Traditional Chinese medicine compound microbial fertilizer for inhibiting root rot of angelica sinensis

Abstract

The invention relates to the technical field of fertilizer preparation, in particular to traditional Chinese medicine compound microbial fertilizer for inhibiting angelica sinensis root rot. The compound microbial fertilizer for inhibiting root rot of angelica sinensis comprises 45-55 parts of a compound fertilizer, 20-26 parts of plant ash, 6-10 parts of humic acid, 3-7 parts of diatomite, 4-8 parts of a compound bacterial agent and 8-12 parts of a traditional Chinese medicine extract, the complex microbial inoculant is prepared from bacillus licheniformis, bacillus cereus, bacillus atrophaeus, pseudomonas chlororaphis, pseudomonas brassicae, trichoderma viride and trichoderma harzianum according to the mass ratio of (14 to 18) to (12 to 16) to (11 to 15) to (8 to 12) to (8 to 12) to (5 to 9) to (5 to 9), and the bacterium content is 4 to 6 billion / g; the traditional Chinese medicine extract is prepared from radix scutellariae, herba ephedrae, marigold, oleander, folium ginkgo and radix sophorae flavescentis. Angelica sinensis root rot can be prevented and treated, the yield and quality of angelica sinensis can be remarkably improved, and the effect is excellent.

Description

[0001] This application is a divisional application. The application number of the original application is: 202410774851.5, the application date is 2024.06.17, and the name of the invention is: A composite microbial fertilizer for inhibiting angelica root rot and its preparation method. Technical Field

[0002] The invention relates to the technical field of fertilizer preparation, in particular to a traditional Chinese medicine composite microbial fertilizer for inhibiting angelica root rot. Background Art

[0003] Angelica sinensis (Oliv.) Diels is a perennial herbaceous plant of the Apiaceae family. The root is cylindrical, branched, and yellow-brown; the stem is erect, green-white or purple, with deep grooves; the leaves are pinnate, purple or green, and ovate; the flowers are white, the pedicels are densely covered with fine hairs, the petals are long ovate, and the stylopodium is conical. The flowering period is from June to July. The fruit is elliptical to ovate, with lavender wing edges, and the fruiting period is from July to September. "Compendium of Materia Medica" records: "In ancient times, people married to continue their family line. Angelica regulates blood and is an important medicine for women. It means missing their husbands, so it is named Angelica."

[0004] Nowadays, the demand for angelica is increasing, and the angelica planting area is constantly expanding, resulting in a shortening of the rotation cycle, which has led to a significant increase in angelica diseases, among which root rot is particularly prominent.

[0005] Due to the characteristics of root rot pathogens such as long-term prevalence and rapid onset, root rot is still the main reason for the reduction in yield and quality of angelica. Chemical control can effectively inhibit the growth of pathogens, but excessive use has caused serious harm to the environment and human health. Using green and environmentally friendly plant probiotics to inhibit the growth of root rot pathogens is an important production method for the prevention and control of angelica root rot, and it is also the development trend of the prevention and control of angelica root rot, which can promote the sustainable development of Chinese medicinal materials.

[0006] Therefore, how to provide a microbial preparation for preventing and treating Angelica sinensis root rot is a problem that those skilled in the art need to solve urgently. Summary of the invention

[0007] The invention aims to provide a composite microbial fertilizer for inhibiting angelica root rot and a preparation method thereof.

[0008] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0009] The present invention provides a composite microbial fertilizer for inhibiting root rot of angelica sinensis, which is prepared from the following components by weight: 45-55 parts of composite fertilizer, 20-26 parts of plant ash, 6-10 parts of humic acid, 3-7 parts of diatomaceous earth, 4-8 parts of composite bacterial agent and 8-12 parts of traditional Chinese medicine extract;

[0010] The composite bacterial agent includes: Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas campestris, Trichoderma viride and Trichoderma harzianum;

[0011] The Chinese medicine extracts include: scutellaria baicalensis, ephedra, marigold, oleander, ginkgo leaf and sophora flavescens.

[0012] Preferably, the mass ratio of nitrogen, phosphorus and potassium in the compound fertilizer is 16-22:5-9:11-15.

[0013] Preferably, the mass ratio of Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas campestris, Trichoderma viride and Trichoderma harzianum is 14-18:12-16:11-15:8-12:8-12:5-9:5-9.

[0014] Preferably, the bacterial content of Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas campestris, Trichoderma viride and Trichoderma harzianum is 400-600 million / g.

[0015] Preferably, the mass ratio of scutellaria baicalensis, ephedra, marigold, oleander, ginkgo biloba and sophora flavescens is 10-16:13-17:7-11:8-12:4-8:3-7.

[0016] Preferably, the preparation method of the traditional Chinese medicine extract is: mixing scutellaria baicalensis, ephedra, marigold, oleander, ginkgo leaf and sophora flavescens to obtain a mixed material; mixing the mixed material with water, decocting, and filtering to obtain an extract, which is the traditional Chinese medicine extract.

[0017] Preferably, the mass ratio of the mixture to water is 1:5-7; and the decoction time is 3-5 hours.

[0018] The invention also provides a method for preparing the composite microbial fertilizer, which comprises mixing the composite bacterial agent, wood ash, compound fertilizer, humic acid, diatomaceous earth and traditional Chinese medicine extract.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] Among the components added by the present invention, the use of the composite bacterial agent can effectively prevent and treat the root rot of angelica, Bacillus licheniformis, Bacillus cereus, Pseudomonas campestris and Pseudomonas chlororaphis can effectively prevent and treat Fusarium; Trichoderma viride and Trichoderma harzianum can prevent and treat root-knot nematodes, and Trichoderma viride can also prevent and treat Pseudomonas syringae. Bacillus atrophaeus can prevent and treat Rhizoctonia solani. Therefore, the strains added by the present invention take into account the various pathogenic factors of root rot, have good prevention and treatment effects on various pathogenic bacteria of root rot, and can effectively cope with the influence of complex field environment on the growth of angelica.

[0021] Among the traditional Chinese medicine extracts added by the present invention, scutellaria baicalensis has a significant inhibitory effect on the spore germination of pathogenic bacteria, ephedra has a significant inhibitory effect on the growth of mycelium of pathogenic bacteria, and marigold, oleander, ginkgo leaf and sophora flavescens have excellent effects on killing pathogenic bacteria. The above traditional Chinese medicine components are used in combination, which can effectively prevent and control different development stages of pathogenic bacteria, and can also effectively prevent the infection and spread of root rot pathogens.

[0022] The composite microbial fertilizer provided by the present invention can effectively prevent and treat angelica root rot, and can be applied as base fertilizer during land preparation without topdressing, and can also significantly improve the yield and quality of angelica, with one fertilizer having multiple uses and excellent effects. DETAILED DESCRIPTION

[0023] The invention provides a composite microbial fertilizer for inhibiting root rot of angelica sinensis, which is prepared from the following components in parts by weight: 45-55 parts of compound fertilizer, 20-26 parts of plant ash, 6-10 parts of humic acid, 3-7 parts of diatomaceous earth, 4-8 parts of composite bacterial agent and 8-12 parts of traditional Chinese medicine extract; preferably, 47-53 parts of compound fertilizer, 21-25 parts of plant ash, 7-9 parts of humic acid, 4-6 parts of diatomaceous earth, 5-7 parts of composite bacterial agent and 9-11 parts of traditional Chinese medicine extract; further preferably, 49-51 parts of compound fertilizer, 22-24 parts of plant ash, 8 parts of humic acid, 5 parts of diatomaceous earth, 6 parts of composite bacterial agent and 10 parts of traditional Chinese medicine extract; more preferably, 50 parts of compound fertilizer, 23 parts of plant ash, 8 parts of humic acid, 5 parts of diatomaceous earth, 6 parts of composite bacterial agent and 10 parts of traditional Chinese medicine extract.

[0024] The composite bacterial agent includes: Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas campestris, Trichoderma viride and Trichoderma harzianum;

[0025] The Chinese medicine extracts include: scutellaria baicalensis, ephedra, marigold, oleander, ginkgo leaf and sophora flavescens.

[0026] In the present invention, the mass ratio of nitrogen, phosphorus and potassium in the compound fertilizer is 16-22:5-9:11-15; preferably 17-21:6-8:12-14; more preferably 18-20:7:13; more preferably 19:7:1.

[0027] In the present invention, the mass ratio of Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas campestris, Trichoderma viride and Trichoderma harzianum is 14-18:12-16:11-15:8-12:8-12:5-9:5-9; preferably 15-17:13-15:12-14:9-11:9-11:6-8:6-8; further preferably 16:14:13:10:10:7:7.

[0028] In the present invention, the bacterial content of Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas campestris, Trichoderma viride and Trichoderma harzianum is 400 million to 600 million / g, preferably 500 million / g.

[0029] In the present invention, the mass ratio of scutellaria baicalensis, ephedra, marigold, oleander, ginkgo leaf and sophora flavescens is 10-16:13-17:7-11:8-12:4-8:3-7; preferably 11-15:14-16:8-10:9-11:5-7:4-6; further preferably 12-14:15:9:10:6:5; more preferably 13:15:9:10:6:5.

[0030] In the present invention, the preparation method of the Chinese medicine extract is: mixing scutellaria baicalensis, ephedra, marigold, oleander, ginkgo leaf and sophora flavescens to obtain a mixed material; mixing the mixed material with water for decoction, filtering to obtain an extract, which is the Chinese medicine extract.

[0031] In the present invention, the mass ratio of the mixed material to water is 1:5-7, preferably 1:6.

[0032] In the present invention, the decocting time is 3 to 5 hours, preferably 4 hours.

[0033] The invention also provides a method for preparing the composite microbial fertilizer, which comprises mixing the composite bacterial agent, wood ash, compound fertilizer, humic acid, diatomaceous earth and traditional Chinese medicine extract.

[0034] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0035] Example 1

[0036] A composite microbial fertilizer for inhibiting angelica root rot, the preparation method of which is as follows:

[0037] Mix 45 parts of compound fertilizer, 20 parts of plant ash, 6 parts of humic acid, 3 parts of diatomaceous earth, 4 parts of compound bacterial agent and 8 parts of Chinese herbal medicine extract;

[0038] The composite bacterial agent comprises: Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas rapeseed, Trichoderma viride and Trichoderma harzianum, with a mass ratio of 14:12:11:8:8:5:5; the bacterial content of Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas rapeseed, Trichoderma viride and Trichoderma harzianum is 400 million / g;

[0039] The mass ratio of nitrogen, phosphorus and potassium in the compound fertilizer is 16:5:11;

[0040] The preparation method of the traditional Chinese medicine extract comprises the following steps: mixing scutellaria baicalensis, ephedra, marigold, oleander, ginkgo leaf and sophora flavescens at a mass ratio of 10:13:7:8:4:3 to obtain a mixed material; mixing the mixed material with water at a mass ratio of 1:5 and boiling for 3 hours, and filtering to obtain an extract, namely the traditional Chinese medicine extract.

[0041] Example 2

[0042] A composite microbial fertilizer for inhibiting angelica root rot, the preparation method of which is as follows:

[0043] Mix 55 parts of compound fertilizer, 26 parts of plant ash, 10 parts of humic acid, 7 parts of diatomaceous earth, 8 parts of compound bacterial agent and 12 parts of Chinese herbal medicine extract;

[0044] The composite bacterial agent comprises: Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas rapeseed, Trichoderma viride and Trichoderma harzianum, with a mass ratio of 18:16:15:12:12:9:9; the bacterial content of Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas rapeseed, Trichoderma viride and Trichoderma harzianum is 600 million / g;

[0045] The mass ratio of nitrogen, phosphorus and potassium in the compound fertilizer is 22:9:15;

[0046] The preparation method of the traditional Chinese medicine extract comprises the following steps: mixing scutellaria baicalensis, ephedra, marigold, oleander, ginkgo leaf and sophora flavescens at a mass ratio of 16:17:11:12:8:7 to obtain a mixed material; mixing the mixed material with water at a mass ratio of 1:7 and boiling for 5 hours, and filtering to obtain an extract, namely the traditional Chinese medicine extract.

[0047] Example 3

[0048] A composite microbial fertilizer for inhibiting angelica root rot, the preparation method of which is as follows:

[0049] Mix 50 parts of compound fertilizer, 23 parts of plant ash, 8 parts of humic acid, 5 parts of diatomaceous earth, 6 parts of compound bacterial agent and 10 parts of Chinese herbal medicine extract;

[0050] The composite bacterial agent comprises: Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas rapeseed, Trichoderma viride and Trichoderma harzianum, with a mass ratio of 16:14:13:10:10:7:7; the bacterial content of Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas rapeseed, Trichoderma viride and Trichoderma harzianum is 500 million / g;

[0051] The mass ratio of nitrogen, phosphorus and potassium in the compound fertilizer is 19:7:13;

[0052] The preparation method of the traditional Chinese medicine extract comprises the following steps: mixing scutellaria baicalensis, ephedra, marigold, oleander, ginkgo leaf and sophora flavescens at a mass ratio of 13:15:9:10:6:5 to obtain a mixed material; mixing the mixed material with water at a mass ratio of 1:6 and boiling for 4 hours, and filtering to obtain an extract, namely the traditional Chinese medicine extract.

[0053] Experimental Example 1

[0054] In Anning District, Lanzhou City, a plot of land was selected to plant Angelica sinensis, and the planting variety was Mingui No. 2. Except for the different fertilizer application, the growth management methods of each test group were the same, and each test group only applied basal fertilizer, no topdressing, and used mulch film covering cultivation. The row spacing of Angelica sinensis seedlings was 0.3m×0.4m. Two groups of replicates were set for each test group. The average value of the test results was taken.

[0055] The product obtained by the method of Example 3 was used as the experimental group; the method of use was: 60 kg / mu was applied once as a base fertilizer during land preparation;

[0056] Control group 1, other methods are the same as Example 3, except that Bacillus atrophaeus and Pseudomonas chlororaphis are not added; the use method is: 60 kg / mu is applied as a base fertilizer at one time during land preparation;

[0057] Control group 2, other methods are the same as Example 3, except that no green Trichoderma and harzianum were added; the use method is: 60kg / mu is applied as a base fertilizer once during land preparation;

[0058] Control group 3, other methods are the same as Example 3, the only difference is that Trichoderma viride and Trichoderma harzianum are replaced by equal amounts of Bacillus subtilis and Bacillus megaterium; the use method is: apply 60 kg / mu as a base fertilizer once during land preparation;

[0059] Control group 4, other methods are the same as Example 3, except that no Scutellaria baicalensis and Ephedrae were added; the method of use is: 60 kg / mu is applied as a base fertilizer once during land preparation;

[0060] Control group 5, other methods are the same as Example 3, the only difference is that marigold and oleander are replaced by equal amounts of Bletilla striata and Portulaca oleracea; the method of use is: apply 60 kg / mu as a base fertilizer once during land preparation;

[0061] Prior art group: Referring to the control agent described in Example CN 201710615476.X, root irrigation was performed at the early stage of angelica root rot, 25 kg / mu.

[0062] The incidence rate of Angelica root rot was counted during the peak period of the disease. The yield of Angelica was counted after the Angelica was harvested, and the ferulic acid content and volatile oil content of the Angelica were measured. The results are shown in Tables 1 to 3.

[0063] The incidence rate = (number of diseased plants / total number of plants) × 100%

[0064] The ferulic acid content was determined by high performance liquid chromatography, and the volatile oil determination method was determined according to Method B 2204 of the General Rules of Part IV of the 2020 edition of the Chinese Pharmacopoeia.

[0065] Table 1 Incidence of Angelica root rot

[0066] Incidence % Experimental Group 5.8 Control group 1 9.6 Control group 2 10.4 Control group 3 7.9 Control group 4 10.9 Control group 5 8.7 Existing Technology Group 14.7

[0067] Table 2 Yield of fresh root of Angelica sinensis

[0068]

[0069]

[0070] Table 3 Ferulic acid and volatile oil content

[0071] Ferulic acid % Volatile oil% Experimental Group 0.131 0.534 Control group 1 0.123 0.472 Control group 2 0.125 0.471 Control group 3 0.128 0.510 Control group 4 0.125 0.468 Control group 5 0.127 0.498 Existing Technology Group 0.116 0.423

[0072] It can be seen from the contents recorded in Tables 1 to 3 that the bacterial fertilizer provided by the present invention has excellent technical effects of preventing and treating root rot, and can also significantly improve the yield and quality of angelica sinensis, and its use effect is significantly better than that of existing technical products.

[0073] It can be seen from the data of control groups 1 to 3 that the strains used in the present invention act synergistically with each other. If some of the components are discarded or replaced with other strains with similar effects, the technical effects of the present invention cannot be achieved, which has a significant impact on the fertilizer efficiency of the fertilizer.

[0074] It can be seen from the data of control groups 4 and 5 that the Chinese medicine components used in the present invention also act synergistically with each other. If some of the components are discarded or replaced with other strains with similar efficacy, the technical effect of the present invention cannot be achieved, which has a significant impact on the fertilizer efficiency of the fertilizer.

[0075] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A composite microbial fertilizer for inhibiting root rot of angelica sinensis, characterized in that: The invention is prepared from the following components by weight: 45-55 parts of compound fertilizer, 20-26 parts of plant ash, 6-10 parts of humic acid, 3-7 parts of diatomaceous earth, 4-8 parts of compound bacterial agent and 8-12 parts of Chinese medicine extract; The composite bacterial agent is composed of: Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas campestris, Trichoderma viride and Trichoderma harzianum; The Chinese medicine extract is composed of scutellaria baicalensis, ephedra, marigold, oleander, ginkgo leaf and sophora flavescens; the preparation method of the Chinese medicine extract is: scutellaria baicalensis, ephedra, marigold, oleander, ginkgo leaf and sophora flavescens are mixed to obtain a mixed material; the mixed material is mixed with water and boiled, and the extract obtained by filtering is the Chinese medicine extract; The mass ratio of nitrogen, phosphorus and potassium in the compound fertilizer is 16-22:5-9:11-15; The mass ratio of Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas campestris, Trichoderma viride and Trichoderma harzianum is 14-18:12-16:11-15:8-12:8-12:5-9:5-9; The bacterial contents of Bacillus licheniformis, Bacillus cereus, Bacillus atrophaeus, Pseudomonas chlororaphis, Pseudomonas campestris, Trichoderma viride and Trichoderma harzianum are all 400 million to 600 million per gram.

2. The composite microbial fertilizer according to claim 1, characterized in that: The mass ratio of scutellaria baicalensis, ephedra, marigold, oleander, ginkgo leaf and sophora flavescens is 10-16:13-17:7-11:8-12:4-8:3-7.

3. The composite microbial fertilizer according to claim 1, characterized in that: The mass ratio of the mixed material to water is 1:5-7; and the decoction time is 3-5 hours.

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