Collagen peptide with light aging relieving effect and preparation method thereof

A collagen peptide and photoaging technology, applied in the biological field, can solve the problems of unsatisfactory production needs, collagen peptide safety and biological activity to be verified, and achieve large commercial production value, significant anti-oxidation effect, and improved damage. Effect

Inactive Publication Date: 2020-01-21
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A single collagen extraction method cannot meet the production needs, and the safety and biological activity of the obtained collagen peptides need to be verified

Method used

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  • Collagen peptide with light aging relieving effect and preparation method thereof
  • Collagen peptide with light aging relieving effect and preparation method thereof
  • Collagen peptide with light aging relieving effect and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A collagen peptide with a photoaging-improving effect, the preparation method of which comprises the following steps:

[0037] (1) Raw material processing: scrape off the remaining fish meat on the eel skin, cut into about 1cm in size 2 Add the fragments into ice water, stir and wash repeatedly with a magnetic stirrer, and replace the ice water until the solution becomes clear;

[0038] (2) Removal of non-collagen: Soak the minced eel skin in 0.1mol / L NaOH solution for 48 hours according to the ratio of material to liquid 1:10 g / mL, and replace the NaOH solution every 12 hours during the soaking process (in the first After soaking the eel skin to swell, chop the eel skin again to improve the soaking effect); then wash the eel skin with distilled water until the cleaning solution is neutral;

[0039] (3) Degreasing: Soak the eel skin again with a butanol solution with a volume concentration of 10% according to the ratio of material to liquid 1:10 g / mL for 48 hours, and ...

Embodiment 2

[0047] In step (4), the soaking time in acetic acid was changed to 18 h, and the rest of the operations were the same as in Example 1.

Embodiment 3

[0049] In step (4), the soaking time in acetic acid was changed to 30 h, and the rest of the operations were the same as in Example 1.

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Abstract

The invention discloses a collagen peptide with a light aging relieving effect and a preparation method thereof. The preparation method is characterized in that eel skins are cut up and cleaned, and are then sequentially soaked by a NaOH solution and a butanol solution to remove noncollagen protein and fat; then, an acid method and an enzyme method are used for coextraction to obtain collagen peptide; finally, the collagen peptide is digested through in vitro simulation; and collagen peptide powder is obtained. The method of compounding the acid method and the enzyme method is used for extracting collagen peptide in the eel skins; the extraction rate reaches 80 percent or higher; the purity reaches 85 percent or higher; the collagen peptide obtained through final hydrolysis can relieve symptoms such as skin collagen peptide loss caused by light aging; and good effects of beautification, skin protection and senescence delaying are achieved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a fish skin collagen peptide with photoaging-improving effect and a preparation method thereof. Background technique [0002] Photoaging is a series of functional degenerative changes caused by repeated exposure to ultraviolet rays. The impact of photoaging on the skin is mainly manifested in skin relaxation, dark yellow, wrinkles, pigmentation, etc., and severe cases can even lead to malignant tumors of the skin. There are many biologically active substances that can resist UV damage in nature, such as vitamins and their derivatives, antioxidant enzymes, plant extracts such as aloe vera, oat and grape seed extract, etc., which mainly remove or reduce free radicals and their intermediate products, Block or slow tissue damage. Therefore, the intake of biologically active substances can physiologically prevent skin aging without causing damage to the body. [0003] As a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C12P21/06C07K1/14C07K1/34C07K1/36
CPCC07K14/78C12P21/06
Inventor 张宁宁郑雨婷毛极仁王睿陈静
Owner FUJIAN AGRI & FORESTRY UNIV
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