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Preparation method and application of tuna skin high-moisturizing bioactive peptide

A technology of tuna fish and active peptides, which is applied in the direction of peptide preparation methods, skin care preparations, chemical instruments and methods, etc., can solve the problems of not conforming to the green sustainable economic development model, high temperature requirements, high energy consumption, etc., and achieve The process is easy to control, the preparation method is simple, and the skin moisturizing effect is good

Inactive Publication Date: 2016-12-07
WUHAN BEST CARRIER NANO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Acid method, alkali method, salt method, and hot water extraction method are high-energy-consuming industries, and the acid-base salt discharged is easy to pollute the surrounding environment, which does not conform to the green sustainable economic development model
The biological enzyme method is to use the properties of enzyme temperature reaction and high efficiency to extract collagen from different raw materials; however, the biological enzyme method has the disadvantages of high temperature requirements and poor reaction stability.

Method used

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  • Preparation method and application of tuna skin high-moisturizing bioactive peptide
  • Preparation method and application of tuna skin high-moisturizing bioactive peptide

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preparation example Construction

[0025] The invention provides a preparation method of high moisturizing active peptide of tuna skin, comprising the following steps:

[0026] 1) Preliminarily pulverizing tuna skin to obtain tuna skin slurry;

[0027] 2) pulverizing the tuna skin slurry obtained in the step 1) to obtain a fine tuna skin slurry;

[0028] 3) mixing the fine tuna skin slurry obtained in step 2) with water and then performing solid-liquid separation to remove non-collagenous water-soluble substances to obtain tuna skin precipitation;

[0029] 4) carrying out the enzymolysis reaction with the tuna skin precipitation and enzyme obtained in the step 3) in the water system to obtain the tuna skin enzymatic hydrolysis solution;

[0030] 5) Using an ultrafiltration membrane to separate the step 4) to obtain the enzymatic hydrolyzate, and separate to obtain an active polypeptide with a molecular weight of less than 2KD. The active polypeptide with a molecular weight of less than 2KD is a highly moisturi...

Embodiment 1

[0045] Soak tuna skin and water 1:4 and wash for 15 minutes, set aside;

[0046] Put the soaked and cleaned tuna skin into a pulverizer and process it into a slurry, and set it aside;

[0047] Enter the slurry into a colloid mill for homogenization treatment to obtain a material body with a particle size range of 10-200 μm for later use;

[0048] After the homogenization treatment, the material body and water 1:1 are put into the centrifuge at 5000r / min, the centrifugation time is 50min, the filtrate is discarded, and the precipitation is set aside;

[0049] Mix the sediment and water 1:1 in a biological enzymatic hydrolysis tank, add 0.2% enzyme of the sediment mass, the reaction conditions are 30°C, pH 6.5, and react for 5 hours;

[0050] Raise the reaction temperature of the enzymolysis tank to 80°C, and keep the water for 30 minutes to inactivate the enzyme;

[0051] The feed liquid after deactivation is passed through the ultrafiltration membrane bag with a molecular we...

Embodiment 2

[0055] Soak the tuna skin and water 1:2 for 20 minutes and set aside;

[0056] Put the soaked and cleaned tuna skin into a pulverizer and process it into a slurry, and set it aside;

[0057] Enter the slurry into a colloid mill for homogenization treatment to obtain a material body with a particle size range of 10-300 μm for later use;

[0058] After the homogenization treatment, the material body and water 1:8 are put into the centrifuge at 15000r / min, the centrifugation time is 30min, the filtrate is discarded, and the sedimentation is used for later use;

[0059] Mix the precipitate and water 1:6 in the biological enzymatic hydrolysis tank evenly, add enzyme with 3.5% of the mass of the precipitate, the reaction conditions are 60°C, pH 9, and react for 1.5 hours;

[0060] Raise the reaction temperature of the enzymolysis tank to 95°C, and keep the water for 10 minutes to inactivate the enzyme;

[0061] The feed liquid after deactivation is passed through the ultrafiltrati...

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Abstract

The invention provides a preparation method and application of tuna skin high-moisturizing bioactive peptide, and belongs to the technical field of moisturizing cosmetics. The preparation method of the tuna skin high-moisturizing bioactive peptide comprises the steps that tuna skin is preliminarily crushed, and tuna skin pulp is obtained; the tuna skin pulp is subjected to micro crushing, and fine tuna skin pulp is obtained; after the fine tuna skin pulp and water are mixed, solid-liquid separation is carried out, water-soluble matter of non-collagenous protein is removed, and tuna skin precipitate is obtained; the tuna skin precipitate and enzymes are subjected to enzymatic hydrolysis in a water system, and tuna skin enzymatic hydrolysate is obtained; the enzymatic hydrolysate is separated through an ultrafiltration membrane, active polypeptide with the molecular weight smaller than 2 KD is obtained through separation, and the active peptide with the molecular weight smaller than 2 KD is the tuna skin high-moisturizing bioactive peptide. The preparation method of the tuna skin high-moisturizing bioactive peptide is simple, the process is easy to control, the tuna skin high-moisturizing bioactive peptide is suitable for industrial and large-scale production, and environmental friendliness and high safety are achieved. The prepared tuna skin high-moisturizing bioactive peptide is well applied in the field of the moisturizing cosmetics.

Description

technical field [0001] The invention relates to the technical field of moisturizing cosmetics, in particular to a preparation method and application of a highly moisturizing active peptide from tuna skin. Background technique [0002] Tuna is an important economic fish in pelagic fishery. As a deep-sea fish, tuna has delicious meat, comprehensive nutrition, high protein content and excellent coordination. During the tuna processing process, there will be a large amount of leftovers including red meat, fish head, viscera, gills and fish skin, etc., accounting for about 50% to 70% of the total mass, especially the amount of tuna skin is very large, but these leftovers are often It is directly discarded or reused as feed. At present, there is very little deep processing of tuna leftovers to extract high value-added products, resulting in a waste of resources. [0003] Tuna skin is rich in a large number of high-quality active structural proteins. It is well known in the art th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34A61K8/64A61Q19/00
CPCC12P21/06A61K8/64A61Q19/00C07K1/34
Inventor 刘卫许琦杨达峰
Owner WUHAN BEST CARRIER NANO TECH
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