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Method for oil-gas resource exploration indicating by utilizing molecular ecology

An ecological and resource technology, applied in the field of microbial oil and gas resource exploration, can solve problems such as lack of comprehensive consideration, and achieve the effect of improving the success rate of exploration and broad prospects for industrial application of exploration

Inactive Publication Date: 2014-10-15
ADVANCED ENERGY & ENVIRONMENTAL TECH INC
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Problems solved by technology

However, this method does not comprehensively consider the changes to the surface environment caused by the micro-leakage of light hydrocarbons reaching the surface, and the impact on the microbial ecology of the surface.

Method used

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  • Method for oil-gas resource exploration indicating by utilizing molecular ecology

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Embodiment 1

[0022] The area involved is a high-altitude permafrost region that includes sites containing well-drilled wells to confirm the existence of gas hydrate deposits. The specific implementation steps of the present invention include:

[0023] 1. The survey line design is carried out in the potential area to be evaluated. The designed survey line passes through the known natural gas hydrate discovery wells and other areas to be evaluated, and the sample collection interval is 250m.

[0024] 2. Make sure that the sampling depth is 20cm, and the sample collection volume is 200g. After the sterilized sample bag is packaged, it is stored in dry ice and frozen in the refrigerator after arriving in the laboratory.

[0025] 3. Under laboratory conditions, take 5 g of the sample and use a DNA extraction kit for soil DNA extraction.

[0026] 4. Use the total bacterial 16S rDNA amplification primer pair 341fGC / 518r with GC clip to carry out PCR amplification on the above extracted DNA, and ...

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Abstract

The invention relates to the field of microorganism oil-gas resource exploration and discloses a method for oil-gas resource exploration indicating by utilizing molecular ecology. The method includes utilizing a molecular ecology studying means to calibrate fingerprint spectra of microorganism species in topsoil or sediment; comparing the fingerprint spectra with references of point locations where underlaying oil-gas reservoirs exist in a known manner to complete dividing of potential abnormality areas and background areas of oil-gas resources. By the method, abnormality dividing of potential oil-gas-resource-containing areas can be realized more accurately, so that exploration success rate is increased.

Description

technical field [0001] The invention relates to the field of microbial oil and gas resource exploration, in particular to a method for instructing oil and gas resource exploration using molecular ecology. Background technique [0002] Oil and gas microbial exploration technology, as a new surface oil and gas exploration method, has been paid more and more attention by the global oil and gas exploration community for its direct, effective, low polysolution and economical advantages. By detecting the changes in the number of hydrocarbon-phagocytic bacteria in the near-surface soil, predicting the distribution of underground oil and gas, and selecting microbial indicators for research, the interference of surface biochemistry can be effectively suppressed, so that microbial abnormalities can more objectively reflect the micro-leakage of oil and gas in the deep underground. Microbial anomalies predict the distribution of subsurface oil and gas. [0003] At present, the microbia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01V9/00
Inventor 邓诗财郝纯张勇梅海李雪
Owner ADVANCED ENERGY & ENVIRONMENTAL TECH INC
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