Method for extracting collagen from sturgeon skin

A technology of collagen and sturgeon skin, applied in animal/human protein, connective tissue peptide, specific peptide, etc., can solve the problems of collagen denaturation and inactivation, and achieve the effect of improving purity, less equipment investment, and simple process

Inactive Publication Date: 2012-07-11
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The current degreasing method of sturgeon skin is mainly organic solvent degreasing method ( Cheng Bo, Wu Jie, Zhang Yurong, et al. Enzymatic extraction of collagen from artificially cultured sturgeon skin [M]. Food Research and Development. 2009.30(3) ), but the organic solvent denatures the collagen, leading to its inactivation; the method of removing non-collagen mainly includes soaking in 0.1M NaOH ( Lingzhao Wang, Bao Yang , Xiuqiao Du, et al., Optimization of conditions for extraction of acid-soluble collagen from grass carp(Ctenopharyngodon idella) by response surface methodology[J]. Innovative Food Science and Emerging Technologies.2008.9 ), but NaOH is a strong base, which can easily denature collagen and lead to its inactivation; the extraction process of collagen is ma

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Pretreatment of sturgeon skin: Take out the frozen sturgeon skin, thaw it, remove the subcutaneous meat, fat and connective tissue, cut it into pieces of fish skin about 0.5cm×0.5cm in size, add 0.5℅(m / V) sodium carbonate Soak to remove fat until the fat is completely removed, wash; then soak in 3.5℅ (m / V) sodium chloride solution for 24 hours to remove soluble non-collagen protein, wash, and dry for later use.

[0015] Mix 5g of pretreated sturgeon skin with distilled water at a ratio of 1:5 (m / m), stir evenly, add 1℅ (enzyme / substrate, m / m) trypsin, at the optimum temperature of trypsin Stir at 37°C and pH 7, extract for 6 hours, filter, collect the filtrate and add ammonium sulfate for salting out, centrifuge at 4000 r / min at room temperature for 20 min, discard the supernatant, dissolve the precipitate with 0.5 mol / L acetic acid, and dialyze Dialyze the distilled water in the bag for 24 hours, change the distilled water every 2 hours, and freeze-dry to obtain collag...

Embodiment 2

[0017] Pretreatment of sturgeon skin: Take out the frozen sturgeon skin, thaw it, remove the subcutaneous meat, fat and connective tissue, cut it into pieces of fish skin about 0.5cm×0.5cm in size, add 0.5℅(m / V) sodium carbonate Soak to remove fat until the fat is completely removed, wash; then soak in 3.5℅ (m / V) sodium chloride solution for 24 hours to remove soluble non-collagen protein, wash, and dry for later use.

[0018] Mix 5g of pretreated sturgeon skin with distilled water at a ratio of 1:10 (m / m), stir evenly, add 2℅ (enzyme / substrate, m / m) trypsin, at the optimum temperature of trypsin Stir at 37°C and pH 7, extract for 9 hours, filter, collect the filtrate and add ammonium sulfate for salting out, centrifuge at 4000 r / min at room temperature for 20 min, discard the supernatant, dissolve the precipitate with 0.5 mol / L acetic acid, and dialyze Dialyze the distilled water in the bag for 24 hours, change the distilled water every 2 hours, and freeze-dry to obtain col...

Embodiment 3

[0020] Pretreatment of sturgeon skin: Take out the frozen sturgeon skin, thaw it, remove the subcutaneous meat, fat and connective tissue, cut it into pieces of fish skin about 0.5cm×0.5cm in size, add 0.5℅(m / V) sodium carbonate Soak to remove fat until the fat is completely removed, wash; then soak in 3.5℅ (m / V) sodium chloride solution for 24 hours to remove soluble non-collagen protein, wash, and dry for later use.

[0021] Mix 5g of pretreated sturgeon skin with distilled water at a ratio of 1:15 (m / m), stir evenly, add 3℅ (enzyme / substrate, m / m) trypsin, at the optimum temperature of trypsin Stir at 37°C and pH 7, extract for 12 hours, filter, collect the filtrate and add ammonium sulfate for salting out, centrifuge at 4000 r / min at room temperature for 20 min, discard the supernatant, dissolve the precipitate with 0.5 mol / L acetic acid, and dialyze Dialyze the distilled water in the bag for 24 hours, change the distilled water every 2 hours, and freeze-dry to obtain coll...

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Abstract

The invention relates to a method for extracting collagen from sturgeon skin, belongs to the technical field of food waste recycling. The method is characterized in that lipide is removed with diluted polybasic weak base, and non-collagen is removed by soaking with 3.5% sodium chloride solution for 24h, so that the collagen activity is not influenced; by adopting pancreatin to extract, the extracting is more sufficient, a best extracting effect is obtained, the collagen purity is improved, and the denaturalization and inactivation of the collagen are not caused due to mild extracting conditions; and no toxic chemicals are added, harmful substance residue is not existed, and no pollution is caused to the collagen and environment, thus the treatment technique is environment-friendly and green. Therefore, the method is simple in process, low in equipment investment and economical, and is suitable for industrial production.

Description

technical field [0001] The invention relates to a method for extracting collagen from sturgeon skin, which belongs to the technical field of waste recycling in food. Background technique [0002] The Latin name for the sturgeon is Acipenser sturio Linnaeus, The English name is Common sturgeon, which belongs to the phylum Chordate, Osteophyta, Radininis, Phylophora, Sturgeon, Acipensidae, Acipenserus. Sturgeon is an existing ancient biological population, which originated in the Cretaceous period hundreds of millions of years ago, and is known as the "panda" in the water ( Tang Zong.Nutrition and processing of sturgeon[J].Beijing Aquatic Products.2002:26-27 ). Sturgeon is the oldest fish in the world with the largest body size, the longest lifespan. At present, there are still 26 species of sturgeon (Huso) on the earth, which are concentrated in the northern hemisphere. There are 8 species in the rivers and coastal areas of our country, of which 3 species are distributed ...

Claims

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Application Information

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IPC IPC(8): C07K14/78C07K1/14
Inventor 仰榴青冯伟伟张敏吴向阳赵江丽赵婷李芳邹烨
Owner JIANGSU UNIV
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