inefficiency of existing microbial starters in material fermentation, the present invention provides a microbial starter that effectively solves the problems of degradation of various macromolecules in materials and rapid temperature rise of materials; on this basis, also Further provide a kind of compound microorganism fermenting agent that has strong ability to degrade cellulosic substances, use bacterial strains that produce cellulase, hemicellulase and other cellulosic substances degrading enzymes in the fermenting agent, especially Aspergillus, Penicillium , Neurospora, etc., better solve the degradation problem of fiber materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Raw material: Cow dung from a dairy factory. Medium: solid medium used for mold fermentation in starter: 70g straw, 30g bran, 2g ammonium sulfate, 0.26g sodium dihydrogen phosphate, 0.51g magnesium sulfate, 200ml water, natural pH. Incline activation medium: PDA medium, broth medium; liquid culture medium for expansion: PDA medium (to cultivate yeast), broth medium (to cultivate bacteria), Gao's medium (to cultivate actinomycetes).

[0039] Preparation of starter: insert the slant strains into the corresponding culture medium, carry out liquid or solid culture under different conditions, and make finished starter products with different formulas in proportion after the cultivation is completed.

[0040]Cow dung 600kg is heaped into conical shape, and the initial moisture of fermentation is controlled at 60% (wet basis), adds 600g of leavening agent of formula of the present invention, mixes. During the composting process, the changes in air temperature and compost temp...

Embodiment 2

[0045] Embodiment 2: total nutrient after compost fermentation, the mensuration of organic matter and pH value

[0046] After the compost of the above three formulas was fermented for 16 days, its total nitrogen, total phosphorus, total potassium, organic matter and pH value were determined according to the agricultural industry standard NY525-2002 "Organic Fertilizer" of the People's Republic of China. The results are shown in Table 1.

[0047] Bacteria

[0048] According to the Ministry of Agriculture's organic fertilizer industry standard (NY525-2002), it is required that the total nutrient (nitrogen + phosphorus pentoxide + potassium oxide) of the organic fertilizer should be ≥ 4.0%, the organic matter content should be ≥ 30%, and the pH should be between 5.5 and 8.0. It can be seen from Table 1 that the composts of the three formulas have reached the standard in terms of total nutrient, organic matter and pH value, so they can be directly used as organic fertili...

Embodiment 3

[0049] Embodiment 3: the mensuration of water content after compost fermentation

[0050] The water content of fresh cow dung is very high, and the water content problem in cow dung is related to whether the microbial fermentation can be carried out smoothly and whether the final product can reach the water content standard of organic fertilizer (the water content standard of organic fertilizer requires ≤20%). In this experiment, the water content was adjusted to about 120% (dry basis) by adding some other water-absorbing agents such as dry cow dung or sawdust. After 16 days of fermentation in the form of compost, the water had been reduced to varying degrees. After the fermentation, the water content of the material was measured The result is as figure 2 shown.

[0051] Depend on figure 2 It can be seen that although the moisture content of cow dung (CK) without bacterial agent treatment has decreased after fermentation, the moisture content is still relatively high (71.3...

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Abstract

A microbial leaven used for preparing organic or microbial fertilizer from organic waste is prepared from at least two of nine microbes. Aspergillus, Sporotrichun, Mucoraceae, Penicillium sp, Ceotrichum sp., Neurospora, Bacillus, Saccharomyces and Streptomyces sp.

Description

technical field [0001] The invention relates to a microbial fermentation agent, in particular to a microbial fermentation agent for treating biologically sourced organic matter, especially livestock and poultry farming waste, crop stalks and the like. Background technique [0002] The realization of ecological agriculture, green agriculture, and organic agriculture is closely related to the development of biotechnology, and the application of microorganisms is one of its key points. The large amount of organic waste produced in my country every year seriously restricts the sustainable development of agriculture, livestock and poultry breeding, and edible fungus cultivation. [0003] The development of ecological agriculture and organic agriculture is an important way for the development of modern agriculture, and the application of organic fertilizer is an important aspect of improving ecology and developing modern agriculture. According to rough statistics, my country prod...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C12N1/14C12N1/20C12N1/16
Inventor 陈育如骆跃军李雪梅刘媛
Owner NANJING NORMAL UNIVERSITY
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