Method for extracting collagen from sturgeon skin

Abstract

The invention relates to a method for extracting collagen from sturgeon skin, belongs to the technical field of food waste recycling. The method is characterized in that lipide is removed with diluted polybasic weak base, and non-collagen is removed by soaking with 3.5% sodium chloride solution for 24h, so that the collagen activity is not influenced; by adopting pancreatin to extract, the extracting is more sufficient, a best extracting effect is obtained, the collagen purity is improved, and the denaturalization and inactivation of the collagen are not caused due to mild extracting conditions; and no toxic chemicals are added, harmful substance residue is not existed, and no pollution is caused to the collagen and environment, thus the treatment technique is environment-friendly and green. Therefore, the method is simple in process, low in equipment investment and economical, and is suitable for industrial production.

Description

technical field [0001] The invention relates to a method for extracting collagen from sturgeon skin, which belongs to the technical field of waste recycling in food. Background technique [0002] The Latin name for the sturgeon is Acipenser sturio Linnaeus, The English name is Common sturgeon, which belongs to the phylum Chordate, Osteophyta, Radininis, Phylophora, Sturgeon, Acipensidae, Acipenserus. Sturgeon is an existing ancient biological population, which originated in the Cretaceous period hundreds of millions of years ago, and is known as the "panda" in the water ( Tang Zong.Nutrition and processing of sturgeon[J].Beijing Aquatic Products.2002:26-27 ). Sturgeon is the oldest fish in the world with the largest body size, the longest lifespan. At present, there are still 26 species of sturgeon (Huso) on the earth, which are concentrated in the northern hemisphere. There are 8 species in the rivers and coastal areas of our country, of which 3 species are distributed ...

AI Technical Summary

Problems solved by technology

[0006] The current degreasing method of sturgeon skin is mainly organic solvent degreasing method ( Cheng Bo, Wu Jie, Zhang Yurong, et al. Enzymatic extraction of collagen from artificially cultured sturgeon skin [M]. Food Research and Development. 2009.30(3) ), but the organic solvent denatures the collagen, leading to its inactivation; the method of removing non-collagen mainly includes soaking in 0.1M NaOH ( Lingzhao Wang, Bao Yang , Xiuqiao Du, et al., Optimization of conditions for extraction of acid-soluble collagen from grass carp(Ctenopharyngodon idella) by response surface methodology[J]. Innovative Food Science and Emerging Technologies.2008.9 ), but NaOH is a strong base, which can easily denature collagen and lead to its inactivation; the extraction process of collagen is mainly acid extraction ( Xiao Gao, Shi Yidong, Chen Yanxia. Extraction of Collagen and Its Modification of Textile Materials[M]. Research and Technology.2010.2 ) and pepsin extraction ( Phanat Kittiphattanabawon , Soottawat Benjakul , Wonnop Visessanguan, et al. Isolation and characterization of collagen from the cartilages of brownbanded bamboo shark (Chiloscyllium punctatum) and blacktip shark (Carcharhinus limbatus[J]). Peng Dexiang; Yan Rujuan et al. Collagen Active Peptide and Its Preparation and Application [P], Chinese Patent, 200810037217 ), the pH value of this kind of extraction method is low, generally 2-3, and it is also easy to denature and inactivate the protein (for example, the optimum pH of pepsin is 2.0, and the collagen has been denatured and inactivated at this time)