Microfluidic chip, detection system and device used for rapid DNA detection

Abstract

The present invention discloses a microfluidic chip for rapid DNA detection, and the chip includes a liquid storage unit, a nucleic acid extraction unit, an amplification and detection unit and a fluid control unit. The present invention further discloses a system and a device comprising the microfluidic chip for rapid DNA detection. The microfluidic chip can achieve repeated and automated nucleic acid extraction and fast and efficient PCR amplification and real-time fluorescence detection. At the same time, by use of a disc array structure, a plurality of samples can be analyzed in parallel, so that the method is expected to be used in scientific research and clinical body fluid sample DNA rapid detection.

Description

technical field [0001] The invention relates to biomedical instruments, in particular to a microfluidic chip for rapid DNA detection, and a DNA rapid detection system and device comprising the chip. Background technique [0002] The analysis of DNA in body fluids (such as blood, urine, etc.), especially the detection of gene markers and gene variation detection is an important part of biomedical research, and it is also an important way for genetic diagnosis and genetics research. At present, the methods used for body fluid DNA analysis mainly involve sample DNA extraction, target gene PCR amplification, electrophoretic separation and detection, or real-time fluorescent PCR detection. Generally, conventional instruments and equipment are used to complete different operations. In recent years, There are also nucleic acid analysis methods and instruments using microfluidic technology, mainly the miniaturization and scale integration of routine operations (such as chip nucleic ...

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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a microfluidic chip for rapid DNA detection, and a DNA rapid detection system and device including the chip, so as to solve the problem of large consumption of DNA analysis samples and long operation period in the prior art. , cumbersome steps, low precision, etc., and solve the existing microfluidic chip and DNA detection system with insufficient automation and low analysis throughput, which is not suitable for the needs of biological laboratories, genetic research centers and clinical diagnostic laboratories. question

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