Application of bracteose eucalyptus essential oil and complex bacterial agent thereof in preparing insect repellent for marine fishes

A technology of compound bacterial agent and eucalyptus essential oil, which is applied in application, fish farming, climate change adaptation, etc., can solve problems such as damage to the health of aquatic animals, hidden dangers of consumer safety, and unstable drug dosage, so as to reduce bacterial infection and save energy. The cost of breeding, the effect of inhibiting the reproduction of harmful bacteria

Active Publication Date: 2019-12-06
SHANDONG LONGCHANG ANIMAL HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in order to simply pursue immediate results, traditional medicines will cause excessive heavy metals and unstable drug dosages, which will destroy the stability of water quality, damage the health of aquatic animals and reduce their immunity. In severe cases, it will directly lead to death. , ultimately reducing production efficiency and bringing safety hazards to consumers

Method used

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  • Application of bracteose eucalyptus essential oil and complex bacterial agent thereof in preparing insect repellent for marine fishes
  • Application of bracteose eucalyptus essential oil and complex bacterial agent thereof in preparing insect repellent for marine fishes
  • Application of bracteose eucalyptus essential oil and complex bacterial agent thereof in preparing insect repellent for marine fishes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] One, the preparation of Eucalyptus eucalyptus essential oil

[0033] The preparation process of Eucalyptus eucalyptus essential oil in the present invention is as figure 1 As shown, first put the leaves of Eucalyptus eucalyptus in Australia into the washing tower for cleaning, put them in the dryer for drying, and then pulverize them in the pulverizer and then enter the steam distillation kettle, condenser and oil-water stratifier for separation The oil phase and the water phase, the water phase enters the ether extractor to separate the waste water, the liquid containing ether enters the ether recovery tower to recover ether, and the recovered ether and oil phase liquid are added to the solid bed adsorption dryer to further remove the ether in the oil phase Finally, the oil phase liquid is added to a vacuum rectification tower for rectification to remove impurity oils to obtain eucalyptus multibract essential oil.

[0034] The essential oil of Eucalyptus multibract pr...

Embodiment 2

[0044] The eucalyptus eucalyptus essential oil prepared in Example 1 was used for the insect repellent experiment. The test site was selected in Penang, Malaysia, and sea bass were cultured in seawater cages with a size of 0.1 kg. After parasites were detected in the gills of the perch, 1 kg of eucalyptus eucalyptus essential oil was used to mix 1 ton of feed, and the feeding was carried out according to normal feeding and management, and fed continuously for 7 days, 3 times a day. After 7 days, check the gill parasites of the golden perch again. Such as Figure 4 As shown, at the beginning of feeding, the fins of the golden perch were infected with sea lice; after 7 days of feeding, the sea lice on the gills of the golden perch were detected to fall off, which shows that the essential oil of Eucalyptus multibud has an obvious deworming effect on the golden perch.

Embodiment 3

[0046] Golden pomfret was cultured in Zhanjiang cages, and the gills of the fish were found to be red and swollen, with a lot of mucus. Subsequent microscopic examination found that there were many finger ringworms in the gills of the fish. Using 1 kg of eucalyptus eucalyptus essential oil compound microbial agent prepared in Example 1 to mix with 1 ton of feed, carry out according to normal feeding and management, and feed continuously for 7 days, 3 times a day. After 7 days, check the gill parasites of the golden pomfret again. As shown in Figure 5, at the initial stage of feeding, the gills of the golden pomfret were infected with ring worms; after 7 days of feeding, the ring worms in the gills disappeared, which shows that the compound bacterial agent of Eucalyptus eucalyptus essential oil has obvious effect on golden pomfret. Insect repellent effect.

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Abstract

The invention provides application of bracteose eucalyptus essential oil and a complex bacterial agent thereof in preparing an insect repellent for marine fishes. The bracteose eucalyptus essential oil complex bacterial agent comprises the bracteose eucalyptus essential oil and streptomyces griseus HL-50 of which the bacterial content is not smaller than 5*10<8> CFU/g, wherein the preservation number of the streptomyces griseus HL-50 is CGMCC No.18424, and the primary effective constituent of the bracteose eucalyptus essential oil is 1,8-eucalyptol. The bracteose eucalyptus essential oil complex bacterial agent is easy to prepare and use, and the effects of effectively expelling various parasites which infect the marine fishes and inhibiting bacteria can be achieved simply by uniformly mixing the bracteose eucalyptus essential oil complex bacterial agent in edible feed for the marine fishes according to the use amount of 0.5-1.5 kg per ton of the feed and continuously feeding the fishes with the feed for 4-7 days; moreover, the complex bacterial agent is poisonless, harmless, and suitable for being used by the marine fishes for a long term, reduces the breeding cost, and has a wideapplication prospect.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to the application of eucalyptus eucalyptus essential oil and its compound bacterial agent in the preparation of an insect repellant for seawater fish. Background technique [0002] Essential oils are volatile aromatic substances extracted from the flowers, leaves, stems, roots or fruits of plants through steam distillation, extrusion, cold soaking or solvent extraction, most of which have a lighter specific gravity than water. The relative density is generally in the range of 0.8 to 1.17. Essential oils are sensitive to heat and light, so they need to be stored in dark containers and in a cool place, and essential oils are now mostly used in skin care products and aromatherapy because of their special aroma and the characteristics of being soluble in organic solvents, but the function of plant essential oils Various, in addition to the traditional fragrance, soothi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10A23K50/80A23K10/30A23K10/18
CPCA23K10/18A23K10/30A23K50/80A01K61/10Y02A40/81
Inventor 曹爱智籍立民娄倩倩
Owner SHANDONG LONGCHANG ANIMAL HEALTH PROD
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