Application of chlorogenic acid in prevention and treatment of soybean root rot

Abstract

The invention discloses application of chlorogenic acid in prevention and treatment of soybean root rot, and relates to the technical field of crop protection. Chlorogenic acid has prevention and treatment effects on soybean root rot caused by four pathogenic bacteria, the prevention and treatment effects on the root rot caused by fusarium tripteroides and fusarium oxysporum are best, and the prevention and treatment effects on the root rot caused by phomopsis and phytophthora sojae are relatively poor; according to the potting control effect, the treatment of spraying the metabolite in advance promotes the growth of soybean seedlings along with the increase of the concentration, the plant height, root length and other physiological indexes of the soybean seedlings are all higher than those of contrast treatment without inoculation of pathogenic bacteria, the disease index is obviously reduced, and the control effect of 200 mu g / mL chlorogenic acid treatment at 20 days reaches 60% or above and is slightly higher than that of a bactericide-methyl fludioxonil. The chlorogenic acid has excellent prevention and treatment potential on soybean root rot, and has a wide development prospect.

Description

Technical Field

[0001] The present invention relates to the technical field of crop protection, and more particularly to the application of chlorogenic acid in preventing and treating soybean root rot. Background Art

[0002] At present, soybean root rot is one of the most serious limiting factors affecting the healthy growth and yield increase of soybeans. It can occur from the seedling stage to the pod-setting stage, mainly harming the roots of soybeans, resulting in underdeveloped root systems, few nodules, weak growth of the aboveground parts, easy lodging, and easy occurrence of diseases and insect pests. In severe cases, the plants dry up and die. . When the disease is mild, the impact on yield is small, but severe disease will lead to a reduction of more than half of the yield, while affecting the quality of soybeans and reducing the oil content. So far, 65 soybean root rot pathogens have been discovered in the world, and about 30 species have been reported in China. Among them, Phytophthora sojae, Fusarium oxysporum, Phomopsis sphaeroides and Fusarium trilineatum are all dominant populations in different soybean producing areas. The use of chemical fungicides for prevention and control is still the best prevention and control strategy at present.

[0003] However, due to the complex population structure of soybean root rot, chemical fungicides can easily lead to the development of drug resistance, the microecological environment deteriorates, the prevention and control effect is not ideal, and it seriously affects the healthy and sustainable development of the soybean planting industry.

[0004] Therefore, exploring and developing new soybean root rot prevention and treatment preparations is an urgent problem to be solved by those skilled in the art. Summary of the invention

[0005] In view of this, the present invention provides the use of chlorogenic acid in preventing and treating soybean root rot.

[0006] In order to achieve the above object, the present invention adopts the following technical solution:

[0007] Application of chlorogenic acid in preventing and controlling soybean root rot

[0008] Furthermore, the pathogenic bacteria of soybean root rot include: Phomopsis spp., Fusarium trilineata, Fusarium oxysporum, and Phytophthora sojae.

[0009] A composition for preventing and treating soybean root rot, wherein the composition uses chlorogenic acid or at least one of its derivatives as an effective ingredient and is combined with auxiliary materials to form a suitable prevention and treatment agent.

[0010] Furthermore, the composition is in the form of any one of emulsifiable concentrate, suspension concentrate, water dispersible granules, wettable powder and aqueous emulsion.

[0011] It can be seen from the above technical solution that, compared with the prior art, the beneficial effects of the present invention are:

[0012] Chlorogenic acid has a preventive effect on soybean root rot caused by four pathogens, among which the best preventive effect is on root rot caused by Fusarium trilineatum and Fusarium oxysporum, and the preventive effect on root rot caused by Phomopsis spp. and Phytophthora sojae is relatively poor; through the potted plant preventive effect, it can be seen that the treatment of pre-spraying metabolites promotes the growth of soybean seedlings as the concentration increases, and its physiological indicators such as plant height and root length are higher than those of the control treatment without pathogen inoculation, and the disease index is significantly reduced. At 20 days, the preventive effect of 200 μg / mL chlorogenic acid treatment reaches more than 60%, which is slightly higher than the preventive effect of the fungicide mefluoxetine. The present invention shows that chlorogenic acid has excellent preventive potential for soybean root rot, and its development prospects are broad. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0014] Figure 1 The effect of chlorogenic acid on the mycelial growth of five plant pathogenic fungi; Figure 1 A is Phomopsis longicolla; Figure 1 B is Fusarium tricinctum; Figure 1 C is Fusariumoxysporium; Figure 1 D is Phytophthora sojae; Figure 1 E is Rhizopus oryzae;

[0015] Figure 2 This is a potted test of the control effect of chlorogenic acid on four soybean root rot pathogens; Figure 2 A is Fusarium trilineatum; Figure 2 B is Phomopsis; Figure 2 C is Fusarium oxysporum; Figure 2 D is soybean phytophthora and the fungicide is meclodiclofonil. DETAILED DESCRIPTION

[0016] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0017] Example 1

[0018] Determination of the inhibitory effect of chlorogenic acid on five soybean root rot pathogens

[0019] Chlorogenic acid was dissolved in methanol to prepare a 1 mg / mL mother solution. After filtering and sterilizing with a 0.22 μm sterile organic filter membrane, a certain amount of the mother solution was added to the culture medium to make the concentrations reach 50 μl / ml, 100 μl / ml and 200 μl / ml, respectively. A clean water control was set up, and each treatment concentration was repeated 3 times. Fusarium trilineata, Fusarium oxysporum, Phomopsis oryzae and Rhizopus oryzae were cultured on PDA plates, and Phytophthora sojae was cultured on carrot agar medium (CA medium) plates for 5 days and then beaten into fungi (7 mm in diameter) and transferred to the target metabolite culture medium plate. The colony diameter was measured after culturing in a dark environment at 26°C for 5 days, and each treatment was repeated 3 times. The mycelial growth inhibition rate was calculated, and the results are shown in Figure 1 and Table 1.

[0020] Table 1 Effects of chlorogenic acid on the mycelial growth of five plant pathogenic fungi

[0021]

[0022] from Figure 1 As can be seen from Table 1, the inhibition rate of chlorogenic acid at a concentration of 200 μg / mL on the mycelial growth of Phomopsis longicolla, Fusarium tricinctum, F. oxysporium, and Phytophthora sojae, which cause soybean root rot, is above 47%, and it has good biocontrol potential; however, it has no inhibitory effect on the mycelial growth of Rhizopus oryzae, the pumpkin fruit rot pathogen.

[0023] Example 2

[0024] Pot test of the control effect of chlorogenic acid on soybean root rot caused by four soybean root rot pathogens

[0025] On the 2nd and 7th day after inoculation of the pathogen, susceptible soybean varieties were treated with root irrigation with s-chlorofenicol (FS) and different concentrations of chlorogenic acid solution (CK, 50, 100 and 200 μg / mL). After 20 days, the germination rate, plant height, stem diameter, root length and root fresh weight of soybean seedlings were investigated, and the disease index and control effect were calculated. The effects of different concentration treatments were significantly different (P<0.05).

[0026] 1. Effects of chlorogenic acid on Fusarium trilineatum

[0027] Different concentrations of chlorogenic acid treatment had a certain inhibitory effect on soybean seedlings infected by Fusarium trilineatum, and had different degrees of growth-promoting effects on plant height. When the treatment concentration of chlorogenic acid was 100 μg / mL, the germination rate and root length of soybean reached the highest values, which were 95.83% and 13.10 cm, respectively. When the treatment concentration of chlorogenic acid was 200 μg / mL, the plant height, stem diameter and root fresh weight reached the highest values, which were 17.00 cm, 3.13 mm and 2.25 g, respectively. The protection efficiency at this concentration reached 73.89% ( Figure 2 A, Table 2).

[0028] Table 2 Potted plant test of the control effect of chlorogenic acid on soybean Fusarium root rot

[0029]

[0030] Note: The least significant difference (LSD) method was used to compare the treatment groups (P<0.05).

[0031] 2. Effects of chlorogenic acid on Phomopsis

[0032] Different concentrations of chlorogenic acid treatments had an inhibitory effect on the infection of soybean root rot pathogen Phomopsis spp. and promoted the growth of soybean plants. When the treatment concentration was 100 μg / mL, the plant height and root fresh weight reached the highest values, which were 17.17 cm and 2.52 g, respectively. When the treatment concentration of chlorogenic acid was 200 μg / mL, the germination rate, stem diameter and seedling root length reached the highest values, with a germination rate of 87.50%, a stem diameter of 3.13 mm, and a root length of 9.93 cm. The prevention effect reached 70.05% ( Figure 2 B, Table 3).

[0033] Table 3 Potted test of the control effect of chlorogenic acid on soybean Phomopsis root rot

[0034]

[0035] Note: The least significant difference (LSD) method was used to compare the treatment groups (P<0.05).

[0036] 3. Effects of chlorogenic acid on Fusarium oxysporum

[0037] Different concentrations of chlorogenic acid treatment also promoted the growth of soybean seedlings inoculated with Fusarium oxysporum, and the physiological indicators of soybean seedlings increased with the increase of treatment concentration, reaching the highest value at a treatment concentration of 200 μg / mL, with a germination rate of 91.67%, a plant height of 24.67 cm, a stem diameter of 3.17 mm, a root length of 14.30 cm, and a root fresh weight of 1.54 g; at the same time, the protection effect at this concentration reached 66.16% ( Figure 2 C, Table 4).

[0038] Table 4 Potted plant test of the control effect of chlorogenic acid on soybean Fusarium root rot

[0039]

[0040]

[0041] Note: The least significant difference (LSD) method was used to compare the treatment groups (P<0.05).

[0042] 4. Effects of chlorogenic acid on Phytophthora sojae

[0043] The results of antagonistic growth promotion of soybean Phytophthora infection by chlorogenic acid at different concentrations were similar to those of Fusarium oxysporum infection, and both had a growth-promoting effect on soybean seedlings. The growth indicators of soybean increased with the increase of treatment concentration, and reached the highest value when the treatment concentration was 200 μg / mL, with a germination rate of 95.83%, a plant height of 20.83 cm, a stem diameter of 3.17 mm, a root length of 15.27 cm, a root fresh weight of 1.01 g, and a disease index of 12.42. The control efficiency at this concentration reached 66.19% ( Figure 2 D, Table 5).

[0044] Table 5 Potted plant test of the control effect of chlorogenic acid on soybean Phytophthora root rot

[0045]

[0046] Note: The least significant difference (LSD) method was used to compare the treatment groups (P<0.05).

[0047] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. Application of chlorogenic acid in preventing and controlling soybean root rot.

2. The use according to claim 1, characterized in that: The pathogenic bacteria of soybean root rot include: Phomopsis spp., Fusarium trilineata, Fusarium oxysporum, and Phytophthora sojae.

3. A composition for preventing and controlling soybean root rot, characterized in that: The composition uses at least one of chlorogenic acid or its derivatives as an effective ingredient and is prepared with auxiliary materials into a suitable control agent.

4. The composition according to claim 3, characterized in that The composition is present in the form of any one of emulsifiable concentrate, suspension, water-dispersible granules, wettable powder and aqueous emulsion.

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