Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bonding-assisting biological mineralization material and applications thereof in biomimetic mineralization

A biomineralization and bonding technology, applied in the field of biomimetic biomaterial engineering, can solve the problems of short action time and poor mineralization effect, and achieve the effects of convenient storage, good bonding strength, and simple and effective application.

Inactive Publication Date: 2017-03-08
ZHEJIANG UNIV
View PDF3 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Utilize the adhesive auxiliary mineralization material of the present invention, mix with the adhesive, after curing, it can adhere to the tooth surface stably for a long time, continuously and stably provide the source of calcium and phosphorus, and solve the problem of short action time of the current remineralization preparations, mineralization The problem of poor remineralization effect, promotes the remineralization of type I collagen and its demineralized enamel and dentin

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bonding-assisting biological mineralization material and applications thereof in biomimetic mineralization
  • Bonding-assisting biological mineralization material and applications thereof in biomimetic mineralization
  • Bonding-assisting biological mineralization material and applications thereof in biomimetic mineralization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1.1 Synthesis of nACP particles (PAA-ACP) stabilized with non-collagen analog polyacrylic acid (PAA)

[0034] Using the solution precipitation method, drop an equal volume of calcium chloride solution into the mixed solution of sodium dihydrogen phosphate and PAA. During the titration process, the pH value of the solution is controlled at 9.5±0.5, and the titration time is controlled at 0.5-1h. The final molar concentration ratio of calcium and phosphorus is 10:6, and the final concentration of PAA is 500ug / ml. Centrifuge and place in a vacuum oven overnight. The next day, the synthesized nACP particles were stored in polyethylene plastic bottles sealed at room temperature. Transmission electron microscope (TEM) observation observes the regularity of ACP particle morphology that PAA is stable, and size is about 20-100nm (attachment figure 1 ). The ACP synthesized by Fourier transform infrared analysis (FTIR) is at 580cm -1 There is a peak at the place, no cleavage o...

Embodiment 2

[0040] 2.1 Synthesis of nACP particles (PAsp-ACP) stabilized with non-collagen analog polyaspartic acid (PAsp)

[0041] Using the solution precipitation method, mix the mixture of sodium dihydrogen phosphate and PAsp with an equal volume of calcium chloride solution for 10-30 minutes, the final concentration ratio of calcium and phosphorus is 10:6, and the final concentration of Pasp is 240ug / ml. Centrifuge and place in a vacuum oven overnight. The next day, the synthesized solid nACP particles were stored in polyethylene plastic bottles sealed at room temperature. Observation and observation of ACP morphological rules with a transmission electron microscope (TEM), the size is about 20-80nm (attached Figure 6 ). The synthesized ACP detected by XRD is an amorphous phase (attached Figure 7 ).

[0042] 2.2 Preparation of recombinant type I collagen model, simulating dentin type I collagen: same as in Example 1

[0043] 2.3 Mineralization of monolayer recombinant type I col...

Embodiment 3

[0046] 3.1 Synthesis of nACP particles (PAA-ACP) stabilized with non-collagen analog polyacrylic acid (PAA) was the same as in Example 1, and the final concentration of PAA was 300ug / ml.

[0047] 3.2 Preparation of recombinant type I collagen model to simulate dentin type I collagen

[0048] Same as Example 1

[0049] 3.3 Mineralization of monolayer recombinant type I collagen

[0050] According to 5wt% of the dosage of the adhesive, the above-mentioned synthetic solid PAA-ACP particles are mixed with a commercial adhesive such as Clearfil S3 Bond adhesive, stirred evenly, and brushed on the TEM nickel mesh loaded with type I recombinant collagen , stand still for 30-60s, and light cure for 40s. Suspend the nickel mesh in the artificial saliva. After 21 days, TEM and SAED observed that the mineralization of the collagen appeared in the fiber, and the TEM picture was the same as that in Example 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a bonding-assisting biological material and applications of the bonding-assisting biological material in biomimetic mineralization, for promoting the remineralization of type I collagen, demineralized enamel and dentin. The bonding-assisting biological mineralization material is the synthetized amorphous calcium phosphate particles stabilized by non-collagenous protein analogue. According to the applications of the bonding-assisting biological mineralization material in biomimetic mineralization, the assisting mineralization material is mixed with a self-etching adhesive, thus the bonding-assisting biological mineralizer is prepared, the bonding-assisting biological mineralizer coats the surfaces of single-layer recombinant type I collagen, the demineralized enamel and the dentin, and then the mineralization property of the biological mineralizer for the single-layer recombinant type I collagen, the demineralized enamel and the dentin is studied. The existing biological mineralization mode (solutions and paste are adopted, namely, gargling or local coating is adopted) is changed, the assisting mineralization material is mixed with the adhesive, after curing, the liquid state is converted into the solid state, then the adhesive anti-caries coating is formed, and the adhesive anti-caries coating can be adhered to the part needing mineralization for a long term, so that the acting time with teeth is prolonged, the use is simple and convenient, and the self-repairing of the demineralized teeth is promoted.

Description

technical field [0001] The invention belongs to the technical field of biomimetic biomaterial engineering, and in particular relates to an adhesive-assisted biomineralization material and its application in biomimetic mineralization to promote type I collagen and remineralization of demineralized tooth enamel and dentin . Background technique [0002] Amorphous mineral phases, including silica, amorphous calcium phosphate and amorphous calcium carbonate, exist widely in nature and are the first mineral phases formed during biomineralization. The formation of dental hard tissue is actually a biomineralization process, in which the nanoparticle precursor phase, that is, amorphous calcium phosphate (amorphous calcium phosphate, ACP), plays a key role. Hydroxyapatite (Hydroxyapatite, HAP) constitutes the inorganic main part of teeth, endowing teeth with good mechanical hardness and biological functions. In the process of biomineralization, the core is the orderly deposition an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K6/033A61K6/083A61K6/838
CPCA61K6/838A61K6/889
Inventor 傅柏平吴志芳金晓婷王喆洪艳王晓侃
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com