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Medical biological yarn, medical biological repair mesh and preparation method thereof

A kind of biological thread and biological technology, applied in medical science, tissue regeneration, prosthesis, etc., can solve the problems of tissue regeneration mismatch, cytotoxicity, and insufficient mechanical strength, so as to reduce the incidence of inflammation and infection and achieve good mechanical properties , the effect of large mechanical strength

Pending Publication Date: 2019-02-15
上海白衣缘生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Due to the lack of mechanical strength of the acellular material patch in practical application, chemical cross-linking agents such as epoxy or glutaraldehyde need to be introduced in the process of processing, or used together with synthetic materials, which has the following disadvantages: Potential cell Toxicity, slow degradation rate, mismatch with tissue regeneration, can lead to fibrosis, chronic inflammation and other reactions

Method used

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  • Medical biological yarn, medical biological repair mesh and preparation method thereof
  • Medical biological yarn, medical biological repair mesh and preparation method thereof
  • Medical biological yarn, medical biological repair mesh and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] see Image 6 , Preparation of medical biological thread and medical biological repair mesh of porcine small intestinal submucosa soft tissue

[0057] (1) Pretreatment

[0058] Freshly slaughtered porcine small intestine tissues were cleaned, soaked in 0.5% acetic acid solution for 30 minutes, the ratio of porcine small intestine to acetic acid solution was 1:5, and the mucosal layer, muscular layer and serosa layer of porcine small intestine and jejunum were removed by physical scraping , lymph nodes, the submucosa was separated, cut into thin strips evenly in the longitudinal direction, and washed 3 times with purified water to obtain biological materials, namely small intestinal submucosa, hereinafter referred to as SIS materials.

[0059] (2) Virus inactivation

[0060] Use a mixed aqueous solution containing 1.0% peracetic acid and 15% ethanol, the ratio of the SIS material to the mixed aqueous solution is 1:10, and soak at room temperature for 100 minutes under u...

Embodiment 2

[0071] Preparation of bio-repair mesh for porcine small intestinal submucosa

[0072] (1) Pretreatment

[0073] Freshly slaughtered pig small intestine tissues were cleaned and soaked in 0.01% acetic acid solution for 120 minutes. The ratio of pig small intestine to acetic acid solution was 1:10. The mucosal layer, muscular layer and serosa layer of pig small intestine jejunum were removed by physical scraping , lymph nodes, the submucosa was separated, cut into fragments, and washed 3 times with purified water to obtain a biological material, that is, the submucosa of the small intestine, hereinafter referred to as SIS material.

[0074] (2) Virus inactivation

[0075] Use a mixed aqueous solution containing 0.5% peracetic acid and 25% ethanol, the ratio of the SIS material to the mixed aqueous solution is 1:15, and soak at room temperature for 120 minutes under ultrasonic conditions to inactivate the virus. Afterwards, it was ultrasonically washed 3 times with purified wat...

Embodiment 3

[0086] For the safety of the samples, the samples prepared in Example 1-2 were tested for immunogenic substances.

[0087] (1) Detection method of residual cells: fixed with 10% neutral formalin, embedded in paraffin, cut into thin slices of 0.4 micron, dewaxed with xylene, dehydrated with serial alcohol, stained with hematoxylin-eosin, and microscope Next, observe the cell residue and matrix fiber structure.

[0088] (2) Detection method of DNA content: according to YY / T 0606.25-2014 "Method for determination of DNA residues in biological materials of animal origin: fluorescent staining method".

[0089] (3) Detection method of α-Gal antigen content: After the sample was fixed with paraformaldehyde, it was routinely embedded in paraffin and sectioned, with a thickness of 3 μm. Immunohistochemical reaction was carried out by using the specific affinity between biotin-labeled BSI-B4 and α-Gal antigen. Judgment of staining results: dark brown-yellow particles are strongly posi...

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Abstract

The invention discloses a medical biological yarn, a medical biological repair mesh and a preparation method thereof. The medical biological yarn can be woven into the repair mesh by taking tissues ofan animal as a raw material, removing immune components such as cells and DNA and slicing the raw material into strips and twisting the strips to the yarn. A special cell-removal material is twistedto yarns to obtain greater strength, so that the yarns are hard to break. The yarns are interwoven after being woven, a stretching and tearing external force is not applied to one point but to multiple interwoven points, so that the yarn has good mechanical property. The defect that the mechanical strength of the biological repair mesh is poor and a crosslinking agent or a synthetic material is needed is overcome. Moreover, the structure of the mesh is prepared from a weaving process. The size of the pore diameter of the mesh can be adjusted easily by means of weaving parameters to meet the demand of actual applications. In addition, the medical biological yarn keeps original components such as three-dimensional structures, collagenous fibers, non-collagenous fibers and growth factors in an extracellular matrix and promotes functional reconstruction and postoperation reunion of tissues.

Description

technical field [0001] The invention relates to the field of biological materials, in particular to a medical biological thread, a medical biological repair mesh and a preparation method thereof. Background technique [0002] At present, many surgical operations use mesh. The repair and reconstruction of soft tissue represented by abdominal wall defect, chest wall defect, meningeal defect, tendon defect and burn wound is an important topic in surgery and regenerative medicine. The purpose of using the patch is to reinforce and bridge the damaged or weak soft tissue. The patch material is now relatively mature, and it is mainly divided into two categories: synthetic material patch and biological material patch. [0003] Both patches have been used clinically for many years. However, after a long period of clinical use, some disadvantages of the two types of patches have gradually emerged. First, the synthetic material patch cannot be degraded by the tissue due to its poor fl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50A61L27/58
CPCA61L27/3633A61L27/3687A61L27/50A61L27/58A61L2430/40
Inventor 韩韦红葛翠兰钱锵周秀清
Owner 上海白衣缘生物工程有限公司
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