Mimic polypeptide of non-collagen protein and application of the mimic peptide in biomimetic mineralization

A collagen and polypeptide technology, applied in the directions of containing abnormal peptide bonds, compression mold cups, chemical instruments and methods, etc., can solve the problem of limited binding ability of hydroxyapatite and collagen substrates, and achieve the promotion of mineral nucleation and growth. , to promote repair, promote the effect of nucleation and growth

Inactive Publication Date: 2011-11-16
TSINGHUA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The latter, as a biomimetic repair material, has a composition and structure similar to that of human bone, but the ability of hydroxyapatite to bind to collagen substrates is limited, so compared with natural human bone and dentin, its mechanical properties need to be further improved

Method used

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  • Mimic polypeptide of non-collagen protein and application of the mimic peptide in biomimetic mineralization
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  • Mimic polypeptide of non-collagen protein and application of the mimic peptide in biomimetic mineralization

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Embodiment 1

[0035] Embodiment 1, the application of polypeptide

[0036] 1. Polypeptide nucleation and mineralization experiments

[0037] The mineralization ability of the peptide was analyzed using a steady-state agarose gel system. The schematic diagram of the steady-state gel reaction system is shown in figure 1 shown. An appropriate amount of agarose (agarose) was weighed and heated in deionized water until boiling to dissolve, and configured as a 3% agarose solution. Cool to below 50°C, add NaCl, Tris-HCl and polypeptide mother solution respectively, to the final concentration: 2% agarose, 150mM NaCl, 10mM Tris-HCl, the peptide concentrations used in different experimental groups were 0, 0.03, 0.3, 3, 30 and 100 μg / ml. 5 ml of the prepared agarose mixed solution was quickly loaded into a reaction tank sealed with a semi-permeable membrane (molecular weight cut-off: 1000 Da). After it is gelled, the calcium and phosphorus reaction solutions are introduced. Calcium reaction solu...

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Abstract

The invention discloses mimic polypeptide of non-collagen protein and application of the mimic polypeptide in biomimetic mineralization. An amino acid sequence of the polypeptide in the invention is represented as SEQ ID NO:1. Function and effect of polypeptide mimic soluble non-collagen protein (BSP and DMP1) in the invention are as follows: controlling deposition and growth of mineral in I type collagen, further simulating formation processes of human body natural hard tissues, promoting mineral nucleation and growth, increasing combination capability of the mineral and collagen fiber, raising mineralization degree of the mineral especially hydroxy apatite in the I type collagen, thereby promoting restoration of bone and dentine.

Description

technical field [0001] The invention relates to a non-collagen mimic polypeptide and its application in biomimetic mineralization. Background technique [0002] Hard tissues such as bone and dentin are mainly composed of ordered and multi-level directional arrangements of mineralized collagen fibers. Type I collagen template protein is the main matrix protein of bone and dentin, and hydroxyapatite is deposited on the surface and interstitial regions of type I matrix protein. These minerals, especially the hydroxyapatite mineral in the interstitial zone, play a key role in the excellent mechanical properties of bone and dentin. [0003] There are currently two main types of repair materials for bone and dentin. One is a cement (bone cement, etc.) mainly composed of inorganic minerals or macromolecules to fill the defect tissue; the other is a hard tissue bionic repair material blended with hydroxyapatite and collagen. After all, the former is different from human bones. Af...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/02A61K6/02A61L27/22A61L27/46
Inventor 王秀梅王琼程振江崔福斋
Owner TSINGHUA UNIV
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