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Hernia bioremediation mesh and preparation method thereof

A biological and mesh technology, applied in medical science, prosthesis, etc., can solve the problems of tissue regeneration mismatch, cytotoxicity, slow degradation rate, etc., to reduce the incidence of inflammation and infection, accelerate functional reconstruction, good The effect of mechanical properties

Inactive Publication Date: 2019-01-22
上海白衣缘生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] In practical applications, the acellular material patch needs to introduce chemical cross-linking agents such as epoxy or glutaraldehyde during its processing due to its insufficient mechanical strength, which has the following disadvantages: potential cytotoxicity and slow degradation rate , does not match tissue regeneration, which can lead to fibrosis, chronic inflammation, etc.
Two patents on hernia mesh with publication numbers CN108261565 and CN105999411 have optimized the decellularization process, but in order to meet the mechanical requirements of hernia repair surgery, it is inevitable to add a cross-linking process

Method used

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  • Hernia bioremediation mesh and preparation method thereof
  • Hernia bioremediation mesh and preparation method thereof
  • Hernia bioremediation mesh and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Preparation of biorepair mesh for submucosal hernia of pig small intestine, please refer to Figure 5 :

[0056] (1) Pretreatment

[0057] Take freshly slaughtered pig small intestine tissue and clean it, soak it in 0.5% acetic acid solution for 30 minutes, the ratio of pig small intestine to acetic acid solution is 1:5, use physical curettage to remove the mucosal layer, muscle layer, serous layer of the pig small intestine jejunum , Lymph nodes, separate the submucosa, cut into thin strips evenly longitudinally, and rinse with purified water 3 times to obtain the biorepair material, namely the submucosa of the small intestine, which is referred to as SIS material below.

[0058] (2) Virus inactivation

[0059] Using a mixed aqueous solution containing 1.0% peroxyacetic acid and 15% ethanol, the ratio of the SIS material to the mixed aqueous solution is 1:10, and the virus is inactivated by soaking at room temperature for 100 minutes under ultrasonic conditions. Then use pur...

Embodiment 2

[0070] Preparation of pig small intestinal submucosal hernia repairing mesh

[0071] (1) Pretreatment

[0072] Clean the small intestine tissue of the freshly slaughtered animal and soak it in 0.01% acetic acid solution for 120 minutes. The ratio of the small intestine to the acetic acid solution is 1:10. Use physical scraping to remove the mucosal, muscle, and serous layers of the small intestine and jejunum. , Lymph nodes, separate the submucosa, cut into fragments, and rinse 3 times with purified water.

[0073] (2) Virus inactivation

[0074] Using a mixed aqueous solution containing 0.5% peroxyacetic acid and 25% ethanol, the ratio of the SIS material to the mixed aqueous solution is 1:15, and the virus is inactivated by soaking at room temperature for 120 minutes under ultrasonic conditions. Then use purified water ultrasonic cleaning 3 times.

[0075] (3) Degreasing

[0076] Use ethanol with a concentration of 90%, the ratio of SIS material to ethanol is 1:15, and soak at room t...

Embodiment 3

[0085] For the safety of the samples, the samples prepared in Example 1-2 were tested for immunogenic substances.

[0086] (1) Cell residue detection method: fixed with 10% neutral formalin, embedded in paraffin, cut into 0.4 micron slices, dewaxed with xylene, dehydrated with serial alcohol, stained with hematoxylin and eosin, microscope Observe the cell residue and matrix fiber structure.

[0087] (2) DNA content detection method: in accordance with YY / T 0606.25-2014 "Determination of DNA Residues of Biological Materials of Animal Origin: Fluorescence Staining Method".

[0088] (3) Detection method of α-Gal antigen content: After the sample is fixed with paraformaldehyde, the slice is embedded in conventional paraffin, and the slice thickness is 3 microns. The specific affinity between biotin-labeled BSI-B4 and α-Gal antigen was used for immunohistochemical reaction. Staining result judgment: dark brown particles are strongly positive (+++), brown particles are positive (++), yel...

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Abstract

The invention discloses a hernia bioremediation mesh and a preparation method thereof. The hernia bioremediation mesh is made of animal tissues, immune ingredients such as cells and DNA are removed, and the animal tissues are cut into thin strips, twisted into threads, and woven into the bioremediation mesh. Xenogeneic decellularized materials obtain greater intensity through twisting into the threads, so that the xenogeneic decellularized materials are not prone to breaking; since the threads are interwoven with each other after weaving, external force of tensile tearing does not act on one point but on a plurality of interwoven points, so that the mechanical properties are good; and the defects that the xenogeneic decellularized material hernia bioremediation mesh has poor mechanical intensity and needs to be introduced with cross-linking agents or synthetic materials are overcome. The structure of the mesh is prepared by a weaving process, and the size of a mesh aperture can be easily adjusted through weaving parameters, so that requirements of practical application are met. Meanwhile, the hernia bioremediation mesh retains an original three-dimensional structure, non-collagen proteins, growth factors and other components of an extracellular matrix, so that the functional reconstruction and postoperative healing of the tissues are promoted.

Description

Technical field [0001] The invention relates to the field of biological materials, in particular to a hernia biological repair mesh and a preparation method thereof. Background technique [0002] Hernia refers to any organ or tissue that has left its original position and enters another part through a normal or abnormal congenital or acquired weakness, defect or pore in the human body. Surgery is currently the only effective treatment. Tension-free hernia repair is currently the main surgical method for hernia. With the rapid development of medical biomaterials, various hernia repair materials have been widely used in clinical practice. [0003] Hernia patches are mainly divided into two categories: synthetic materials and biological materials. [0004] ①Synthetic material patch [0005] The main component of the hernia patch made of synthetic material is multi-polymer, including two types: degradable and non-degradable. The non-degradable synthetic material patch has good tensile...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/58
CPCA61L27/3633A61L27/3687A61L27/3691A61L27/58A61L2300/252A61L2300/404A61L2300/41
Inventor 韩韦红葛翠兰钱锵周秀清
Owner 上海白衣缘生物工程有限公司
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