Real-time fluorescent PCR method for identifying authenticity of garter snake traditional Chinese medicinal material by adopting specific primers

Abstract

The invention belongs to the technical field of identification of traditional Chinese medicines and traditional Chinese medicinal materials, and provides a real-time fluorescent PCR method for identifying the authenticity of a garter snake traditional Chinese medicinal material by adopting specific primers, aiming at solving the problems that the existing garter snake detection method is easy to have a false positive result and cannot be applied to market rapid detection and the like. A specific primer sequence used for identifying the authenticity of the garter snake traditional Chinese medicinal material is shown as SEQ ID NO: 1 and SEQ ID NO: 2; a probe sequence is shown as SEQ ID NO: 3. The identification of the authenticity of samples can be finished through rapid DNA extraction and real-time fluorescent PCR specific amplification. The real-time fluorescent PCR method for identifying the authenticity of the garter snake traditional Chinese medicinal material by adopting the specific primers has the advantages that the primer probe double specificity has higher specificity; the fluorescence detection sensitivity is higher; the detection time of the method is short without an electrophoresis process; a pharmacopoeia detection method needs about 4 hours, and the real-time fluorescent PCR method for identifying the authenticity of the garter snake traditional Chinese medicinalmaterial by adopting the specific primers only needs 1.5 hours; the required instrument and equipment are less, and the method can be applied to a rapid detection vehicle service market, and the like.

Description

technical field [0001] The invention belongs to the technical field of identification of traditional Chinese medicines and medicinal materials, and in particular relates to a real-time fluorescent PCR method for identifying the authenticity of traditional Chinese medicinal materials of black snake by using specific primers. Background technique [0002] Black snake is a traditional precious medicinal material in my country. "Chinese Pharmacopoeia" (2015 edition) stipulates that its genuine product is the snake snake ( Zaocys dhumnades ) dry body. Most of them are caught in summer and autumn, the abdomen is cut open or the skin is peeled to keep the head and tail, the viscera are removed, the disk is made into a disk, and dried. It has the effects of expelling wind, dredging collaterals, and relieving spasm. For rheumatic obstinate numbness, numbness and spasm, apoplexy, hemiplegia, tetanus, etc. Because of its high cost of cultivation, high price, and huge demand, there a...

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Problems solved by technology

[0006] The present invention provides a real-time fluorescent PCR method using specific primers to identify the authenticity of Chinese herbal medicines of black snake in order to solve the problems that the current black snake detection m

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