Mariculture fish multiple anti-idiotypic egg yolk antibody vaccine and preparation method thereof

Abstract

The invention provides a preparation method of a mariculture fish multiple anti-idiotypic egg yolk antibody vaccine. The preparation method comprises the following steps of after inactivating mariculture fish pathogenic bacteria, immunizing mice and rabbits; after using six rabbit anti-pathogenic serums to be mixed with the inactivated mariculture fish pathogenic bacteria, immunizing laying hens;and screening the laying hens, collecting, refining and purifying an obtained coarse anti-idiotypic egg yolk antibody and the mariculture fish multiple anti-idiotypic egg yolk antibody vaccine, wherein the mariculture fish pathogenic bacteria are vibrio anguillarum, Edwardsiella tarda, vibrio alginolyticus, vibrio parahaemolyticus, vibrio vulnificus and streptococcus. The multiple anti-idiotypic egg yolk antibody vaccine provided by the invention can strengthen the resistance of marine fishes to various fish diseases, can prevent various marine fish diseases, can also perform immunization to the marine fishes through an oral way, is simple, convenient and feasible and is liable in obtaining materials at the aspect of preparing an anti-idiotypic egg yolk antibody, is convenient with aseptictreatment, is simple and convenient in preparation programs, low in cost and high in yield and accords with national policy orientation of energy conservation, environmental protection and sustainable development.

Description

Technical field [0001] The invention belongs to the field of aquatic vaccines, and in particular relates to a polyconjugate anti-idiotype egg yolk antibody vaccine for marine cultured fish and a preparation method thereof. Background technique [0002] my country is the country with the largest production of mariculture fish, with 7 billion mariculture fish each year. As the breeding density increases, the water environment continues to deteriorate, and the diseases are becoming more and more serious. The mariculture animals that die from diseases every year cause more than 10 billion economic losses to the country. The main diseases that harm our mariculture animals are bacterial diseases and viral diseases. Bacterial diseases such as Vibrio anguilla, Edwards, Vibrio parahaemolyticus, Vibrio vulnificus, Streptococcus, Vibrio alginolyticus; Viral diseases such as iris virus, shrimp white spot virus and nervous necrosis virus, etc., they cause a large number of marine animals Th...

AI Technical Summary

Problems solved by technology

The main disadvantage of this vaccine is that the inactivation or attenuation method can only eliminate or reduce the growth activity of the pathogen, but cannot eliminate the internal and external toxins contained in the pathogen itself, and improper handling can easily produce toxic side effects

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