High-purity fishy smell and foreign odor-free fish collagen protein peptide and preparation method thereof

Abstract

The invention discloses a high-purity fishy smell and foreign odor-free fish collagen protein peptide and a preparation method thereof. The preparation method comprises the following steps: cleaning fish skins, and then cutting into blocks and mincing; performing stirring treatment with NaCl solution; centrifugally degreasing to remove paraprotein; adding water to regulate the pH value of initial slurry to be 8.0-8.5; performing combined gradient enzymolysis on alkali protease and neutral protease; deactivating enzyme after the enzymolysis is ended; performing adsorption bleaching by activated carbon; and then performing rough filtration, fine filtration, evaporation concentration and spray drying to prepare the high-purity fishy smell and foreign odor-free fish collagen protein peptide. The high-purity fishy smell and foreign odor-free fish collagen protein peptide disclosed by the invention has a simple process and is easy to industrial production; fishy smell, foreign odor and non-collagen proteins are fully removed in production; the low-temperature biological enzymolysis technology is adopted, and other substances are not added, thereby, the product quality is improved; an obtained fish collagen protein peptide powder has the characteristics the content of amino acid hydroxyproline is not less than 9 percent, and the average molecular weight is lower than 1000Dal; no fishy smell or foreign odor cannot generated, and obvious fishy smell or foreign odor also cannot be generated through heating treatment under the acid conditions. The high-purity fishy smell and foreign odor-free fish collagen protein peptide can be widely applied to processing of various foods and cosmetics as a green functional ingredient.

Description

technical field [0001] The invention relates to the technical field of high value-added processing and utilization of aquatic product waste, in particular to the preparation of high-purity fish collagen peptide without fishy smell by pretreatment of tilapia processing waste-fish skin, compound enzyme gradient enzymolysis and refining , the peptide will not produce fishy smell when heated under acidic conditions, and can be widely used as raw materials for health food, general food and cosmetics. Background technique [0002] Collagen is the most important tissue component of human skin, bones, and connective tissue. It has been scientifically proven that adults naturally lose about 3-5 grams of collagen every day. If it is not replenished in time, on the one hand, tissue cells will not be able to obtain raw materials for protein synthesis; on the other hand, nutrients such as vitamins and trace elements will not easily reach the corresponding cells and organs due to the lac...

AI Technical Summary

Problems solved by technology

[0004] The existing fish collagen peptide processing methods mainly have the following problems: (1) The traditional collagen peptide extraction method uses acid or alkali to degrade collagen for extraction. These methods will cause some amino acids to be destroyed during the degradation process, and the product safety is poor. Loss of biological activity; (2) Enzyme hydrolysis technology is used to prepare fish collagen peptides. The combination of enzymes is not ideal, the production efficiency is low, and the product molecular weight is high. During the enzymolysis process, a large amount of industrial grade hydrochloric acid and sodium hydroxide are added to adjust the pH value. Affect product quality, and increase product refining difficulty and production cost; (3) Due to untimely raw material treatment or production process to remove fishy smell and odor, the developed fish collagen product has a strong fishy smell, which limits its use in downstream products or the deodorization and deodorization refining process is very complicated, such as Chinese patent 200810138406.0 discloses a fish collagen peptide ion exchange chromatography deodorization process, which uses fish collagen peptide pretreatment, transformation, and carboxymethyl cellulose exchange column, pure water elution, desalination, and then concentrated and dried into peptide powder that removes fishy smell; Chinese patent 200810106710.7 discloses a method for processing fish collagen oligopeptides using deashed and degreased dried fish scales as raw materials, and its refining uses silicon Algal earth filtration, macroporous adsorption resin adsorption, cation exchange resin adsorption, anion exchange resin adsorption, eluent elution, multi-layer filtration membrane filtration method; The industrial production method of fish collagen peptide, its refining includes centrifugation to remove slag, oil-water centrifuge deoiling and deodorization, microfiltration and ultrafiltration clarification, 5% powder activated carbon decolorization, and filtration process; these methods are complex in process and cumbersome in operation. Not conducive to industrial production

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