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Bactericide for suppressing growth of neurospora and preparation method thereof

A bactericide, Alternaria technology, applied in the field of microorganisms, can solve the problems of weak bactericidal effect of Alternaria, pollute the environment, economic losses, etc., and achieve the effects of preventing and controlling Alternaria pollution, good bactericidal effect, and convenient use.

Inactive Publication Date: 2011-01-12
SHANGHAI ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Once the bacteria bag is contaminated, it is difficult to completely remove it, often causing the entire batch of bacteria or bacteria bags to be scrapped, resulting in great economic losses
[0005] Currently commonly used neurospora fungicides are chlorothalonil and carbendazim, but these two fungicides have the following defects: strong toxicity, great harm to production species, polluting the environment, and weak bactericidal effect on neurospora

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Preparation of Neurospora fungicide 1 and detection of fungicidal activity

[0029] Prepare liquid medium: beef extract 3 g, peptone 10 g, NaC 15 g, agar 20 g, water 1000 mL, pH 7.0-7.2; put Pseudomonas putida into the liquid medium according to the inoculum size of 5%, put Cultivate in a constant temperature shaker at 37°C, with a rotation speed of 120rmp / min, and shake for 4 days; centrifuge the cultured bacterial solution at 4000rmp / min for 15 minutes, and take the supernatant. After repeating 3 times, take the supernatant and vacuum filter it. As a fungicide 1. Store in a sealed container at 4°C.

[0030] Streak fungicide 1 on both sides of a 90mm LB plate 2cm away from the center, inoculate a white Neurospora plate and a red Neurospora plate in the center respectively, culture at 28°C for 3 days, observe the growth of Neurospora, and compare with the blank Control comparison.

Embodiment 2

[0031] Example 2 Preparation of Neurospora fungicide 2 and detection of its fungicidal activity

[0032] The Neurospora fungicide 1 prepared in Example 1 was filtered through a 0.22 μm microporous membrane, sealed and stored at 4° C., and used as fungicide 2.

[0033] After dissolving 100ml PDA, add 11ml of fungicide 2 (10%); each 100ml of PDA was poured into six dishes, and inoculated with Neurospora rubrum, Neurospora albicans, and blank control respectively; PDA plates without any treatment were inoculated with Neurospora rubrum (R) and Neurospora albicans (W).

Embodiment 3

[0034] Example 3 Preparation of Neurospora fungicide 3 and detection of its fungicidal activity

[0035] Filter the Neurospora fungicide 1 prepared in Example 1 with a 10 μm microporous membrane, concentrate it with a 45° C. rotary evaporator until the OD value of the bacterial solution is 0.1, filter it with a 0.22 μm microporous membrane, and store it in a sealed container at 4° C. Agent 3.

[0036] After dissolving 80ml of PDA, add 20ml of fungicide 3 (20%); each 100ml of PDA was poured into six dishes, and inoculated with Neurospora rubrum, Neurospora albicans, and blank control respectively; PDA plates without any treatment were inoculated with Neurospora rubrum (R) and Neurospora albicans (W).

[0037] The fungicides in Example 1, Example 2 and Example 3 can all effectively kill Neurospora, and their bactericidal rates to Neurospora rubrum and Neurospora albicans are shown in the table below.

[0038]

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PUM

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Abstract

The invention discloses a bactericide for suppressing growth of neurospora. The neurospora bactericide is prepared by performing liquid culture on a pseudomonas putida strain and centrifugally extracting supernatant from the bacterial solution. The neurospora bactericide of the invention has the characteristics of good sterilization effect, safety and the like.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a fungicide for inhibiting the growth of Neurospora and a preparation method thereof. Background technique [0002] Neurospora is a tenacious, fast-growing aerial mold that mainly harms various edible fungus strains, and also occurs during cultivation. It harms the mycelium and fruiting bodies of edible fungi, and is extremely difficult to control. Although there are chemical agents to prevent and control , but the effect is not good, and the toxicity is high, so far no microorganisms or their metabolites that can effectively inhibit the growth of Neurospora have been found. [0003] Neurospora is widely distributed in natural soil, gramineous plants and starch-rich foods, and can produce a large number of conidia, which are spread by airflow. The environment of the cultivation room is unhygienic; the autoclaving of the bacterial bag is not complete; the tampon is damp ...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01P3/00
Inventor 郭倩林娓娓李玉周峰于海龙王瑞娟
Owner SHANGHAI ACAD OF AGRI SCI
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