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Method for extracting collagen using ultrasonic waves, and apparatus therefor

A collagen and ultrasonic technology, which is applied in the preparation method of peptides, the method of stress-stimulated microbial growth, chemical instruments and methods, etc., can solve the problems of waste water treatment cost and environmental pollution.

Active Publication Date: 2014-12-17
KOREA FOOD RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] On the other hand, in the past, chemical substances such as acids, alkalis, and salts were used to extract collagen, but there were problems such as environmental pollution and waste water treatment costs.

Method used

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  • Method for extracting collagen using ultrasonic waves, and apparatus therefor
  • Method for extracting collagen using ultrasonic waves, and apparatus therefor
  • Method for extracting collagen using ultrasonic waves, and apparatus therefor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1. Pretreatment of sample

[0059] As a sample, the frozen skin of sea bass supplied from O Sung Fish Farm was used. After removing the perch muscle and scales remaining on a part of the inside, the fish skin is washed with ice water to remove impurities, and then finely cut to a size of 1.0cm×1.0cm. In order to completely remove the salt-soluble protein adhering to the fish skin, after adding 0.5M NaCl solution 20 times the weight of the fish skin, stir evenly and perform centrifugation at 6,000rpm for 10 minutes to remove the supernatant liquid. The above operations were repeated three times, and all operations were performed below 4°C. After the precipitate obtained by centrifugation was washed again with purified water stored at 4°C or less, cold ethanol about 20 times the weight of the precipitate was added to the precipitate, and it was degreased while stirring at 4°C for 24 hours to make it a purified sample. Refined samples are stored below -20°...

Embodiment 2

[0060] Example 2. Extraction of Collagen

[0061] 2-1. Extraction of collagen by ultrasonic treatment

[0062] After adding approximately 200 times the weight of 0.01 to 0.5 M acetic acid to the fish skin prepared in Example 1, ultrasonic treatment was performed at 4° C. under the conditions in Table 1 below. Pulse on / off is performed at 20sec / 20sec. After centrifuging the viscous solution obtained after ultrasonic treatment at 6,000 rpm for 10 minutes, the supernatant was separated, and NaCl was added to the supernatant so as to be 5 wt % to obtain a white precipitate. The white precipitate was centrifuged and dialyzed into purified water, and then freeze-dried to extract collagen.

[0063] Table 1

[0064]

[0065] 2-2. Extraction of acid-soluble collagen

[0066] Under the conditions of the following Table 2, acetic acid of 0 to 0.5 M was added 200 times with respect to the sample weight, and it extracted while stirring at 4 degreeC for 24 hours. After the vis...

Embodiment 3

[0071] Example 3. Determination of Collagen Output Rate

[0072] Using the Biuret (biuret) method (Gornall, A, G. et al., 1949), the protein content was measured on the supernatant obtained in the above-mentioned Production Examples and Comparative Examples at 15,000 x g. The sample was obtained by centrifugation for 20 minutes. Collagen production rate was calculated according to the following formula as the ratio of protein content after ultrasonic treatment of total protein content.

[0073] Collagen production rate (%) = (protein concentration in supernatant / total protein concentration) × 100

[0074] The collagen production rate according to the treatment time is shown in Figure 1 to Figure 5 . Figures 1 to 4 Shows the production rate of collagen according to the concentration of acetic acid in Production Examples 1 to 4, Figure 5 The production rate of collagen according to the acid concentration of Comparative Examples 1 to 5 is shown. according to Figures 1...

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Abstract

The present invention relates to a method for extracting collagen using ultrasonic wave generator. More particularly, the present invention relates to a method for extracting collagen, comprising: a first step of treating fish skin with ultrasonic waves in the presence of an acid solution to obtain collagen extracts; a second step of separating collagen and the ultrasonically treated fish skin from the collagen extracts; and a step of repeating the first and second steps for the ultrasonically treated fish skin to further extract collagen. In addition, a collagen separation apparatus using ultrasonic waves according to the present invention comprises: a sample tank in which fish skin is accommodated; a separation unit which receives fish skin from the sample tank, separates collagen from the fish skin, and supplies the separated collagen back to the sample tank; and a unit for generating ultrasonic waves, which is connected to the separation unit to generate ultrasonic waves in order to separate collagen from the fish skin. The method for extracting collagen according to the present invention may enable collagen to be extracted using an acid solution having a lower concentration than those used in existing methods, may enable collagen to be extracted at a high yield rate, and may enable collagen having a high molecular weight to be extracted while maintaining the basic structure of the collagen, and not in the form of collagen hydrolysate.

Description

technical field [0001] The invention relates to a method and device for extracting collagen by ultrasonic waves. Background technique [0002] Collagen is a major protein that is contained in almost all tissues such as skin, blood vessels, bones, and internal organs, and accounts for about 30% of the proteins that make up the body. About 40% of the collagen that constitutes the human body exists in the skin, 20% is contained in the bones and cartilage, and other than that, it is widely distributed in blood vessels and internal organs. This collagen has been eaten in the form of gelatin since ancient times. This gelatin is recently decomposed by enzymes in the digestive tract and absorbed in the form of polypeptides and amino acids. These polypeptides and amino acids improve immune function, promote cell regeneration and make joints strong. , and maintain the metabolism activation and moisturizing power of the skin, thereby exerting an excellent effect on skin beauty. Colla...

Claims

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Application Information

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IPC IPC(8): C12M1/42A23L1/0562C07K1/14C07K14/78A23L29/281
CPCA23J1/002A23J1/10A23L29/281A23V2002/00
Inventor 李南赫金荣镐金贤敬禹癠焕
Owner KOREA FOOD RES INST
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